Home » LSS » LSS Lysates » LSS Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

LSS Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: LSS Overexpression Lysate (Adult Normal) [NBP2-08981] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LSS.
Western Blot: LSS Overexpression Lysate (Adult Normal) [NBP2-08981] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LSS.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

LSS Overexpression Lysate Summary

Description

LSS Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC214463

Accession#: NM_001001438

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
LSS

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
83.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for LSS Overexpression Lysate

  • EC 5.4.99.7
  • FLJ350152,3-epoxysqualene--lanosterol cyclase
  • FLJ39450
  • FLJ46393
  • hOSC
  • human lanosterol synthase, EC 5.4.99.710EC 5.4.99
  • lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase)2,3-epoxysqualene-lanosterol cyclase
  • lanosterol synthase
  • LSS
  • OSC
  • OSCFLJ25486
  • Oxidosqualene--lanosterol cyclase

Background

LSS is encoded by this gene catalyzes the conversion of (S)-2,3 oxidosqualene to lanosterol. The encoded protein is a member of the terpene cyclase/mutase family and catalyzes the first step in the biosynthesis of cholesterol, steroid hormones, and vitamin D. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Cathepsin C/DPPI
Antibody [Unconjugate ...
AF1034
Western Blot Cathepsin C/DPPI Antibody [Unconjugated]
Immunohistochemistry Cathepsin C/DPPI Antibody [Unconjugated]
Western Blot Cathepsin C/DPPI Antibody [Unconjugated]
(14 Publications)
Species: Mu, Rt
Applications: IHC, Simple Western, WB
FDFT1 Antibody
(OTI2F10)
NBP2-01170
Western Blot FDFT1 Antibody (OTI2F10)
Immunohistochemistry-Paraffin FDFT1 Antibody (OTI2F10)
Immunohistochemistry-Paraffin FDFT1 Antibody (OTI2F10)
Species: Hu, Pm, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, WB
SQLE Antibody -
Azide and BSA Free
NBP2-93808
Immunocytochemistry/ Immunofluorescence SQLE Antibody - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence SQLE Antibody - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence SQLE Antibody - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(818 Publications)
Species: Hu
Applications: BA
Sulfamidase/SGSH
Antibody
NBP1-83164
Western Blot Sulfamidase/SGSH Antibody
Immunohistochemistry-Paraffin Sulfamidase/SGSH Antibody
Immunohistochemistry-Paraffin Sulfamidase/SGSH Antibody
Species: Hu
Applications: IHC, IHC-P, WB
SPAG9 Antibody
NBP1-85393
Immunocytochemistry/ Immunofluorescence SPAG9 Antibody
Immunohistochemistry-Paraffin SPAG9 Antibody
Immunohistochemistry-Paraffin SPAG9 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
PANK2 Antibody
(OTI3H9)
NBP2-02677
Flow Cytometry PANK2 Antibody (OTI3H9)
Flow Cytometry PANK2 Antibody (OTI3H9)
Western Blot PANK2 Antibody (OTI3H9)
Species: Ca, Hu, Pm, Mu, Pm, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
GFER/ALR Antibody
(829812)
MAB7940
Immunohistochemistry GFER/ALR Antibody (829812)
Western Blot GFER/ALR Antibody (829812)
(1 Publication)
Species: Hu
Applications: IHC, WB
NOC2L Antibody
NBP1-92190
Immunocytochemistry/ Immunofluorescence NOC2L Antibody
Western Blot NOC2L Antibody
Immunohistochemistry-Paraffin NOC2L Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, Simple Western, WB
MID1 Antibody
NBP1-26612
Western Blot MID1 Antibody
(2 Publications)
Species: Hu
Applications: IP (-), WB
Osteocalcin
Antibody (190125)
MAB1419
Intracellular Staining by Flow Cytometry Osteocalcin Antibody (190125)
Intracellular Staining by Flow Cytometry Osteocalcin Antibody (190125)
Immunocytochemistry Osteocalcin Antibody (190125)
(3 Reviews)
(56 Publications)
Species: Hu, Rt
Applications: CyTOF-ready, ICC, IHC, ICFlow
Glutaminyl-peptide
Cyclotransferase/QPCT ...
NBP1-81838
Western Blot Glutaminyl-peptide Cyclotransferase/QPCT Antibody
Immunohistochemistry Glutaminyl-peptide Cyclotransferase/QPCT Antibody
Immunohistochemistry-Paraffin Glutaminyl-peptide Cyclotransferase/QPCT Antibody
(1 Publication)
Species: Hu, Mu
Applications: IHC, IHC-P, WB
hnRNP C1 + C2
Antibody (CL2593)
NBP2-36776
Western Blot hnRNP C1 + C2 Antibody (CL2593)
Immunohistochemistry hnRNP C1 + C2 Antibody (CL2593)
Immunohistochemistry hnRNP C1 + C2 Antibody (CL2593)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
LIGHT/TNFSF14
[Unconjugated]
664-LI
SDS-PAGE LIGHT/TNFSF14 [Unconjugated]
Bioactivity LIGHT/TNFSF14 [Unconjugated]
(21 Publications)
Species: Hu
Applications: BA

Acetylcholinesterase/A
NB100-1519
Flow Cytometry Acetylcholinesterase/ACHE Antibody
Western Blot Acetylcholinesterase/ACHE Antibody
Immunocytochemistry/ Immunofluorescence Acetylcholinesterase/ACHE Antibody
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC-FrFl, PEP-ELISA, WB
Dehydrodolichyl
Diphosphate Synthase ...
NBP1-87964
Immunohistochemistry-Paraffin Dehydrodolichyl Diphosphate Synthase Antibody
Immunohistochemistry-Paraffin Dehydrodolichyl Diphosphate Synthase Antibody
Immunohistochemistry-Paraffin Dehydrodolichyl Diphosphate Synthase Antibody
(1 Publication)
Species: Hu
Applications: IHC, IHC-P
Osteopontin/OPN
Antibody [Unconjugate
AF808
Immunocytochemistry Osteopontin/OPN Antibody [Unconjugated]
Neutralization Osteopontin/OPN Antibody [Unconjugated]
Immunohistochemistry Osteopontin/OPN Antibody [Unconjugated]
(2 Reviews)
(207 Publications)
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, IHC, Neut, WB
eNOS Antibody -
BSA Free
NB300-500
Western Blot eNOS Antibody - BSA Free
Immunohistochemistry-Paraffin eNOS Antibody - BSA Free
Immunohistochemistry-Paraffin eNOS Antibody - BSA Free
(3 Reviews)
(28 Publications)
Species: Am, Bv, Ca, Dr, Hu, Mu, Po, Rt, Sh
Applications: IHC, IHC-Fr, IHC-P, WB

Publications for LSS Lysate (NBP2-08981) (0)

There are no publications for LSS Lysate (NBP2-08981).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for LSS Lysate (NBP2-08981) (0)

There are no reviews for LSS Lysate (NBP2-08981). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for LSS Lysate (NBP2-08981) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional LSS Products

Blogs on LSS

There are no specific blogs for LSS, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005
Osteopontin/OPN was detected in immersion fixed C2C12 mouse myoblast cell line (left panel, positive stain) and mouse splenocytes (right panel, negative stain) using Goat Anti-Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and secreted molecule. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Osteopontin/OPN Antibody [Unconjugated]
AF808

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our LSS Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol LSS