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Goat anti-Mouse Kappa Light Chain Secondary Antibody [HRP]

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Western Blot: Goat anti-Mouse Kappa Light Chain Secondary Antibody [HRP] [NB7549] - Image from verified customer review.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
1.0 mg/ml

Goat anti-Mouse Kappa Light Chain Secondary Antibody [HRP] Summary

Immunogen
Mouse Kappa Light Chain
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with kappa light chains common to all mouse immunoglobulins. No was detected against lambda light chain or non-immunoglobulin serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGKC
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • ELISA 1:10000-1:100000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:500
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
  • Western Blot 1:2000 - 1:20000
Readout System
Reviewed Applications
Read 1 Review rated 5
using
NB7549 in the following applications:

Publications
Read Publications using NB7549.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
Phosphate Buffered Saline (PBS) containing 0.2%BSA
Preservative
0.05% Pro-Clean 400
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Goat anti-Mouse Kappa Light Chain Secondary Antibody [HRP]

  • HCAK1
  • IGKCD
  • immunoglobulin kappa constant
  • Km
  • MGC111575
  • MGC62011
  • MGC72072
  • MGC88770
  • MGC88771
  • MGC88809

Background

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Kappa Light Chain Antibody (NB7549)(3)

Review for Kappa Light Chain Antibody (NB7549) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB7549:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Kappa Light Chain NB7549
Enlarge
5
reviewed by:
Ming Lu
WB Human 09/03/2014
View

Summary

ApplicationWestern Blot
Sample TestedHuman pancreatic cancer cell lysates and tumor grow in mouse of same cells
SpeciesHuman
LotA26

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Kappa Light Chain Antibody (NB7549). (Showing 1 - 2 of 2 FAQs).

  1. Are they free or bind light chains?
    • These are antibodies directed against Light Chains.
  2. If possible could you let me know the sequences of the antigens?
    • Unfortunately the sequence of these antibodies is considered proprietary. They are guaranteed to work in all species and applications listed on the data sheet, and the kappa hasn't been shown to cross-react with lambda and vice versa.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Ming Lu
09/03/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol IGKC