Novus Biologicals products are now on bio-techne.com

LAG-3 Antibody (631501) [Alexa Fluor® 700]

Images

 
Biological Strategies: Mouse splenocytes treated with PMA and Calcium Ionomycin for 3 days were stained with Rat Anti-Mouse LAG-3 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB33281N, filled ...read more
CTLL‑2 mouse cytotoxic T cell line were stained with Rat Anti-Mouse LAG‑3 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB33281N, filled histogram) or isotype control ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
631501
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 700
Validated by:
   

Biological Strategies

     

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

LAG-3 Antibody (631501) [Alexa Fluor® 700] Summary

Immunogen
NS0 mouse myeloma cell line transfected with mouse LAG-3
Ser23-Leu442
Accession # Q61790
Specificity
Detects mouse LAG-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LAG-3 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
LAG3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LAG-3 Antibody (631501) [Alexa Fluor® 700]

  • CD223 antigen
  • CD223
  • LAG3
  • LAG-3
  • lymphocyte activating 3
  • lymphocyte activation gene 3 protein
  • lymphocyte-activation gene 3
  • Secreted lymphocyte activation gene 3 protein
  • sLAG-3

Background

LAG-3 (Lymphocyte activation gene-3; CD223 in the human) is a member of the immunoglobulin superfamily (IgSF). The mature LAG-3 protein is a 496 amino acid (aa) membrane protein with a 421 aa extracellular region which contains four IgSF domains, a 21 aa transmembrane region and a 54 aa cytoplasmic region. LAG-3 shares < 20% amino acid sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity.  The mouse LAG-3 extracellular region shares 69% aa sequence identity with human LAG-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

7268-CT
Species: Hu
Applications: BA
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-67605
Species: Hu, Pm, Mu
Applications: ICC/IF, IHC, IHC-P, IP, ISH, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
NBP1-19371
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
NBP2-29460
Species: Pm, Pm-Cm, Hu, Pm, RM
Applications: Flow, ICC/IF, IHC, IHC-P, ISH
AF796
Species: Mu
Applications: AdBlk, IHC, WB
AF123
Species: Hu
Applications: Block, ICC, WB
6507-IL/CF
Species: Hu
Applications: BA
AF2319
Species: Hu
Applications: CyTOF-reported, Flow, IHC, WB
NBP1-49045
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
AF632
Species: Hu
Applications: AgAct, Simple Western, WB
NB200-540
Species: Ec, Mu
Applications: CyTOF-ready, Flow-IC, Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
211-TBB/CF
Species: Hu
Applications: BA
NB100-56605
Species: Av, Bv, Sh
Applications: WB
AF114
Species: Mu
Applications: CyTOF-ready, Flow, ICC, IHC, WB

Publications for LAG-3 Antibody (FAB33281N) (0)

There are no publications for LAG-3 Antibody (FAB33281N).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for LAG-3 Antibody (FAB33281N) (0)

There are no reviews for LAG-3 Antibody (FAB33281N). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for LAG-3 Antibody (FAB33281N). (Showing 1 - 2 of 2 FAQ).

  1. Can this LAG-3 antibody be used in ELISA? 
    • We have not validated this particular LAG-3 antibody for ELISA in mouse; however, it has been cited as being used as a detection antibody in a publicatoin (PMID 15946792).
  2. I'm using your LAG-3 antibody in western blot but the observed band is a different size than the predicted band size, do you know what the bands are?
    • There are several alternative splice varients of LAG-3 that could explain the different sized proteins stained by the LAG-3 antibody. It is also believed that there are two secreted forms of LAG-3, one that is about 36 kDa and another at 52 kDa.

Secondary Antibodies

 

Isotype Controls

Additional LAG-3 Products

Blogs on LAG-3.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our LAG-3 Antibody (631501) [Alexa Fluor&#174; 700] and receive a gift card or discount.

Bioinformatics

Gene Symbol LAG3
Uniprot