Home » LAG-3 » LAG-3 Antibodies » LAG-3 Antibody (631501) [Unconjugated]

LAG-3 Antibody (631501) [Unconjugated]

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Mouse splenocytes were treated for 72 hours with 50 ng/mL PMA and 500 ng/mL Ca2+Ionomycin then stained with Rat Anti-Mouse LAG-3 Monoclonal Antibody (Catalog # MAB33281, filled histogram) or isotype control antibody (MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113).
Biological Strategies: Mouse splenocytes were treated for 72 hours with 50 ng/mL PMA and 500 ng/mL Ca2+Ionomycin then stained with Rat Anti-Mouse LAG-3 Monoclonal Antibody (Catalog # MAB33281, filled histogram) ...read more
CTLL‑2 mouse cytotoxic T cell line were stained with Rat Anti-Mouse LAG‑3 Monoclonal Antibody (Catalog # MAB33281, filled histogram) or isotype control antibody (Catalog # MAB0061, open ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
631501
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
   

Biological Strategies

      

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB33281U-100UG
Alexa Fluor 405
FAB33281V-100UG
Alexa Fluor 488
FAB33281G-100UG
Alexa Fluor 532
FAB33281X
Alexa Fluor 594
FAB33281T-100UG
Alexa Fluor 647
FAB33281R-100UG
Alexa Fluor 700
FAB33281N
Alexa Fluor 750
FAB33281S-100UG
Allophycocyanin
FAB33281A
Allophycocyanin/Cy7
FAB33281APCCY7
Biotin
FAB33281B
CoraFluor 1
FAB33281CL1
DyLight 350
FAB33281D
DyLight 405
FAB33281E
DyLight 488
FAB33281K
DyLight 550
FAB33281L
DyLight 594
FAB33281M
DyLight 650
FAB33281W
DyLight 680
FAB33281Y
DyLight 755
FAB33281Z
FITC
FAB33281F
HRP
FAB33281H
Janelia Fluor 525
FAB33281JF525
Janelia Fluor 549
FAB33281I
Janelia Fluor 646
FAB33281J
Janelia Fluor 669
FAB33281JF669
PE/Atto594
FAB33281PEATT594
PE/Cy5.5
FAB33281PECY55
PE/Cy7
FAB33281PECY7
PerCP
FAB33281C
Phycoerythrin
FAB33281P-100UG
mFluor Violet 450 SE
FAB33281MFV450
mFluor Violet 500 SE
FAB33281MFV500
mFluor Violet 610 SE
FAB33281MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB33281N
Carrier Free
MAB33281-SP
Carrier Free
FAB33281V-100UG
Carrier Free
FAB33281U-100UG
Carrier Free
FAB33281T-100UG
Carrier Free
FAB33281S-100UG
Carrier Free
FAB33281R-100UG
Carrier Free
FAB33281P-100UG
Carrier Free
FAB33281G-100UG
Novus Biologicals offers 100% Guarantee

LAG-3 Antibody (631501) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with mouse LAG-3
Ser23-Leu442
Accession # Q61790
Specificity
Detects mouse LAG-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LAG-3 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
LAG3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LAG-3 Antibody (631501) [Unconjugated]

  • CD223 antigen
  • CD223
  • LAG3
  • LAG-3
  • lymphocyte activating 3
  • lymphocyte activation gene 3 protein
  • lymphocyte-activation gene 3
  • Secreted lymphocyte activation gene 3 protein
  • sLAG-3

Background

LAG-3 (Lymphocyte activation gene-3; CD223 in the human) is a member of the immunoglobulin superfamily (IgSF). The mature LAG-3 protein is a 496 amino acid (aa) membrane protein with a 421 aa extracellular region which contains four IgSF domains, a 21 aa transmembrane region and a 54 aa cytoplasmic region. LAG-3 shares < 20% amino acid sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity.  The mouse LAG-3 extracellular region shares 69% aa sequence identity with human LAG-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for LAG-3 Antibody (MAB33281). (Showing 1 - 2 of 2 FAQ).

  1. Can this LAG-3 antibody be used in ELISA? 
    • We have not validated this particular LAG-3 antibody for ELISA in mouse; however, it has been cited as being used as a detection antibody in a publicatoin (PMID 15946792).
  2. I'm using your LAG-3 antibody in western blot but the observed band is a different size than the predicted band size, do you know what the bands are?
    • There are several alternative splice varients of LAG-3 that could explain the different sized proteins stained by the LAG-3 antibody. It is also believed that there are two secreted forms of LAG-3, one that is about 36 kDa and another at 52 kDa.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LAG3
Uniprot