Home » Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 » Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibodies » Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [CoraFluor™ 1]

Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-06855AF350
Alexa Fluor 405
NBP3-06855AF405
Alexa Fluor 488
NBP3-06855AF488
Alexa Fluor 532
NBP3-06855AF532
Alexa Fluor 594
NBP3-06855AF594
Alexa Fluor 647
NBP3-06855AF647
Alexa Fluor 700
NBP3-06855AF700
Alexa Fluor 750
NBP3-06855AF750
Biotin
NBP3-06855B
DyLight 350
NBP3-06855UV
DyLight 405
NBP3-06855V
DyLight 488
NBP3-06855G
DyLight 550
NBP3-06855R
DyLight 594
NBP3-06855DL594
DyLight 650
NBP3-06855C
DyLight 680
NBP3-06855FR
DyLight 755
NBP3-06855IR
FITC
NBP3-06855F
Janelia Fluor 525
NBP3-06855JF525
Janelia Fluor 549
NBP3-06855JF549
Janelia Fluor 646
NBP3-06855JF646
Janelia Fluor 669
NBP3-06855JF669
Unconjugated
NBP3-06855
mFluor Violet 450 SE
NBP3-06855MFV450
mFluor Violet 500 SE
NBP3-06855MFV500
mFluor Violet 610 SE
NBP3-06855MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-06855
BSA Free
NBP3-06855SS
BSA Free
NBP3-06855
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Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Partial recombinant protein made to an internal portion of the human JMJD3 protein (amino acids 1520-1682) [UniProt O15054]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM6B
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [CoraFluor™ 1]

  • EC 1.14.11
  • EC 1.14.11.-
  • JmjC domain-containing protein 3
  • JMJD3
  • JMJD3lysine-specific demethylase 6B
  • jumonji domain containing 3, histone lysine demethylase
  • Jumonji domain-containing protein 3
  • KDM6B
  • KIAA0346jumonji domain containing 3
  • Lysine (K)specific Demethylase 6B
  • Lysine (K)-specific Demethylase 6B
  • Lysine demethylase 6B

Background

JMJD3 is a histone demethylase that specifically demethylates trimethylated and dimethylated 'Lys-27' of histone H3, thereby playing a central role in histone code. JMJD3 is also important for the regulation of posterior development by regulating HOX gene expression. JMJD3 is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. It has also been shown to be essential to mammalian epidermal differentiation and is upregulated in prostate cancer.

JMJD3 antibodies are useful tools for epigenetics research and specifically for histone modification studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody (NBP3-06855CL1) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KDM6B