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Lipocalin-2/NGAL Antibody (228418) [Unconjugated]

 (18 Publications)
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Western blot shows lysates of mouse spleen tissue, mouse uterus tissue, and mouse epididymis tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Lipocalin-2/NGAL Monoclonal Antibody (Catalog # MAB1857) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lipocalin-2/NGAL at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse spleen tissue, mouse uterus tissue, and mouse epididymis tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Lipocalin-2/NGAL Monoclonal Antibody (Catalog # MAB1857) ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clone
228418
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAM1857
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB1857-SP
Carrier Free
MAB1857-500
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Lipocalin-2/NGAL Antibody (228418) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Lipocalin-2
aa 21-200
Accession # P11672
Specificity
Detects recombinant mouse (rm)Lipocalin-2 in direct ELISAs and Western blots. In these formats, does not cross-react with rhLipocalin-1 or rhLipocalin‑2.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
LCN2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
MAB1857 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lipocalin-2/NGAL Antibody (228418) [Unconjugated]

  • 24p3
  • 25 kDa alpha-2-microglobulin-related subunit of MMP-9
  • HNL
  • LCN2
  • lipocalin 2 (oncogene 24p3)
  • lipocalin 2
  • Lipocalin2
  • Lipocalin-2
  • migration-stimulating factor inhibitor
  • MSFI
  • neutrophil gelatinase-associated lipocalin
  • NGAL
  • NGALlipocalin-2
  • Oncogene 24p3
  • p25
  • Siderocalin
  • Uterocalin

Background

Lipocalin-2, also known as Neutrophil Gelatin-Associated Lipocalin (NGAL) and Siderocalin, mediates the innate immune response to bacterial infection by sequestering iron-laden siderophores.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Lipocalin-2/NGAL
Antibody (228418) [Un ...
MAB1857
Lipocalin-2/NGAL Antibody (228418) [Unconjugated]
Species: Mu
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Publications for Lipocalin-2/NGAL Antibody (MAB1857)(18)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 6 applications: ICC, IHC, IHC-P, Immunoprecipitation, Neutralization, Western Blot.


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ICC
(1)
IHC
(3)
IHC-P
(1)
Immunoprecipitation
(2)
Neutralization
(2)
Western Blot
(4)
All Applications
Filter By Species
Mouse
(12)
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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using MAB1857 Applications Species
Cheng, M;Zheng, Y;Fan, Y;Yan, P;Zhao, W; The contribution of IL-17A-dependent low LCN2 levels to Helicobacter pylori infection: Insights from clinical and experimental studies International immunopharmacology 2023-09-16 [PMID: 37722259] (Neutralization, Mouse) Neutralization Mouse
Mengistu Lemecha, Jaya Prakash Chalise, Yuki Takamuku, Guoxiang Zhang, Takahiro Yamakawa, Garrett Larson et al. Lcn2 mediates adipocyte-muscle-tumor communication and hypothermia in pancreatic cancer cachexia Molecular Metabolism 12/1/2022 [PMID: 36243318]
C Huisman, MA Norgard, PR Levasseur, SM Krasnow, MGP van der Wi, B Olson, DL Marks Critical changes in hypothalamic gene networks in response to pancreatic cancer as found by single-cell RNA sequencing Molecular Metabolism, 2022-01-11;0(0):101441. 2022-01-11 [PMID: 35031523] (IHC, Mouse) IHC Mouse
C Mertens, L Kuchler, A Sola, R Guiteras, S Grein, B Brüne, A von Knethe, M Jung Macrophage-Derived Iron-Bound Lipocalin-2 Correlates with Renal Recovery Markers Following Sepsis-Induced Kidney Damage Int J Mol Sci, 2020-10-13;21(20):. 2020-10-13 [PMID: 33065981] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
A Braga, S Bandiera, J Verheyen, R Hamel, C Rutigliani, F Edenhofer, JA Smith, S Pluchino Combination of In Situ Lcn2 pRNA-RNAi Nanotherapeutics and iNSC Transplantation Ameliorates Experimental SCI in Mice Mol. Ther., 2020-08-05;0(0):. 2020-08-05 [PMID: 32877696] (Western Blot, Mouse) Western Blot Mouse
Shuai Shao, Hui Fang, Erle Dang, Ke Xue, Jieyu Zhang, Bing Li et al. Neutrophil Extracellular Traps Promote Inflammatory Responses in Psoriasis via Activating Epidermal TLR4/IL-36R Crosstalk Frontiers in Immunology 4/5/2019 [PMID: 31024570]
Singh V, Yeoh BS, Chassaing B et al. Dysregulated Microbial Fermentation of Soluble Fiber Induces Cholestatic Liver Cancer. Cell. 10/18/2018 [PMID: 30340040]
D Fischer, C Seifen, P Baer, M Jung, C Mertens, B Scheller, K Zacharowsk, R Hofmann, TJ Maier, A Urbschat The Fibrin Cleavage Product B?15-42 Channels Endothelial and Tubular Regeneration in the Post-acute Course During Murine Renal Ischemia Reperfusion Injury Front Pharmacol, 2018-04-27;9(0):369. 2018-04-27 [PMID: 29755348] (IHC, Mouse) IHC Mouse
JA Smith, A Braga, J Verheyen, S Basilico, S Bandiera, C Alfaro-Cer, L Peruzzotti, D Shu, F Haque, P Guo, S Pluchino RNA Nanotherapeutics for the Amelioration of Astroglial Reactivity Mol Ther Nucleic Acids, 2017-11-24;10(0):103-121. 2017-11-24 [PMID: 29499926] (ICC, Mouse) ICC Mouse
You-Jin Choi, Dong Zhou, Anne Caroline S. Barbosa, Yongdong Niu, Xiudong Guan, Meishu Xu et al. Activation of Constitutive Androstane Receptor Ameliorates Renal Ischemia-Reperfusion–Induced Kidney and Liver Injury Molecular Pharmacology 3/1/2018 [PMID: 29351922]
Show All 18 Publications.

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Bioinformatics

Gene Symbol LCN2
Uniprot