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KiSS1R/GPR54 Antibody

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Immunohistochemistry: KiSS1R/GPR54 Antibody [NBP3-38024] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using KiSS1R/GPR54 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval ...read more
Western Blot: KiSS1R/GPR54 Antibody [NBP3-38024] - Western blot analysis of various lysates using KiSS1R/GPR54 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 ...read more
Immunohistochemistry: KiSS1R/GPR54 Antibody [NBP3-38024] - Immunohistochemistry analysis of paraffin-embedded Rat brain using KiSS1R/GPR54 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval ...read more
Immunohistochemistry: KiSS1R/GPR54 Antibody [NBP3-38024] - Immunohistochemistry analysis of paraffin-embedded Human B cell lymphoma using KiSS1R/GPR54 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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KiSS1R/GPR54 Antibody Summary

Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human KiSS1R/GPR54 (NP_115940.2).

Sequence:
SYAAYALKTWAHCMSYSNSALNPLLYAFLGSHFRQAFRRVCPCAPRRPRRPRRPGPSDPAAPHAELLRLGSHPAPARAQKPGSSGLAARGLCVLGEDNAPL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KISS1R
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:100
  • Western Blot 1:500 - 1:1000
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.09% Sodium Azide
Purity
Affinity purified

Alternate Names for KiSS1R/GPR54 Antibody

  • AXOR12
  • AXOR12hypogonadotropin-1
  • G protein-coupled receptor 54
  • GPR54
  • GPR54kiSS-1R
  • G-protein coupled receptor 54
  • G-protein coupled receptor OT7T175
  • HOT7T175
  • Hypogonadotropin-1
  • KISS1 receptor
  • kiSS-1 receptor
  • KiSS1R
  • KiSS-1R
  • Kisspeptins receptor
  • Metastin Receptor
  • OT7T175

Background

GPR54 is a Metastin Receptor. Metastin, also known as KiSS-1, is a metastasis suppressor gene. Mutations in GPR54 have been shown to cause autosomal recessive idiopathic hypogonadotropic hypogonadism in humans and mice, suggesting that GPR54 is a key regulator for the onset of puberty (Seminara et al. 2003). GPR54 has been reported in various human brain regions, placenta, pancreas, and spinal cord, and at lower levels in spleen, peripheral blood leukocytes, testis, lymph node, thymus, small intestine, stomach, lung, and kidney. The receptor is also abundant in tumor tissues. In rat, GPR54 is expressed in liver, intestine, and in the pons, midbrain, thalamus, hypothalamus, hippocampus, amygdala, cortex (including frontal), and striatum. ESTs have been isolated from normal placenta and kidney cancer libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for KiSS1R/GPR54 Antibody (NBP3-38024) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KISS1R