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Lactoferrin Antibody (SR2218)

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Western Blot: Lactoferrin Antibody (SR2218) [NBP3-21725] - Western blot analysis using NBP3-21725 in HeLa cell lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clone
SR2218
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Lactoferrin Antibody (SR2218) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human Lactoferrin (Uniprot #: P02788)
Specificity
Detects endogenous levels of total Lactoferrin
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
LTF
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Lactoferrin Antibody (SR2218)

  • EC 3.4.21
  • EC 3.4.21.-
  • GIG12
  • growth-inhibiting protein 12
  • HEL110
  • HLF2
  • Kaliocin-1
  • Lactoferricin
  • Lactoferrin
  • Lactoferroxin
  • Lactotransferrin
  • LF
  • LTF
  • neutrophil lactoferrin
  • Talalactoferrin

Background

Lactoferrin is an iron binding glycoprotein with an approximate molecular weight of 80 kDa. The protein has two iron binding domains each housing one Fe3+ and the synergistic CO32- ion. The crystal structure form of human lactoferrin at 2.2A resolution exhibits 5330 protein atoms, 2Fe2+, 2CO32- and 98 carbohydrate atoms. Lactoferrin is absorbed from intestine by apical side of the membrane and localized to the nuclei. Intravenous infusion of lactoferrin is protective against lethal doses of E coli and induce bacterimia by a mechanism that downregulates neutrophil TNF alfa secretion. Recombinant human lactoferrin (rhLF), expressed and extracted from rice seed, is being evaluated for use as a dietary supplement to treat iron deficiency and/or iron deficiency induced anemia. Lactoferrin has been shown to have a role in the immune system and in early development of the embryo. A specific receptor for lactoferrin binding has been implicated in the human fetal intestine. Early embryonic localisation of lactoferrin by IHC has suggested its presence in various tissues including intestinal epitheliuem, kiney, and various regions of the brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LTF