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LAG-3 Antibody [Unconjugated]

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Western blot shows lysates of mouse liver tissue, mouse heart tissue, EL4.IL-2 mouse lymphoblast cell line, and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse LAG-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3328) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for LAG-3 at approximately 54 kDa and 75 kDa in mouse liver and mouse heart tissue and 70-80 kDa in EL4.IL-2 and CTLL-2 cell lines (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse liver tissue, mouse heart tissue, EL4.IL-2 mouse lymphoblast cell line, and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse LAG-3 ...read more
LAG‑3 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Goat Anti-Mouse LAG‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3328) at 15 µg/mL for 1 ...read more
LAG‑3 was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Mouse LAG‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3328) at 3 µg/mL for 1 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF3328-SP
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LAG-3 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse LAG-3
Gly24-Leu442
Accession # Q61790
Specificity
Detects mouse LAG-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human LAG-3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LAG3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 0.25 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF3328 in the following applications:
Publications
Read Publication using
AF3328 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LAG-3 Antibody [Unconjugated]

  • CD223 antigen
  • CD223
  • LAG3
  • LAG-3
  • lymphocyte activating 3
  • lymphocyte activation gene 3 protein
  • lymphocyte-activation gene 3
  • Secreted lymphocyte activation gene 3 protein
  • sLAG-3

Background

LAG-3 (Lymphocyte activation gene-3; CD223 in the human) is a member of the immunoglobulin superfamily (IgSF). The mature LAG-3 protein is a 496 amino acid (aa) membrane protein with a 421 aa extracellular region which contains four IgSF domains, a 21 aa transmembrane region and a 54 aa cytoplasmic region. LAG-3 shares < 20% amino acid sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity.  The mouse LAG-3 extracellular region shares 69% aa sequence identity with human LAG-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LAG-3 Antibody (AF3328)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: Immunoprecipitation, Western Blot.


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Immunoprecipitation
(1)
Western Blot
(1)
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Mouse
(1)
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Review for LAG-3 Antibody (AF3328) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF3328:
Filter by Applications
WB
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 WB Mouse 01/07/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 22586584
SpeciesMouse

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for LAG-3 Antibody (AF3328). (Showing 1 - 3 of 3 FAQ).

  1. Can you tell me which AA sequence this antibody detects?
    • The exact epitope recognized by this antibody has not yet been mapped. The immunogen for this antibody is aa24-442 of the mouse LAG-3 protein Uniprot.
  2. Can this LAG-3 antibody be used in ELISA? 
    • We have not validated this particular LAG-3 antibody for ELISA in mouse; however, it has been cited as being used as a detection antibody in a publicatoin (PMID 15946792).
  3. I'm using your LAG-3 antibody in western blot but the observed band is a different size than the predicted band size, do you know what the bands are?
    • There are several alternative splice varients of LAG-3 that could explain the different sized proteins stained by the LAG-3 antibody. It is also believed that there are two secreted forms of LAG-3, one that is about 36 kDa and another at 52 kDa.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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 Verified Customer
01/07/2015
Application: WB
Species: Mouse
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Bioinformatics

Gene Symbol LAG3
Uniprot