LEF1 Antibodies
We offer LEF1 Antibodies for use in common research applications: ELISA, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Sandwich ELISA, Western Blot. Each LEF1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our LEF1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the LEF1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our LEF1 polyclonal antibodies and browse our LEF1 monoclonal antibody catalog.
Alternate Names for LEF1 Antibodies
anti-LEF1 antibody, anti-DKFZp586H0919 antibody, anti-FLJ46390 antibody, anti-LEF-1 antibody, anti-lymphoid enhancer-binding factor 1 antibody, anti-T cell-specific transcription factor 1-alpha antibody, anti-TCF1-alpha antibody, anti-TCF1ALPHA antibody
20 Results for "lef1" in Primary Antibodies
![Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - LEF1 monoclonal antibody (M01), clone 3H5 Analysis of LEF1 expression in MES-SA/Dx5.](http://images.novusbio.com/fullsize/LEF1-Antibody-3H5-Western-Blot-H00051176-M01-img0009.jpg "Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - LEF1 monoclonal antibody (M01), clone 3H5 Analysis of LEF1 expression in MES-SA/Dx5.")
![Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - Analysis of LEF1 over-expressed 293 cell line, cotransfected with LEF1 Validated Chimera RNAi ( Cat # H00051176-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with LEF1 monoclonal antibody (M01), clone 3H5 (Cat # H00051176-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/LEF1-Antibody-3H5-Western-Blot-H00051176-M01-img0006.jpg)
![Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - LEF1 monoclonal antibody (M01), clone 3H5. Analysis of LEF1 expression in HL-60.](http://images.novusbio.com/fullsize/LEF1-Antibody-3H5-Western-Blot-H00051176-M01-img0008.jpg)
![Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - Analysis of LEF1 expression in transfected 293T cell line by LEF1 monoclonal antibody (M01), clone 3H5.Lane 1: LEF1 transfected lysate(44.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/LEF1-Antibody-3H5-Western-Blot-H00051176-M01-img0010.jpg)
![Sandwich ELISA: LEF1 Antibody (3H5) [H00051176-M01] - Detection limit for recombinant GST tagged LEF1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/LEF1-Antibody-3H5-Sandwich-ELISA-H00051176-M01-img0007.jpg)
![Western Blot: LEF1 Antibody [NB100-41374] - Staining of K562 nuclear cell lysate (A) + peptide (B). Antibody at 2 ug/mL. 35 ug protein in RIPA buffer. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/LEF1-Antibody-Western-Blot-NB100-41374-img0001.jpg "Western Blot: LEF1 Antibody [NB100-41374] - Staining of K562 nuclear cell lysate (A) + peptide (B). Antibody at 2 ug/mL. 35 ug protein in RIPA buffer. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: LEF1 Antibody [NB100-41374] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/LEF1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41374-img0003.jpg)
![Flow Cytometry: LEF1 Antibody [NB100-41374] - Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/LEF1-Antibody-Flow-Cytometry-NB100-41374-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: LEF1 Antibody [NB100-41374] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/LEF1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41374-img0002.jpg)
![Western Blot: LEF1 Antibody [NBP1-86960] - Analysis in human cell lines SK-MEL-30 and A-549 using anti-LEF1 antibody. Corresponding LEF1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.](http://images.novusbio.com/fullsize/LEF1-Antibody-Western-Blot-NBP1-86960-img0023.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: LEF1 Antibody [NBP1-86960] - Analysis in human cell lines SK-MEL-30 and A-549 using anti-LEF1 antibody. Corresponding LEF1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: LEF1 Antibody [NBP1-86960] - Analysis in control (vector only transfected HEK293T lysate) and LEF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/LEF1-Antibody-Western-Blot-NBP1-86960-img0025.jpg)
![Immunocytochemistry/Immunofluorescence: LEF1 Antibody [NBP1-86960] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LEF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86960-img0024.jpg)
![Immunohistochemistry-Paraffin: LEF1 Antibody [NBP1-86960] - Staining of human oral mucosa shows moderate nuclear positivity in subset of squamous epithelial cells.](http://images.novusbio.com/fullsize/LEF1-Antibody-Immunohistochemistry-Paraffin-NBP1-86960-img0018.jpg)
![Immunohistochemistry-Paraffin: LEF1 Antibody [NBP1-86960] - Staining of human lymph node shows moderate to strong nuclear positivity in non - germinal center cells.](http://images.novusbio.com/fullsize/LEF1-Antibody-Immunohistochemistry-Paraffin-NBP1-86960-img0017.jpg)
![Western Blot: LEF1 Antibody (JE52-28) [NBP3-32527] - Western blot analysis of LEF1 on different lysates with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (negative) (20 ug/Lane) Lane 3: Mouse thymus tissue lysate (40 ug/Lane) Lane 4: Rat thymus tissue lysate (40 ug/Lane) Predicted band size: 44 kDa Observed band size: 35-55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32527) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32527_rabbit-lef1-mab-je52-28-278202415212459.jpg "Western Blot: LEF1 Antibody (JE52-28) [NBP3-32527] - Western blot analysis of LEF1 on different lysates with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (negative) (20 ug/Lane) Lane 3: Mouse thymus tissue lysate (40 ug/Lane) Lane 4: Rat thymus tissue lysate (40 ug/Lane) Predicted band size: 44 kDa Observed band size: 35-55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32527) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LEF1 Antibody (JE52-28) [NBP3-32527] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32527) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32527_rabbit-lef1-mab-je52-28-298202414333615.jpg)
![Immunocytochemistry/ Immunofluorescence: LEF1 Antibody (JE52-28) [NBP3-32527] - Immunocytochemistry analysis of Jurkat (positive) and HeLa (negative) labeling LEF1 with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32527_rabbit-lef1-mab-je52-28-2920241023422.jpg)
![Western Blot: LEF1 Antibody (5A3) [H00051176-M03] - Analysis of LEF1 expression in HepG2 (Cat # L019V1).](http://images.novusbio.com/fullsize/LEF1-Antibody-5A3-Western-Blot-H00051176-M03-img0007.jpg "Western Blot: LEF1 Antibody (5A3) [H00051176-M03] - Analysis of LEF1 expression in HepG2 (Cat # L019V1).")
![Immunocytochemistry/Immunofluorescence: LEF1 Antibody (5A3) [H00051176-M03] - Analysis of monoclonal antibody to LEF1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/LEF1-Antibody-5A3-Immunocytochemistry-Immunofluorescence-H00051176-M03-img0006.jpg)
![Western Blot: LEF1 Antibody (5A3) [H00051176-M03] - Analysis of LEF1 expression in Jurkat (Cat # L017V1).](http://images.novusbio.com/fullsize/LEF1-Antibody-5A3-Western-Blot-H00051176-M03-img0008.jpg)
![ELISA: LEF1 Antibody (5A3) [H00051176-M03] - Detection limit for recombinant GST tagged LEF1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/LEF1-Antibody-5A3-ELISA-H00051176-M03-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: LEF1 Antibody (REMB6) [NBP2-66597] - Figure 1. Immunofluorescence staining of Cos-1 cells transfected with (top to bottom) full length hLEF1, a LEF1 mutant missing the nuclear localization signal, TCF-4 and TCF -1. Cells were stained with Novus Biologicals' LEF1 antibody, clone REMB6 cat. # T105M and visualized with FITC conjugated mouse IgG. DAPI was used to detect all nuclei. The LEF/TCF/HMG, clone REMB6 recognizes an epitope in the highly conserved HMG DNA binding domain.](http://images.novusbio.com/fullsize/nbp2-66597_mouse-monoclonal-lef1-antibody-remb6-811202420592164.jpg "Immunocytochemistry/ Immunofluorescence: LEF1 Antibody (REMB6) [NBP2-66597] - Figure 1. Immunofluorescence staining of Cos-1 cells transfected with (top to bottom) full length hLEF1, a LEF1 mutant missing the nuclear localization signal, TCF-4 and TCF -1. Cells were stained with Novus Biologicals' LEF1 antibody, clone REMB6 cat. # T105M and visualized with FITC conjugated mouse IgG. DAPI was used to detect all nuclei. The LEF/TCF/HMG, clone REMB6 recognizes an epitope in the highly conserved HMG DNA binding domain.")
![Immunohistochemistry-Paraffin: LEF1 Antibody (LEF1/341R) [NBP3-07769] - IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Strong nuclear staining of non-germinal center cells using LEF1 antibody (LEF1/341R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 : HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/LEF1-Antibody-LEF1-341R-Immunohistochemistry-Paraffin-NBP3-07769-img0002.jpg "Immunohistochemistry-Paraffin: LEF1 Antibody (LEF1/341R) [NBP3-07769] - IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Strong nuclear staining of non-germinal center cells using LEF1 antibody (LEF1/341R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 : HRP-polymer, 30min. DAB, 5min.")
![Immunohistochemistry-Paraffin: LEF1 Antibody (LEF1/341R) [NBP3-07769] - IHC analysis of formalin-fixed, paraffin-embedded human lymph node. Strong nuclear staining of non-germinal center cells using LEF1( LEF1/341R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 : HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/LEF1-Antibody-LEF1-341R-Immunohistochemistry-Paraffin-NBP3-07769-img0001.jpg)
![Immunohistochemistry: LEF1 Antibody (13B7) [NBP3-26171] - Image of LEF1 Antibody (13B7) diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB.](http://images.novusbio.com/fullsize/nbp3-26171_rabbit-lef1-mab-13b7-26220241547562.jpg "Immunohistochemistry: LEF1 Antibody (13B7) [NBP3-26171] - Image of LEF1 Antibody (13B7) diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB.")
![Immunohistochemistry: LEF1 Antibody (13B7) [NBP3-26171] - Image of LEF1 Antibody (13B7) diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB.](http://images.novusbio.com/fullsize/nbp3-26171_rabbit-lef1-mab-13b7-26220241535579.jpg)
![Immunohistochemistry-Paraffin: LEF1 Antibody (rLEF1/6906) [NBP3-13994] - IHC analysis of formalin-fixed, paraffin-embedded human lymph node. Strong nuclear staining of non-germinal center cells using LEF1 antibody (rLEF1/6906) at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary. Secondary antibody negative control.](http://images.novusbio.com/fullsize/LEF1-Antibody-rLEF1-6906-Immunohistochemistry-Paraffin-NBP3-13994-img0001.jpg "Immunohistochemistry-Paraffin: LEF1 Antibody (rLEF1/6906) [NBP3-13994] - IHC analysis of formalin-fixed, paraffin-embedded human lymph node. Strong nuclear staining of non-germinal center cells using LEF1 antibody (rLEF1/6906) at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary. Secondary antibody negative control.")
![Immunohistochemistry-Paraffin: LEF1 Antibody (rLEF1/6906) [NBP3-13994] - IHC analysis of formalin-fixed, paraffin-embedded human lymph node. Strong nuclear staining of non-germinal center cells using LEF1 antibody (rLEF1/6906) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/LEF1-Antibody-rLEF1-6906-Immunohistochemistry-Paraffin-NBP3-13994-img0002.jpg)