L1CAM Antibody (84307) [Alexa Fluor® 700] Summary
Immunogen |
Mouse myeloma cell line NS0-derived recombinant human L1CAM Met1-Glu1120 Accession # CAA42508 |
Specificity |
Detects human L1CAM in direct ELISAs. |
Source |
N/A |
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Purity Statement |
Protein A or G purified from ascites |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Flow Cytometry 0.25-1 ug/10^6 cells
|
Packaging, Storage & Formulations
Storage |
Protect from light. Do not freeze.- 12 months from date of receipt, 2 to 8 °C as supplied.
|
Buffer |
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. |
Notes
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for L1CAM Antibody (84307) [Alexa Fluor® 700]
Background
L1CAM (Neural cell adhesion molecule L1, also known as L1, CD171 and NCAM-L1) is a 200-230 kDa member of the L1 family, Immunoglobulin (Ig) superfamily of molecules. L1 is recognized to play a key role in cell migration, adhesion, neurite outgrowth, myelination and neuronal differentiation. It does so through a series of cis and trans interactions that involve multiple copartners and target receptors. L1 is described as forming both homotypic and heterotypic complexes, the latter with molecules as diverse as the EGFR, NCAM, CD24, neurocan and various alpha v plus beta 1 and beta 3 integrins. Cells known to express L1 include immature oligodendrocytes, CD4+ T cells, B cells and monocytes, premyelinating Schwann cells, intestinal epithelial progenitor cells, and cerebellar granule plus Purkinje cells. Mature human L1 is a 1238 amino acid (aa) type I transmembrane protein. It contains an 1101 aa extracellular region (aa 20-1120) plus a 114 aa cytoplasmic domain (aa 1144-1257). The extracellular region possesses six C2-type Ig-like domains (aa 35-607) followed by five fibronectin (FN) type III repeats (aa 612-1108). The cytoplasmic tail contains no kinase motifs, but does possess a FIGQY peptide that interacts with ankyrin, and an RSLE sequence that mediates clathrin-associated endocytosis. At least five Ser residues are known to be phosphorylated. There are two splice variants, one each in the intracellular and extracellular domain. A deletion of RSLE (aa 1177-1180) adversely affects endocytosis, while a Leu substitution for aa 26-31 interfers with numerous heterotypic interactions. In general, the full-length L1 molecule is a neuron-associated isoform. L1 is known to undergo proteolysis, either by plasmin or ADAMs. This generates soluble isoforms of varying sizes (140-200 kDa) that retain bioactivity, and which can be incorporated into the surrounding ECM. The membrane fragments (30-80 kDa) undergo further processing, most importantly by gamma -secretase, to generate a soluble 28 kDa intracellular domain. This domain is SUMOylated, and believed to possess an NLS at Lys1147. Upon presumed entry into the nucleus, L1 is posited to activate L1-responsive genes. In the extracellular region, human and mouse L1 share 86% aa sequence identity.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, KO, Simple Western, WB
Species: Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: CyTOF-ready, Flow, IHC, KO, Neut, WB
Species: Hu
Applications: Simple Western, WB
Species: Hu
Applications: ELISA
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, KD, WB
Species: Hu, Mu
Applications: ELISA, IHC, IHC-P, WB
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
Species: Hu, Mu
Applications: Flow, WB
Species: Bv, Ch, Eq, Gp, Hu, Mu, Po, Rb, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
Species: Ca, Hu, Pm, Mu, Pm, Rt
Applications: CyTOF-ready, Flow, WB
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, Simple Western, WB
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
Species: Dr, Hu, Mu
Applications: IP, WB
Publications for L1CAM Antibody (FAB7772N) (0)
There are no publications for L1CAM Antibody (FAB7772N).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for L1CAM Antibody (FAB7772N) (0)
There are no reviews for L1CAM Antibody (FAB7772N).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for L1CAM Antibody (FAB7772N) (0)