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Lamin A + C Antibody

Images

 
Orthogonal Strategies: Western Blot: Lamin A + C Antibody [NBP1-85550] - Lamin A Antibody [NBP1-85550] - Analysis in human cell lines PC-3 and HEK293. Corresponding RNA-seq data are presented for the same cell ...read more
Immunohistochemistry-Paraffin: Lamin A Antibody [NBP1-85550] - Staining of human fallopian tube shows moderate positivity in nuclear membrane in glandular cells.
Immunohistochemistry-Paraffin: Lamin A Antibody [NBP1-85550] - Staining of human prostate shows strong positivity in nuclear membrane in smooth muscle cells and glandular cells.
Immunohistochemistry-Paraffin: Lamin A Antibody [NBP1-85550] - Staining of human stomach shows strong positivity in nuclear membrane in glandular cells.
Immunohistochemistry-Paraffin: Lamin A Antibody [NBP1-85550] - Staining of human skin shows strong positivity in nuclear membrane in squamous epithelial cells.
Western Blot: Rabbit Polyclonal Lamin A + C Antibody [NBP1-85550] - Western blot showing over-expression of lamin A/C tagged with mKate, as well as endogenous lamin A/C, in zebrafish embryos. Actin used as loading ...read more

Product Details

Summary
Reactivity Hu, Ze, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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Lamin A + C Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EVVSREVSGIKAAYEAELGDARKTLDSVAKERARLQLELSKVREEFKELKARNTKKEGDLIAAQARLKDLEALLNSKEAALSTALSEKRTLEGELHDLRGQVAKLEAALGEAKKQLQDEMLRRVDAENRLQTMKEELDFQKNIYSEELRE
Marker
Nuclear Envelope Marker
Predicted Species
Mouse (99%), Rat (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LMNA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Theoretical MW
74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Lamin A Protein (NBP1-85550PEP)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-85550 in the following applications:

Reactivity Notes

Zebrafish reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Lamin A + C Antibody

  • CDCD1
  • CDDC
  • CMD1A
  • CMT2B1
  • dilated 1A (autosomal dominant)
  • EMD2
  • FPLD
  • HGPSFPL
  • lamin A/C
  • lamin A/C-like 1
  • lamin-A/C
  • LDP1
  • LFP
  • LGMD1B
  • limb girdle muscular dystrophy 1B (autosomal dominant)
  • LMN1IDC
  • LMNC
  • LMNL1
  • prelamin-A/C
  • PRO1,70 kDa lamin
  • progeria 1 (Hutchinson-Gilford type)
  • renal carcinoma antigen NY-REN-32

Background

Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lamin A + C Antibody (NBP1-85550) (0)

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Reviews for Lamin A + C Antibody (NBP1-85550) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Zebrafish.

Reviews using NBP1-85550:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Zebrafish
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
João Coelho
WB Zebrafish 11/06/2023
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Summary

ApplicationWestern Blot
Sample Testedzebrafish embryos
SpeciesZebrafish
Lot000025475

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.***
Western Blot Lamin A + C NBP1-85550
Enlarge
4
reviewed by:
Ramanath Narayana Hegde
WB Human 05/21/2015
View

Summary

ApplicationWestern Blot
SpeciesHuman
LotA0104

Product General Protocols

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FAQs for Lamin A + C Antibody (NBP1-85550) (0)

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

João Coelho
11/06/2023
Application: WB
Species: Zebrafish

Ramanath Narayana Hegde
05/21/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol LMNA
Entrez
Uniprot