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Human LPAR3/LPA3/EDG-7 ELISA Kit (Colorimetric)

Name: Human LPAR3/LPA3/EDG-7 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-40052

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ELISA: Human LPAR3/LPA3/EDG-7 ELISA Kit (Colorimetric) [NBP3-40052] - Standard Curve Reference

Product Details

Summary

Reactivity	HuSpecies Glossary
Applications	ELISA
Suitable Sample Type	Tissue homogenates, cell lysates and other biological fluids
Standard Curve Range	0.312 - 20 ng/mL (example only; lot dependent)
Sensitivity	0.105 ng/mL (example only; lot dependent)

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Human LPAR3/LPA3/EDG-7 ELISA Kit (Colorimetric) Summary

Description	Assay Length: 4.5 hours
Standard Curve Range	0.312 - 20 ng/mL (example only; lot dependent)
Sensitivity	0.105 ng/mL (example only; lot dependent)
Assay Type	Sandwich ELISA
Inter-Assay	%CV < 12 (example only; lot dependent)
Intra-Assay	%CV < 10 (example only; lot dependent)
Sample Volume	100 uL
Kit Type	ELISA Kit (Colorimetric)
Gene	LPAR3

Applications/Dilutions

Dilutions

ELISA

Packaging, Storage & Formulations

Storage

Storage of components varies. See protocol for specific instructions.

Kit Components

Components

Detection Reagent A
Detection Reagent B
Diluent Buffer
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Alternate Names for Human LPAR3/LPA3/EDG-7 ELISA Kit (Colorimetric)

EDG7
Edg-7
EDG7FLJ98231
GPCR
HOFNH30
LPA-3
LPA3LPA receptor 3
LPAR3
lysophosphatidic acid G-protein-coupled receptor7,LPA receptor EDG7
lysophosphatidic acid receptor 3
Lysophosphatidic acid receptor Edg-7
RP4-678I3

Background

EDG7, a Lysophospholipid/Lysosphingolipid Receptor, was identified by RT-PCR from Jurkat T-cells. It is activated by unsaturated lysophosphatidic acid LPA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for LPAR3/LPA3/EDG-7 ELISA Kit (NBP3-40052) (0)

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Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for EphB2 at approximately 139 and 146 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

EphB2 Antibody [Unconjugated]
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Bioinformatics

Gene Symbol

LPAR3

Popular Products	Company Information	Support	Stay Connected
Antibodies Secondary Antibodies Isotype Controls Proteins and Peptides ELISA Kits Sample Size New Products	About Us Careers Novus Guarantee Contact Us Our Team	Technical Support FAQs Help Finding Antibodies Protocols

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Human LPAR3/LPA3/EDG-7 ELISA Kit (Colorimetric)

Species Glossary

Images

Product Details

Order Details

Human LPAR3/LPA3/EDG-7 ELISA Kit (Colorimetric) Summary

Applications/Dilutions

Packaging, Storage & Formulations

Kit Components

Alternate Names for Human LPAR3/LPA3/EDG-7 ELISA Kit (Colorimetric)

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for LPAR3/LPA3/EDG-7 ELISA Kit (NBP3-40052) (0)

Reviews for LPAR3/LPA3/EDG-7 ELISA Kit (NBP3-40052) (0)

Product General Protocols

FAQs for LPAR3/LPA3/EDG-7 ELISA Kit (NBP3-40052) (0)

Additional LPAR3/LPA3/EDG-7 Products

Research Areas for LPAR3/LPA3/EDG-7 ELISA Kit (NBP3-40052)

Blogs on LPAR3/LPA3/EDG-7

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Bioinformatics