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L1CAM Antibody (OTI2A6)

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Western Blot: L1CAM Antibody (OTI2A6) [NBP2-02149] - Analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.
Western Blot: L1CAM Antibody (OTI2A6) [NBP2-02149] - Analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY L1CAM.
Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded pancreas tissue using anti-L1CAMmouse monoclonal antibody.
Flow Cytometry: L1CAM Antibody (OTI2A6) [NBP2-02149] - Analysis of Jurkat cells, using anti-L1CAM antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Western Blot: L1CAM Antibody (OTI2A6) [NBP2-02149] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded endometrium tissue within the normal limits using anti-L1CAM mouse monoclonal antibody. Heat-induced epitope retrieval ...read more
Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-L1CAMmouse monoclonal antibody.
Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-L1CAM mouse monoclonal antibody.
Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded breast tissue using anti-L1CAM mouse monoclonal antibody.
Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded colon tissue using anti-L1CAM mouse monoclonal antibody.
Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded Kidney tissue using anti-L1CAM mouse monoclonal antibody.
Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded Ovary tissue using anti-L1CAM mouse monoclonal antibody.
Flow Cytometry: L1CAM Antibody (OTI2A6) [NBP2-02149] - Analysis of Hela cells, using anti-L1CAM antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Immunoprecipitation: L1CAM Antibody (OTI2A6) [NBP2-02149] - Immunoprecipitation of L1CAM by using TrueMab monoclonal anti-L1CAM antibody (Negative control: IP without adding anti-L1CAM antibody). For each experiment, ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clone
OTI2A6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.97 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71097AF350
Alexa Fluor 405
NBP2-71097AF405
Alexa Fluor 488
NBP2-71097AF488
Alexa Fluor 532
NBP2-71097AF532
Alexa Fluor 594
NBP2-71097AF594
Alexa Fluor 647
NBP2-71097AF647
Alexa Fluor 700
NBP2-71097AF700
Alexa Fluor 750
NBP2-71097AF750
Allophycocyanin
NBP2-71097APC
Allophycocyanin/Cy7
NBP2-71097APCCY7
Biotin
NBP2-71097B
CoraFluor 1
NBP2-71097CL1
DyLight 350
NBP2-71097UV
DyLight 405
NBP2-71097V
DyLight 488
NBP2-71097G
DyLight 550
NBP2-71097R
DyLight 594
NBP2-71097DL594
DyLight 650
NBP2-71097C
DyLight 680
NBP2-71097FR
DyLight 755
NBP2-71097IR
FITC
NBP2-71097F
HRP
NBP2-71097H
Janelia Fluor 525
NBP2-71097JF525
Janelia Fluor 549
NBP2-71097JF549
Janelia Fluor 646
NBP2-71097JF646
Janelia Fluor 669
NBP2-71097JF669
PE
NBP2-71097PE
PE/Atto594
NBP2-71097PEATT594
PE/Cy5.5
NBP2-71097PECY55
PE/Cy7
NBP2-71097PECY7
PerCP
NBP2-71097PCP
mFluor Violet 450 SE
NBP2-71097MFV450
mFluor Violet 500 SE
NBP2-71097MFV500
mFluor Violet 610 SE
NBP2-71097MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-71097
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L1CAM Antibody (OTI2A6) Summary

Immunogen
Full length human recombinant protein of human IDH3A (NP_005521) produced in HEK293T cell.
Marker
Axon Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
L1CAM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 2-4ug/mg
  • Western Blot 1:1000
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.97 mg/ml
Purity
Immunogen affinity purified

Alternate Names for L1CAM Antibody (OTI2A6)

  • antigen identified by monoclonal R1
  • CAML1
  • CAML1N-CAML1
  • CD171 antigen
  • CD171
  • HSAS
  • HSAS1
  • L1 cell adhesion molecule
  • L1CAM
  • MASA
  • MIC5
  • N-CAM-L1
  • NCAM-L1
  • neural cell adhesion molecule L1
  • S10
  • SPG1

Background

The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol L1CAM
Entrez