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L1CAM Antibody (SPM275) [Janelia Fluor® 669]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC, CyTOF-ready
Clone
SPM275
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 669

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-34753AF350
Alexa Fluor 405
NBP2-34753AF405
Alexa Fluor 488
NBP2-34753AF488
Alexa Fluor 532
NBP2-34753AF532
Alexa Fluor 594
NBP2-34753AF594
Alexa Fluor 647
NBP2-34753AF647
Alexa Fluor 700
NBP2-34753AF700
Alexa Fluor 750
NBP2-34753AF750
Allophycocyanin
NBP2-34753APC
Biotin
NBP2-34753B
CoraFluor 1
NBP2-34753CL1
DyLight 350
NBP2-34753UV
DyLight 405
NBP2-34753V
DyLight 488
NBP2-34753G
DyLight 550
NBP2-34753R
DyLight 594
NBP2-34753DL594
DyLight 650
NBP2-34753C
DyLight 680
NBP2-34753FR
DyLight 755
NBP2-34753IR
FITC
NBP2-34753F
HRP
NBP2-34753H
Janelia Fluor 525
NBP2-34753JF525
Janelia Fluor 549
NBP2-34753JF549
Janelia Fluor 585
NBP2-34753JF585
Janelia Fluor 635
NBP2-34753JF635
Janelia Fluor 646
NBP2-34753JF646
PE
NBP2-34753PE
PerCP
NBP2-34753PCP
Unconjugated
NBP2-33016-0.1mg
mFluor Violet 450 SE
NBP2-34753MFV450
mFluor Violet 500 SE
NBP2-34753MFV500
mFluor Violet 610 SE
NBP2-34753MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-33016-0.1mg
Azide and BSA Free
NBP2-34753-0.1mg
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L1CAM Antibody (SPM275) [Janelia Fluor® 669] Summary

Immunogen
Homogenous suspension of 16-week human fetal brain
Localization
Cell surface
Marker
Axonal Marker
Specificity
Recognizes a cell surface protein of 220-240kDa, identified as L1 cell adhesion molecule. The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, and adducted thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, and neural migration as well as in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm. This monoclonal antibody is useful in the identification of primitive neuroectodermal tumors. It binds to tumors of neuroectodermal and glial origin e.g. neuroblastoma and Schwannomas. It does not bind to pediatric or adult brain.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
L1CAM
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for L1CAM Antibody (SPM275) [Janelia Fluor® 669]

  • antigen identified by monoclonal R1
  • CAML1
  • CAML1N-CAML1
  • CD171 antigen
  • CD171
  • HSAS
  • HSAS1
  • L1 cell adhesion molecule
  • L1CAM
  • MASA
  • MIC5
  • N-CAM-L1
  • NCAM-L1
  • neural cell adhesion molecule L1
  • S10
  • SPG1

Background

The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, adductus thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, neural migration and in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol L1CAM