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IST1 Antibody

Images

 
Independent Antibodies: Western Blot: IST1 Antibody [NBP2-33720] - Analysis using Anti-IST1 antibody NBP2-33720 (A) shows similar pattern to independent antibody NBP1-87676 (B).
Immunocytochemistry/ Immunofluorescence: IST1 Antibody [NBP2-33720] - Immunofluorescent staining of human cell line MCF7 shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: IST1 Antibody [NBP2-33720] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: IST1 Antibody [NBP2-33720] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: IST1 Antibody [NBP2-33720] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: IST1 Antibody [NBP2-33720] - Staining of human duodenum shows moderate granular cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

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IST1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELYCDLL
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IST1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
IST1 Protein (NBP2-33720PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for IST1 Antibody

  • IST1 homolog
  • increased sodium tolerance 1 homolog (yeast)
  • OLC1

Background

Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvment in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IST1 Antibody (NBP2-33720) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IST1 Antibody (NBP2-33720) (0)

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Secondary Antibodies

 

Isotype Controls

Additional IST1 Products

Array NBP2-33720

Research Areas for IST1 Antibody (NBP2-33720)

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Blogs on IST1

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Contact Information

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Bioinformatics

Gene Symbol IST1
Uniprot