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LEF1 Antibody (LEF1/6764)

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Analysis of Protein Array containing more than 19,000 full-length human proteins using LEF1-Monospecific Mouse Monoclonal Antibody (LEF1/6764).
Analysis of Protein Array containing more than 19,000 full-length human proteins using LEF1-Monospecific Mouse Monoclonal Antibody (LEF1/6764).
Formalin-fixed, paraffin-embedded human tonsil stained with LEF1 antibody (LEF1/6764). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human tonsil stained with LEF1 antibody (LEF1/6764). Inset: PBS instead of primary antibody; secondary only negative control.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
LEF1/6764
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-20946AF350
Alexa Fluor 405
NBP3-20946AF405
Alexa Fluor 488
NBP3-20946AF488
Alexa Fluor 532
NBP3-20946AF532
Alexa Fluor 594
NBP3-20946AF594
Alexa Fluor 647
NBP3-20946AF647
Alexa Fluor 700
NBP3-20946AF700
Alexa Fluor 750
NBP3-20946AF750
Allophycocyanin
NBP3-20946APC
Biotin
NBP3-20946B
CoraFluor 1
NBP3-20946CL1
DyLight 350
NBP3-20946UV
DyLight 405
NBP3-20946V
DyLight 488
NBP3-20946G
DyLight 550
NBP3-20946R
DyLight 594
NBP3-20946DL594
DyLight 650
NBP3-20946C
DyLight 680
NBP3-20946FR
DyLight 755
NBP3-20946IR
FITC
NBP3-20946F
HRP
NBP3-20946H
Janelia Fluor 525
NBP3-20946JF525
Janelia Fluor 549
NBP3-20946JF549
Janelia Fluor 646
NBP3-20946JF646
Janelia Fluor 669
NBP3-20946JF669
PE
NBP3-20946PE
PerCP
NBP3-20946PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-20946
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LEF1 Antibody (LEF1/6764) Summary

Description
Positive Controls: Human tonsil and thymus.

Antibody with azide store at 2 to 8C. Antibody without azide store at -20 to -80C. Non-hazardous. No MSDS required.
Immunogen
Recombinant fragment of human LEF1 protein (exact sequence is proprietary)
Localization
Nucleus.
Specificity
Anti-LEF1 may be used as an aid for differentiation of chronic lymphocytic leukemia/small lymphocytic lymphoma from other small B cell lymphomas
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
LEF1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for LEF1 Antibody (LEF1/6764)

  • DKFZp586H0919
  • FLJ46390
  • LEF1
  • LEF-1
  • lymphoid enhancer-binding factor 1
  • T cell-specific transcription factor 1-alpha
  • TCF10
  • TCF1ALPHA
  • TCF1-alpha
  • TCF7L3

Background

Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP3-20278
LEF1 Antibody (LEF1/6764)
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Bioinformatics

Gene Symbol LEF1
Uniprot