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Integrin beta 2/CD18 Antibody (MEM-48) [Allophycocyanin/Cy7]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
MEM-48
Clonality
Monoclonal
Host
Mouse
Conjugate
Allophycocyanin/Cy7

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB500-379AF350
Alexa Fluor 405
NB500-379AF405
Alexa Fluor 488
NB500-379AF488
Alexa Fluor 532
NB500-379AF532
Alexa Fluor 594
NB500-379AF594
Alexa Fluor 647
NB500-379AF647
Alexa Fluor 700
NB500-379AF700
Alexa Fluor 750
NB500-379AF750
Allophycocyanin
NB500-379APC
Biotin
NBP1-44612
CoraFluor 1
NB500-379CL1
DyLight 350
NB500-379UV
DyLight 405
NB500-379V
DyLight 594
NB500-379DL594
DyLight 680
NB500-379FR
DyLight 755
NB500-379IR
FITC
NB500-380
HRP
NB500-379H
Janelia Fluor 525
NB500-379JF525
Janelia Fluor 549
NB500-379JF549
Janelia Fluor 585
NB500-379JF585
Janelia Fluor 635
NB500-379JF635
Janelia Fluor 646
NB500-379JF646
Janelia Fluor 669
NB500-379JF669
PE
NB500-379PE
PE/Atto594
NB500-379PEATT594
PE/Cy5.5
NB500-379PECY55
PE/Cy7
NB500-379PECY7
PerCP
NB500-379PCP
Unconjugated
NB500-379
mFluor Violet 450 SE
NB500-379MFV450
mFluor Violet 500 SE
NB500-379MFV500
mFluor Violet 610 SE
NB500-379MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB500-379
BSA Free
NB500-379
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Integrin beta 2/CD18 Antibody (MEM-48) [Allophycocyanin/Cy7] Summary

Immunogen
Leukocytes of a patient suffering from a LGL-type leukemia.
Specificity
The antibody MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGB2
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for Integrin beta 2/CD18 Antibody (MEM-48) [Allophycocyanin/Cy7]

  • 959 beta subunit precursor)10
  • CD18 antigen
  • CD18
  • CD18beta 2
  • cell surface adhesion glycoprotein (LFA-1
  • CR3
  • Integrin beta 2
  • integrin beta-2
  • integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit)
  • integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
  • ITGB2
  • LAD
  • LCAMB
  • leukocyte cell adhesion molecule CD18
  • leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
  • LFA-1 beta
  • LFA-1
  • MAC-1 beta
  • MF17
  • MFI7
  • P150
  • p150,95 beta

Background

CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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The beta-2 integrins, a family of four cell surface transmembrane glycoproteins expressed only on leukocytes, include CD11a/CD18, CD11b/CD18, CD11c/CD18, and CD11d/CD18. They consist of a common beta subunit (CD18) and homologous alpha subunits (CD11a...  Read full blog post.
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Bioinformatics

Gene Symbol ITGB2