![Western Blot: Integrin beta 2/CD18 Antibody (MEM-148) [NB500-480] - Fig. 1. Western Blotting analysis of PMA-activated monocytes. MEM-148 recognizes the lower 65 to 70 kDa zone (activation marker) as well as the upper 78 to 96 kDa zone (CD18).](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-MEM-148-Western-Blot-NB500-480-img0002.jpg "Western Blot: Integrin beta 2/CD18 Antibody (MEM-148) [NB500-480] - Fig. 1. Western Blotting analysis of PMA-activated monocytes. MEM-148 recognizes the lower 65 to 70 kDa zone (activation marker) as well as the upper 78 to 96 kDa zone (CD18).")
| Reactivity | Hu, Po(-)Species Glossary |
| Applications | WB, Flow, IP, CyTOF-ready |
| Clone | MEM-148 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Additional Information | Clone MEM-148 was used by HLDA to establish CD designation. |
| Immunogen | Human tissue / cell preparation, Peripheral blood mononuclear cells (NM_000211.3). |
| Specificity | Recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high-affinity state of LFA-1. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ITGB2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For Flow Cytometry: The antibody is excellent marker of activated myeloid cells. Functional: The antibody induces homotypic cell aggregation and high-affinity LFA-1 conformation in resting leukocytes. WB: Use under non-reducing conditions. Purity is > 95% (by SDS-PAGE). This antibody is CyTOF ready. |
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| Theoretical MW | 95 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.4) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Integrin beta 2/CD18 Antibody (NB500-480)Find related products by research area.
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CD11b/CD18 and Neutrophil-Epithelial Interactions as Targets for Anti-Inflammatory Therapies The beta-2 integrins, a family of four cell surface transmembrane glycoproteins expressed only on leukocytes, include CD11a/CD18, CD11b/CD18, CD11c/CD18, and CD11d/CD18. They consist of a common beta subunit (CD18) and homologous alpha subunits (CD11a... Read full blog post. |
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![Flow Cytometry: Integrin beta 2/CD18 Antibody (MEM-148) [NB500-480] - Analysis using the Biotin conjugate of NB500-480. Staining of human peripheral blood monocytes.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-MEM-148-Flow-Cytometry-NB500-480-img0004.jpg "Flow Cytometry: Integrin beta 2/CD18 Antibody (MEM-148) [NB500-480] - Analysis using the Biotin conjugate of NB500-480. Staining of human peripheral blood monocytes.")
![Western Blot: Integrin beta 2/CD18 Antibody (MEM-148) [NB500-480] - Fig. 1. Western Blotting analysis of PMA-activated neutrophils (Fig. 1A) and monocytes (Fig. 1B), using anti-human CD18 (MEM-148). The antibody MEM-148 recognizes the lower 65 to 70 kDa zone (activation marker) as well as the upper 78 to 96 kDa zone (CD18).](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-MEM-148-Western-Blot-NB500-480-img0001.jpg "Western Blot: Integrin beta 2/CD18 Antibody (MEM-148) [NB500-480] - Fig. 1. Western Blotting analysis of PMA-activated neutrophils (Fig. 1A) and monocytes (Fig. 1B), using anti-human CD18 (MEM-148). The antibody MEM-148 recognizes the lower 65 to 70 kDa zone (activation marker) as well as the upper 78 to 96 kDa zone (CD18).")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")