Home » LIF » LIF Antibodies » LIF Antibody (39N7D10) - Azide and BSA Free
Novus Biologicals products are now on bio-techne.com

LIF Antibody (39N7D10) - Azide and BSA Free

 Submit a Review

Images

 
(
Enlarge)
Western Blot: LIF Antibody (39N7D10) - Azide and BSA Free [NBP2-80834] - WB validation of LIF antibody (clone 39N7D10) on (A) full-length recombinant Lif protein, (B) mouse spleen lysate and (C) human spleen lysate. 3 ug/mL concentration of primary antibody, Goat anti-rat IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this assay. Image from the standard format of this antibody.
Western Blot: LIF Antibody (39N7D10) - Azide and BSA Free [NBP2-80834] - WB validation of LIF antibody (clone 39N7D10) on (A) full-length recombinant Lif protein, (B) mouse spleen lysate and (C) human spleen lysate. 3 ...read more
Immunocytochemistry: LIF Antibody (39N7D10) - Azide and BSA Free [NBP2-80834] - Tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Image from the standard format of this antibody.
Immunohistochemistry: LIF Antibody (39N7D10) - Azide and BSA Free [NBP2-80834] - Tissue section of normal human kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Expected membrane- cytoplasmic ...read more
Western Blot: LIF Antibody (39N7D10) - Azide and BSA Free [NBP2-80834] - LIF expression in human primary T-cells and cancer cell lines: Jurkat and K562. Image from verified customer review. Image from the standard ...read more
Immunohistochemistry: LIF Antibody (39N7D10) - Azide and BSA Free [NBP2-80834] - Tissue section of adenocarcinoma of human rectum using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The cancer cells as well as ...read more
Immunohistochemistry: LIF Antibody (39N7D10) - Azide and BSA Free [NBP2-80834] - Tissue section of mouse colon using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The columnar epithelial cells of the crypts ...read more
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - Azide and BSA Free [NBP2-80834] - Tissue section of mouse kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Very intense immune positivity of LIF ...read more
Immunohistochemistry: LIF Antibody (39N7D10) - Azide and BSA Free [NBP2-80834] - Tissue section of normal human prostate using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Cell surface/membrane- cytoplasmic ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
39N7D10
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-27406AF350
Alexa Fluor 405
NBP2-27406AF405
Alexa Fluor 488
NBP2-27406AF488
Alexa Fluor 532
NBP2-27406AF532
Alexa Fluor 594
NBP2-27406AF594
Alexa Fluor 647
NBP2-27406AF647
Alexa Fluor 700
NBP2-27406AF700
Alexa Fluor 750
NBP2-27406AF750
Biotin
NBP2-27406B
DyLight 350
NBP2-27406UV
DyLight 405
NBP2-27406V
DyLight 488
NBP2-27406G
DyLight 550
NBP2-27406R
DyLight 594
NBP2-27406DL594
DyLight 650
NBP2-27406C
DyLight 680
NBP2-27406FR
DyLight 755
NBP2-27406IR
FITC
NBP2-27406F
HRP
NBP2-27406H
Janelia Fluor 549
NBP2-27406JF549
Janelia Fluor 646
NBP2-27406JF646
Unconjugated
NBP2-27406
mFluor Violet 450 SE
NBP2-27406MFV450
mFluor Violet 500 SE
NBP2-27406MFV500
mFluor Violet 610 SE
NBP2-27406MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-27406
BSA Free
NBP2-27406
BSA Free
NBP2-27406SS
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

LIF Antibody (39N7D10) - Azide and BSA Free Summary

Immunogen
A recombinant murine Lif protein containing amino acids 24-203 was used as the immunogen for this antibody.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
LIF
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 3 - 5 ug/ml
Application Notes
The Lif protein can be highly glycosylated and has been observed between 22 kD and 34 kD in western blotting. Staining in IHC-P is enhanced through antigen retrieval using 10 mM Sodium Citrate buffer, pH 6.0.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for LIF Antibody (39N7D10) - Azide and BSA Free

  • CDF
  • D Factor
  • DIA
  • differentiation inhibitory activity
  • differentiation stimulating factor
  • Differentiation-stimulating factor
  • Emfilermin
  • HILDA
  • HILDAcholinergic differentiation factor
  • leukemia inhibitory factor (cholinergic differentiation factor)
  • leukemia inhibitory factor
  • LIF
  • Melanoma-derived LPL inhibitor
  • MLPLI

Background

Lif has long been recognized as a cytokine which promotes murine germ cell growth in the absence of feeder cell layers in mouse cells (1-3)Characterized initially as an inhibitor of differentiation in M1 murine monocytic leukemia lines (hence the name Leukemia Inhibitory Factor),Lif is now recognized as a key factor for maintaining stem cells in the undifferentiated state- at least in mouse embryonic stem cells (4)Acting through the Lif receptor and the induced activation of STAT3, murine ESCs can be maintained in vitro in undifferentiated stateHowever, human ESCs, although functionally stimulated with Lif, are not similarly maintained as undifferentiatedThis illustrates a different functional program for Lif in mice and humans in maintenance of stem cellsAn IL-6 family member, Lif is an important pleiotropic cytokine with activity on cells of hematopoietic and non-hematopoietic origin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Oncostatin M/OSM
[Unconjugated]
8475-OM/CF
Oncostatin M/OSM [Unconjugated]
(1 Publication)
Species: Hu
Applications: BA
gp130/CD130 [HRP]
DGP00
N/A gp130/CD130 [HRP]
N/A gp130/CD130 [HRP]
N/A gp130/CD130 [HRP]
(14 Publications)
Species: Hu
Applications: ELISA
LIFR alpha
Antibody [Unconjugate
AF-249-NA
Neutralization LIFR alpha Antibody [Unconjugated]
(16 Publications)
Species: Hu
Applications: Neut, WB
CNTF [Unconjugated]
257-NT
SDS-PAGE CNTF [Unconjugated]
Bioactivity CNTF [Unconjugated]
(58 Publications)
Species: Hu
Applications: BA
STAT3 Antibody
(232209) [Unconjugate
MAB1799
Knockout Validated STAT3 Antibody (232209) [Unconjugated]
Western Blot STAT3 Antibody (232209) [Unconjugated]
Knockout Validated STAT3 Antibody (232209) [Unconjugated]
(22 Publications)
Species: Hu, Mu, Rt
Applications: ICC, IP, ICFlow, KO, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(803 Publications)
Species: Hu
Applications: BA
LRPPRC Antibody
NBP1-83349
Western Blot LRPPRC Antibody
Immunocytochemistry/ Immunofluorescence LRPPRC Antibody
Immunohistochemistry-Paraffin LRPPRC Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
IL-11 [HRP]
D1100
N/A IL-11 [HRP]
N/A IL-11 [HRP]
N/A IL-11 [HRP]
(29 Publications)
Species: Hu
Applications: ELISA
CCM2 Antibody
(OTI1E9)
NBP2-01134
Western Blot CCM2 Antibody (OTI1E9)
Immunohistochemistry-Paraffin CCM2 Antibody (OTI1E9)
Immunohistochemistry-Paraffin CCM2 Antibody (OTI1E9)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Western Blot HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
SCF/c-kit Ligand
[Unconjugated]
255-SC
SCF/c-kit Ligand [Unconjugated]
(194 Publications)
Species: Hu
Applications: BA
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
FGF
basic/FGF2/bFGF [Unco
233-FB
FGF basic/FGF2/bFGF [Unconjugated]
(530 Publications)
Species: Hu
Applications: BA
IL-1 beta/IL-1F2
[Unconjugated]
201-LB
SDS-PAGE IL-1 beta/IL-1F2 [Unconjugated]
Bioactivity IL-1 beta/IL-1F2 [Unconjugated]
SEC-MALS IL-1 beta/IL-1F2 [Unconjugated]
(572 Publications)
Species: Hu
Applications: BA
EGF [Unconjugated]
236-EG
SDS-PAGE EGF [Unconjugated]
Bioactivity EGF [Unconjugated]
Cell Culture EGF [Unconjugated]
(780 Publications)
Species: Hu
Applications: BA
IL-3 [Unconjugated]
203-IL
SDS-PAGE IL-3 [Unconjugated]
Bioactivity IL-3 [Unconjugated]
SEC-MALS IL-3 [Unconjugated]
(204 Publications)
Species: Hu
Applications: BA
Oct-3/4 Antibody
[Unconjugated]
AF1759
Chromatin Immunoprecipitation (ChIP) Oct-3/4 Antibody [Unconjugated]
Immunocytochemistry Oct-3/4 Antibody [Unconjugated]
Simple Western Oct-3/4 Antibody [Unconjugated]
(73 Publications)
Species: Hu, Mu
Applications: ChIP, ICC, IP, Simple Western, WB
POMC Antibody
NB100-1533
Flow Cytometry POMC Antibody
Immunohistochemistry POMC Antibody
Immunohistochemistry POMC Antibody
(10 Publications)
Species: Hu, Mu, Po, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
LIF Antibody
(39N7D10) - Azide and ...
NBP2-80834
LIF Antibody (39N7D10) - Azide and BSA Free
Species: Hu, Mu
Applications: WB, ICC/IF, IHC
Supplier Logo

Publications for LIF Antibody (NBP2-80834) (0)

There are no publications for LIF Antibody (NBP2-80834).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for LIF Antibody (NBP2-80834) (0)

There are no reviews for LIF Antibody (NBP2-80834). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LIF Antibody (NBP2-80834) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional LIF Products

Research Areas for LIF Antibody (NBP2-80834)

Find related products by research area.

Blogs on LIF.

Check out the latest blog posts on LIF.
Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.
KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.
mFluor Violet Conjugated Antibodies

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our LIF Antibody (39N7D10) - Azide and BSA Free and receive a gift card or discount.


Bioinformatics

Gene Symbol LIF