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Mouse KRAS ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Standard Curve Range
0.156 - 10 ng/mL
Sensitivity
0.054 ng/mL

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Mouse KRAS ELISA Kit (Colorimetric) Summary

Description
Assay time: 3 hours
Standard Curve Range
0.156 - 10 ng/mL
Sensitivity
0.054 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12
Intra-Assay
%CV < 10
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
KRAS

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A
  2. Assay Diluent B
  3. Detection Reagent A
  4. Detection Reagent B
  5. Instruction manual
  6. Plate sealer for 96 wells
  7. Pre-coated ready to use 96-well strip plate
  8. Standard Diluent
  9. Standard
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (30 x concentrate)

Alternate Names for Mouse KRAS ELISA Kit (Colorimetric)

  • cellular c-Ki-ras2 proto-oncogene
  • c-Ki-ras
  • C-K-RAS
  • GTPase KRas
  • KI-RAS
  • Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog
  • K-Ras 2
  • K-ras p21 protein
  • KRAS1
  • K-RAS2A
  • K-RAS2B
  • KRAS2NS3
  • K-RAS4A
  • K-RAS4B
  • NS
  • oncogene KRAS2
  • PR310 c-K-ras oncogene
  • RASK2c-Kirsten-ras protein
  • transforming protein p21
  • v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog
  • v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Background

KRAS, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol KRAS