![Western Blot: LIF Antibody [NBP1-76554] - Western Blot Validation in Rat Lung Loading: 15 ug of lysates per lane. Antibodies: LIF NBP1-76554, (1: 1 ug/mL, 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/LIF-Antibody-Western-Blot-NBP1-76554-img0006.jpg "Western Blot: LIF Antibody [NBP1-76554] - Western Blot Validation in Rat Lung Loading: 15 ug of lysates per lane. Antibodies: LIF NBP1-76554, (1: 1 ug/mL, 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, B/N |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a 16 amino acid synthetic peptide near the center of human LIF. The immunogen is located within amino acids 50 - 100 of LIF. Amino Acid Squence: LYYTAQGEPFPNNLDK |
| Specificity | At least two isoforms of LIF are known to exist. |
| Predicted Species | Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | LIF |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Use in blocking/neutralizing reported in scientific literature (PMID: 26491045). |
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| Theoretical MW | 25 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
| Publication using NBP1-76554 | Applications | Species |
|---|---|---|
| Gao S Carson JA. Lewis lung carcinoma regulation of mechanical stretch-induced protein synthesis in cultured myotubes. Am. J. Physiol., Cell Physiol. 2016-01-01 [PMID: 26491045] (B/N, Mouse) | B/N | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for LIF Antibody (NBP1-76554)Find related products by research area.
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KLF4 as a transcription factor in stem cell differentiation Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel... Read full blog post. |
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![Western Blot: LIF Antibody [NBP1-76554] - 3T3 cell lysate with LIF antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/LIF-Antibody-Western-Blot-NBP1-76554-img0001.jpg "Western Blot: LIF Antibody [NBP1-76554] - 3T3 cell lysate with LIF antibody at (A) 1 and (B) 2 ug/mL.")
![Western Blot: LIF Antibody [NBP1-76554] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: LIF NBP1-76554, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/LIF-Antibody-Western-Blot-NBP1-76554-img0003.jpg "Western Blot: LIF Antibody [NBP1-76554] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: LIF NBP1-76554, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: LIF Antibody [NBP1-76554] - Western Blot Validation with Recombinant Protein. Loading: 15 ug of lysates per lane. Antibodies: LIF NBP1-76554, (1: 1 ug/mL, 2: 2ug/mL, 3: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/LIF-Antibody-Western-Blot-NBP1-76554-img0004.jpg "Western Blot: LIF Antibody [NBP1-76554] - Western Blot Validation with Recombinant Protein. Loading: 15 ug of lysates per lane. Antibodies: LIF NBP1-76554, (1: 1 ug/mL, 2: 2ug/mL, 3: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: LIF Antibody [NBP1-76554] - Western Blot Validation in Mouse Cell Lines and Tissue. Loading: 15 ug of lysates per lane. Antibodies: LIF NBP1-76554, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/LIF-Antibody-Western-Blot-NBP1-76554-img0005.jpg "Western Blot: LIF Antibody [NBP1-76554] - Western Blot Validation in Mouse Cell Lines and Tissue. Loading: 15 ug of lysates per lane. Antibodies: LIF NBP1-76554, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunocytochemistry/Immunofluorescence: LIF Antibody [NBP1-76554] - Immunofluorescent analysis of 4% paraformaldehydefixed 3T3 cells labeling LIF with NBP1-76554 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/LIF-Antibody-Immunofluorescence-NBP1-76554-img0002.jpg "Immunocytochemistry/Immunofluorescence: LIF Antibody [NBP1-76554] - Immunofluorescent analysis of 4% paraformaldehydefixed 3T3 cells labeling LIF with NBP1-76554 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).")
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![N/A gp130/CD130 [HRP]](https://images.novusbio.com/images2/DATA_gp130_DGP00_ELISA_706.jpg)
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![SDS-PAGE IL-3 [Unconjugated]](https://images.novusbio.com/images2/IL-3_203-IL_345.jpg)
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![SEC-MALS IL-3 [Unconjugated]](https://images.novusbio.com/images/203-il_recombinant-human-il-3-protein-sec-mals-133202482656..jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")