MafA Antibody

Insulin gene expression is regulated by several islet-enriched transcription factors. However, MafA is the only beta cell-specific activator. MafA selectively induces endogenous insulin transcription in non-beta cells. MafA was also first detected in the insulin-producing cells formed during the second and predominant phase of beta cell differentiation, and absent in the few insulin-positive cells found in Nkx6.1(-/-) pancreata, which lack the majority of second-phase beta cells. These results demonstrate that MafA is a potent insulin activator that is likely to function downstream of Nkx6.1 during islet insulin-producing cell development.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: Block/Neutralize, Chemotaxis, ICC/IF, IHC-Fr, IHC-P, Immunoprecipitation, WB, Western Blot.

Showing Publications 1 - 10 of 43. Show All 43 Publications. Collapse Publications.

Publications using NB400-137	Applications	Species
Becker A, Wardas B, Salah H et al. Cav?3 Regulates Ca(2+) Signaling and Insulin Expression in Pancreatic ?-Cells in a Cell-Autonomous Manner Diabetes 2021-11-01 [PMID: 34426509] (Immunoprecipitation, Western Blot, Block/Neutralize)	Immunoprecipitation, Western Blot, Block/Neutralize
E Yoshihara, C O'Connor, E Gasser, Z Wei, TG Oh, TW Tseng, D Wang, F Cayabyab, Y Dai, RT Yu, C Liddle, AR Atkins, M Downes, RM Evans Immune-evasive human islet-like organoids ameliorate diabetes Nature, 2020-08-19;0(0):. 2020-08-19 [PMID: 32814902]
Mahaddalkar PU, Scheibner K, Pfluger S et al. Generation of pancreatic ? cells from CD177(+) anterior definitive endoderm Nature Biotechnology 2020-09-01 [PMID: 32341565]
Wei Z, Yoshihara E, He N et al. Vitamin D Switches BAF Complexes to Protect B Cells Cell 2018-05-17 [PMID: 29754817] (Mouse)		Mouse
Sweeney LB, Bikoff JB, Gabitto MI et al. Origin and Segmental Diversity of Spinal Inhibitory Interneurons. Neuron 2018-01-17 [PMID: 29307712] (ICC/IF, Mouse)	ICC/IF	Mouse
Akai R, Hosoda A, Yoshino M, Iwawaki T. Constitutive role of GADD34 and CReP in cancellation of phospho-eIF2a-dependent translational attenuation and insulin biosynthesis in pancreatic b cells. Genes Cells 2015-08-28 [PMID: 26314560] (Chemotaxis, Mouse)	Chemotaxis	Mouse
Blanchet E, Van de Velde S, Matsumura S et al. Feedback Inhibition of CREB Signaling Promotes Beta Cell Dysfunction in Insulin Resistance Cell Rep. 2015-02-24 [PMID: 25704817] (WB, ICC/IF, Mouse)	WB, ICC/IF	Mouse
Alejandro EU, Gregg B, Wallen T et al. Maternal diet-induced microRNAs and mTOR underlie b cell dysfunction in offspring. J Clin. Invest. 2014-09-02 [PMID: 25180600]
Francius C, Harris A, Rucchin V et al. Identification of Multiple Subsets of Ventral Interneurons and Differential Distribution along the Rostrocaudal Axis of the Developing Spinal Cord. PLoS One. 2013-08-15 [PMID: 23967072] (IHC-Fr, ICC/IF, Mouse)	IHC-Fr, ICC/IF	Mouse
Campbell-Thompson ML, Heiple T, Montgomery E et al. Staining protocols for human pancreatic islets. J Vis Exp 2012-05-01 [PMID: 22665223] (IHC-P, Human)	IHC-P	Human
Hu J, Huang T, Li T et al. c-Maf is required for the development of dorsal horn laminae III/IV neurons and mechanoreceptive DRG axon projections. J Neurosci 2012-04-01 [PMID: 22514301]
Lausier J, Diaz WC, Roskens V et al. Vagal control of pancreatic beta-cell proliferation. Am J Physiol Endocrinol Metab 2010-11-01 [PMID: 20716695]
Artner I, Hang Y, Mazur M et al. MafA and MafB regulate genes critical to beta-cells in a unique temporal manner. Diabetes. 2010-07-13 [PMID: 20627934]
Kang HS, Kim YS, ZeRuth G et al. Transcription factor Glis3, a novel critical player in the regulation of pancreatic beta-cell development and insulin gene expression. Mol Cell Biol 2009-12-01 [PMID: 19805515]
Takeuchi et al. Genes to Cells 14 (8):941-947. 2009-01-01 [PMID: 19624757]
Yang et al. Nucleic Acids Res. 37 (8):2529-2538. 2009-01-01 [PMID: 19264802]
Guo et al. J.Biol.Chem 284 (2):759-765. 2009-01-01 [PMID: 19004825]
Zhou et al. Nature 455 (7213):627-632. 2008-01-01 [PMID: 18754011]
Vanhoose et al. J.Biol.Chem 283 (33):22612-22619. 2008-01-01 [PMID: 18522939]
Artner et al. J Endocrinol 198 (2):271-279. 2008-01-01 [PMID: 18515495]
Goldsworthy et al. Diabetes 57 (8):2234-2244. 2008-01-01 [PMID: 18477811]
Wang et al. Diabetes 57 (8):2137-2148. 2008-01-01 [PMID: 18469203]
Seo et al. Endocrinology 149 (8):3832-3841. 2008-01-01 [PMID: 18450959]
Wang, Y, Torres-Gonzalez, M, Tripathy, S et al. Elevated hepatic fatty acid elongase-5 activity affects multiple pathways controlling hepatic lipid and carbohydrate composition. J Lipid Res;49(7):1538-52. 2008-07-01 [PMID: 18376007]
Matsuoka TA, Kaneto H, Stein R et al. MafA regulates expression of genes important to islet beta-cell function. Mol Endocrinol 2007-11-01 [PMID: 17636040]
Doyle Sussel. Diabetes 56 (8):1999-2007. 2007-01-01 [PMID: 17456846]
Artner et al. PNAS 104 (10):3853-3858. 2007-01-01 [PMID: 17360442]
Park et al. Diabetes 56 (2):431-437. 2007-01-01 [PMID: 17259388]
Doyle et al. Development 134 (3):515-523. 2007-01-01 [PMID: 17202186]
Sugiyama T, Rodriguez RT, McLean GW et al. Conserved markers of fetal pancreatic epithelium permit prospective isolation of islet progenitor cells by FACS. Proc Natl Acad Sci U S A 2007-01-01 [PMID: 17190805]
Tweedie et al. Diabetes 55 (12):3264-3270. 2006-01-01 [PMID: 17130469]
Nakamura et al. BBRC 350 (1):68-73. 2006-01-01 [PMID: 16996478]
Shimohata et al. Biochem Biophys Res Comm 346 (3):671-680. 2006-01-01 [PMID: 16780794]
Wichroski MJ, Robb GB, Rana TM et al. Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies. PLoS Pathog 2006-05-01 [PMID: 16699599]
Ueki et al. Nat Genet 38 (5):583-588. 2006-01-01 [PMID: 16642022]
Ye et al. Diabetes 55 (3):742-750. 2006-01-01 [PMID: 16505238]
Smart et al. PLoS Biology 4 (2). 2006-01-01 [PMID: 16435884]
Hagman DK, Hays LB, Parazzoli SD et al. Palmitate inhibits insulin gene expression by altering PDX-1 nuclear localization and reducing MafA expression in isolated rat islets of Langerhans. J Biol Chem 2005-09-01 [PMID: 15944145]
Zhao L, Guo M, Matsuoka TA et al. The islet beta cell-enriched MafA activator is a key regulator of insulin gene transcription. J Biol Chem 2005-03-01 [PMID: 15665000]
Harmon JS, Stein R, Robertson RP et al. Oxidative stress-mediated, post-translational loss of MafA protein as a contributing mechanism to loss of insulin gene expression in glucotoxic beta cells. J Biol Chem 2005-03-01 [PMID: 15664999]
Pino MF, Ye DZ, Linning KD et al. Elevated glucose attenuates human insulin gene promoter activity in INS-1 pancreatic beta-cells via reduced nuclear factor binding to the A5/core and Z element. Mol Endocrinol 2005-05-01 [PMID: 15650027]
Matsuoka TA, Artner I, Henderson E et al. The MafA transcription factor appears to be responsible for tissue-specific expression of insulin. Proc Natl Acad Sci U S A 2004-03-01 [PMID: 14973194]
Matsuoka TA, Zhao L, Artner I et al. Members of the large Maf transcription family regulate insulin gene transcription in islet beta cells. Mol Cell Biol 2003-09-01 [PMID: 12917329]
Show All 43 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Yves Heremans	IHC-P	Mouse	09/21/2015	View Summary Application Immunohistochemistry-Paraffin Sample Tested Mouse Pancreas Species Mouse

Array NB400-137

• MafA Antibodies
• MafA Lysate
• MafA Protein