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Goat anti-Mouse Kappa Light Chain Secondary Antibody

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Western Blot: Goat anti- Mouse, Human Kappa Light Chain Secondary Antibody [NB7546] - Analysis in 1. c whole cell lysate, 2. ASPC1 tumor lysate. Image from verified customer review. Image using the HRP format of this ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Goat anti-Mouse Kappa Light Chain Secondary Antibody Summary

Immunogen
Mouse Kappa Light Chain
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with kappa light chains common to all mouse immunoglobulins. No was detected against lambda light chain or non-immunoglobulin serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGKC
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • ELISA 1:1000 - 1:30000
  • Immunocytochemistry/ Immunofluorescence 1:200- 1:2000
  • Immunohistochemistry 1:200- 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
  • Western Blot 1:1000 - 1:30000
Application Notes
ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500
Publications
Read Publications using NB7546.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Goat anti-Mouse Kappa Light Chain Secondary Antibody

  • HCAK1
  • IGKCD
  • immunoglobulin kappa constant
  • Km
  • MGC111575
  • MGC62011
  • MGC72072
  • MGC88770
  • MGC88771
  • MGC88809

Background

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Kappa Light Chain Antibody (NB7546)(7)


Showing Publications 1 - 7 of 7.
Publications using NB7546 Applications Species
Bauer E, Schlederer M, Scheicher R et al. Cooperation of ETV6/RUNX1 and BCL2 enhances immunoglobulin production and accelerates glomerulonephritis in transgenic mice. Oncotarget. [PMID: 26919255]

Details:
Citation using the HRP form of this antibody.
Miyamoto S, Iwamoto R, Furuya A et al. A Novel Anti-Human HB-EGF Monoclonal Antibody with Multiple Anti-Tumor Mechanisms Against Ovarian Cancer Cells. Clinical Cancer Research: An Official Journal of the American Association for Cancer Research. [PMID: 21918176]

Details:
Citation using the FITC form of this antibody.
Saitoh Y, Terada N, Saitoh S et al. Three-dimensional reconstruction of living mouse liver tissues using cryotechniques with confocal laser scanning microscopy. J Electron Microsc (Tokyo) 2010-01-01 [PMID: 20709827]
Papic N, Hunn JP, Pawlowski N et al. Inactive and active states of the interferon-inducible resistance GTPase, Irga6, in vivo. J Biol Chem 2008-11-01 [PMID: 18784077]
Mehenni H, Lin-Marq N, Buchet-Poyau K et al. LKB1 interacts with and phosphorylates PTEN: a functional link between two proteins involved in cancer predisposing syndromes. Hum Mol Genet. [PMID: 15987703]

Details:
Citation using the HRP form of this antibody.
Tomomori-Sato C, Sato S, Parmely TJ et al. A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2. J Biol Chem 2004-02-01 [PMID: 14638676]
Sato S, Tomomori-Sato C, Banks CA et al. A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex. J Biol Chem 2003-12-01 [PMID: 14576168]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol IGKC