We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?

Name: iNOS Antibody (2D2-B2) [Unconjugated]
Brand: Novus Biologicals
SKU: MAB9502
Rating: 3 (3 reviews)

We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.

iNOS Antibody (2D2-B2) [Unconjugated] (MAB9502): Novus Biologicals

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, RtSpecies Glossary
Applications	WB, Simple Western, IHC
Clone	2D2-B2
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated

Catalog# & Formulation	Size	Price
Carrier Free MAB9502-SP

Immunogen	Recombinant human iNOS Pro781-His798 Accession # P35228
Specificity	Detects human iNOS. By using synthetic peptides, the epitope recognized by this antibody has been mapped to aa 781-798 of human iNOS. The corresponding sequence of mouse iNOS is identical.
Source	N/A
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	NOS2
Purity Statement	Protein A or G purified from ascites
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Reconstitution Instructions	Reconstitute at 0.5 mg/mL in sterile PBS.

iNOS Antibody (2D2-B2) [Unconjugated]

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Publications for iNOS Antibody (MAB9502)(52)

Reviews for iNOS Antibody (MAB9502) (3) 33

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Publications using MAB9502	Applications	Species
Kawada, K;Ishida, T;Morisawa, S;Jobu, K;Higashi, Y;Aizawa, F;Yagi, K;Izawa-Ishizawa, Y;Niimura, T;Abe, S;Goda, M;Miyamura, M;Ishizawa, K; Atractylodes lancea (Thunb.) DC. [Asteraceae] rhizome-derived exosome-like nanoparticles suppress lipopolysaccharide-induced inflammation in murine microglial cells Frontiers in pharmacology 2024-04-26 [PMID: 38738173] (Western Blot, Mouse)	Western Blot	Mouse
Jorge Miguel Alves-Silva, Sónia Pedreiro, Maria Teresa Cruz, Lígia Salgueiro, Artur Figueirinha Exploring the Traditional Uses of Thymbra capitata Infusion in Algarve (Portugal): Anti-Inflammatory, Wound Healing, and Anti-Aging Pharmaceuticals (Basel) 8/23/2023 [PMID: 37765010]
Suvesh Munakarmi, Yamuna Gurau, Juna Shrestha, Prabodh Risal, Ho Sung Park, Geum-Hwa Lee et al. Synergistic Effects of Vitis vinifera L. and Centella asiatica against CCl4-Induced Liver Injury in Mice International Journal of Molecular Sciences 7/9/2023 [PMID: 37511015]
Ou, Y;Yang, Y;Li, X;Zhang, X;Zhao, L;Yang, C;Wu, Y; Arginine metabolism key enzymes affect the prognosis of myelodysplastic syndrome by interfering with macrophage polarization Cancer medicine 2023-06-27 [PMID: 37366304] (ICC, Human)	ICC	Human
Wenyan Fan, Hongming Xu, Chenling Shen, Jia Fang, Xiaoyan Li Nrf2 orchestrates transition from acute to chronic otitis media through inflammatory macrophages Frontiers in Immunology 4/14/2023 [PMID: 37122744]
Jorge M. Alves-Silva, Sónia Pedreiro, Carlos Cavaleiro, Maria Teresa Cruz, Artur Figueirinha, Lígia Salgueiro Effect of Thymbra capitata (L.) Cav. on Inflammation, Senescence and Cell Migration Nutrients 4/17/2023 [PMID: 37111149]
G Moço, C Sousa, A Capitão, SS MacKinnon, AJ Leitão, AF Mendes Synthesis of Carvone Derivatives and In Silico and In Vitro Screening of Anti-Inflammatory Activity in Murine Macrophages International Journal of Molecular Sciences, 2023-01-23;24(3):. 2023-01-23 [PMID: 36768590] (Western Blot, Mouse)	Western Blot	Mouse
Bo-Yoon Park, Dirong Wu, Kyoo-Ri Kwon, Mi-Jin Kim, Tae-Gon Kim, Jun-Ho Lee et al. Implantation and tracing of green fluorescent protein-expressing adipose-derived stem cells in peri-implant capsular fibrosis Stem Cell Research & Therapy 2/8/2023 [PMID: 36750973]
Jorge M. Alves-Silva, Maria José Gonçalves, Ana Silva, Carlos Cavaleiro, Maria Teresa Cruz, Lígia Salgueiro Chemical Profile, Anti-Microbial and Anti-Inflammaging Activities of Santolina rosmarinifolia L. Essential Oil from Portugal Antibiotics (Basel) 1/15/2023 [PMID: 36671380]
B Saravi, Z Li, V Basoli, S Grad, S Häckel, CE Albers, M Alini, H Schmal, P Obid, G Lang In Vitro Characterization of a Tissue Renin-Angiotensin System in Human Nucleus Pulposus Cells Cells, 2022-10-28;11(21):. 2022-10-28 [PMID: 36359814] (Western Blot, Human)	Western Blot	Human
JY Hong, H Kim, J Lee, WJ Jeon, YJ Lee, IH Ha Harpagophytum procumbens Inhibits Iron Overload-Induced Oxidative Stress through Activation of Nrf2 Signaling in a Rat Model of Lumbar Spinal Stenosis Oxidative Medicine and Cellular Longevity, 2022-09-14;2022(0):3472443. 2022-09-14 [PMID: 36160714] (IHC, Rat)	IHC	Rat
T Kondo, H Aoki, Y Otsuka, Y Kawaguchi, Y Waguri-Nag, M Aoyama Insulin-like growth factor 2 promotes osteoclastogenesis increasing inflammatory cytokine levels under hypoxia Journal of pharmacological sciences, 2022-04-16;149(3):93-99. 2022-04-16 [PMID: 35641033] (Western Blot, Mouse)	Western Blot	Mouse
Chen Li, Jiagui Song, Zhengyang Guo, Yueqing Gong, Tengrui Zhang, Jiaqi Huang et al. EZH2 Inhibitors Suppress Colorectal Cancer by Regulating Macrophage Polarization in the Tumor Microenvironment Frontiers in Immunology 4/1/2022 [PMID: 35432300]
Fabian Gather, Irmgard Ihrig-Biedert, Paul Kohlhas, Tamara Krutenko, Michael Peitz, Oliver Brüstle et al. A specific, non-immune system-related isoform of the human inducible nitric oxide synthase is expressed during differentiation of human stem cells into various cell types Cell Communication and Signaling 4/7/2022 [PMID: 35392923]
Lin Ye, Yu Liu, Xuejing Zhu, Tongyue Duan, Chang Wang, Xiao Fu et al. Digital Spatial Profiling of Individual Glomeruli From Patients With Anti-Neutrophil Cytoplasmic Autoantibody-Associated Glomerulonephritis Frontiers in Immunology 3/2/2022 [PMID: 35309370]
YR Hong, EH Lee, KS Park, M Han, KT Kim, J Park Ultrasound stimulation improves inflammatory resolution, neuroprotection, and functional recovery after spinal cord injury Scientific Reports, 2022-03-07;12(1):3636. 2022-03-07 [PMID: 35256617] (Western Blot, Mouse)	Western Blot	Mouse
Monica Zuzarte, Cátia Sousa, Carlos Cavaleiro, Maria Teresa Cruz, Lígia Salgueiro The Anti-Inflammatory Response of Lavandula luisieri and Lavandula pedunculata Essential Oils Plants (Basel) 1/29/2022 [PMID: 35161351]
F Yu, W Tian, J Dong Anagliptin prevents lipopolysaccharide (LPS)- induced inflammation and activation of macrophages International immunopharmacology, 2022-01-16;104(0):108514. 2022-01-16 [PMID: 35045357] (Western Blot, Mouse)	Western Blot	Mouse
W Katagiri, R Takeuchi, N Saito, D Suda, T Kobayashi Migration and phenotype switching of macrophages at early-phase of bone-formation by secretomes from bone marrow derived mesenchymal stem cells using rat calvaria bone defect model Journal of dental sciences, 2021-08-31;17(1):421-429. 2021-08-31 [PMID: 35028066] (IHC, Rat)	IHC	Rat
H Kim, W Jeon, J Hong, J Lee, C Yeo, Y Lee, S Baek, I Ha Gongjin-Dan Enhances Neurite Outgrowth of Cortical Neuron by Ameliorating H2O2-Induced Oxidative Damage via Sirtuin1 Signaling Pathway Nutrients, 2021-11-27;13(12):. 2021-11-27 [PMID: 34959841] (Western Blot, Rat)	Western Blot	Rat
Y Wang, H Jin, Y Wang, Y Yao, C Yang, J Meng, X Tan, Y Nie, L Xue, B Xu, H Zhao, F Wang Sult2b1 deficiency exacerbates ischemic stroke by promoting pro-inflammatory macrophage polarization in mice Theranostics, 2021-11-01;11(20):10074-10090. 2021-11-01 [PMID: 34815805] (Western Blot, Mouse)	Western Blot	Mouse
S Lim, TJ Kim, YJ Kim, C Kim, SB Ko, BS Kim Senolytic Therapy for Cerebral Ischemia-Reperfusion Injury International Journal of Molecular Sciences, 2021-11-04;22(21):. 2021-11-04 [PMID: 34769397] (IHC, Rat)	IHC	Rat
Jacob Thyrsted, Jacob Storgaard, Julia Blay-Cadanet, Alexander Heinz, Anne Laugaard Thielke, Stefania Crotta et al. Influenza A induces lactate formation to inhibit type I IFN in primary human airway epithelium iScience 11/19/2021 [PMID: 34746710]
Heng Zhou, Pengfei Yang, Haining Li, Liying Zhang, Jin Li, Tianyi Zhang et al. Carbon ion radiotherapy boosts anti-tumour immune responses by inhibiting myeloid-derived suppressor cells in melanoma-bearing mice Cell Death Discovery 11/3/2021 [PMID: 34732697]
L Liu, W Shi, X Xiao, X Wu, H Hu, S Yuan, K Liu, Z Liu BCG immunotherapy inhibits cancer progression by promoting the M1 macrophage differentiation of THP?1 cells via the Rb/E2F1 pathway in cervical carcinoma Oncology reports, 2021-09-28;46(5):. 2021-09-28 [PMID: 34581419] (Western Blot, Human)	Western Blot	Human
JY Hong, J Lee, H Kim, WJ Jeon, C Yeo, BR Choi, JE Yoon, JY Shin, JY Kim, IH Ha Neurotherapeutic Potential of Cervus elaphus Sibericus on Axon Regeneration and Growth Cone Reformation after H2O2-Induced Injury in Rat Primary Cortical Neurons Biology, 2021-08-26;10(9):. 2021-08-26 [PMID: 34571710] (ICC, Rat)	ICC	Rat
A Chu, SY Kok, J Tsui, MC Lin, B Aguirre, M Wadehra Epithelial membrane protein 2 (Emp2) modulates innate immune cell population recruitment at the maternal-fetal interface Journal of reproductive immunology, 2021-03-09;145(0):103309. 2021-03-09 [PMID: 33774530] (IHC, Mouse)	IHC	Mouse
C Vaamonde-G, N Flórez-Fer, MD Torres, MJ Lamas-Vázq, FJ Blanco, H Domínguez, R Meijide-Fa Study of fucoidans as natural biomolecules for therapeutical applications in osteoarthritis Carbohydrate polymers, 2021-01-26;258(0):117692. 2021-01-26 [PMID: 33593565] (Western Blot, Human)	Western Blot	Human
A Silva, AS Oliveira, CV Vaz, S Correia, R Ferreira, L Breitenfel, J Martinez-d, R Palmeira-d, CMF Pereira, A Palmeira-d, MT Cruz Anti-inflammatory potential of Portuguese thermal waters Scientific Reports, 2020-12-18;10(1):22313. 2020-12-18 [PMID: 33339881] (Mouse)		Mouse
Xin-Chao Ji, Ya-Jun Shi, Yan Zhang, Ming-Ze Chang, Gang Zhao Reducing Suppressors of Cytokine Signaling-3 (SOCS3) Expression Promotes M2 Macrophage Polarization and Functional Recovery After Intracerebral Hemorrhage Frontiers in Neurology 11/12/2020 [PMID: 33281724]
LC Cheng, YJ Chao, CY Wang, NN Phan, YL Chen, TW Wang, HP Hsu, YJ Lin, YS Shan, MD Lai Cancer-Derived Transforming Growth Factor-beta Modulates Tumor-Associated Macrophages in Ampullary Cancer Onco Targets Ther, 2020-08-03;13(0):7503-7516. 2020-08-03 [PMID: 32821120] (IHC, Human)	IHC	Human
Rahmeh Othman, Simon Berbari, Elvire Vaucher, Réjean Couture Differential Expression of Kinin Receptors in Human Wet and Dry Age-Related Macular Degeneration Retinae Pharmaceuticals (Basel) 6/24/2020 [PMID: 32599742]
WC Liu, PH Chen, LW Chen Supplementation of endogenous Ahr ligands reverses insulin resistance and associated inflammation in an insulin-dependent diabetic mouse model J. Nutr. Biochem., 2020-04-01;83(0):108384. 2020-04-01 [PMID: 32512500] (Western Blot, Mouse)	Western Blot	Mouse
C Sousa, AJ Leitão, BM Neves, F Judas, C Cavaleiro, AF Mendes Standardised comparison of limonene-derived monoterpenes identifies structural determinants of anti-inflammatory activity Sci Rep, 2020-04-29;10(1):7199. 2020-04-29 [PMID: 32350292] (Western Blot, Human)	Western Blot	Human
CP Faria, BM Neves, Á Lourenço, MT Cruz, JD Martins, A Silva, S Pereira, MDC Sousa Giardia lamblia Decreases NF-&kappaB p65RelA Protein Levels and Modulates LPS-Induced Pro-Inflammatory Response in Macrophages Sci Rep, 2020-04-10;10(1):6234. 2020-04-10 [PMID: 32277133] (Western Blot, N/A)	Western Blot	N/A
MAM Simões, DCGA Pinto, BMR Neves, AMS Silva Flavonoid Profile of the Genista tridentata L., a Species Used Traditionally to Treat Inflammatory Processes Molecules, 2020-02-13;25(4):. 2020-02-13 [PMID: 32069907] (Western Blot, Mouse)	Western Blot	Mouse
C Ma, L Wu, L Song, Y He, S Adel Abdo, S Yan, K Sheng, H Wu, J Ran, L Wu The pro-inflammatory effect of NR4A3 in osteoarthritis J. Cell. Mol. Med., 2019-11-07;0(0):. 2019-11-07 [PMID: 31701670] (Western Blot, Rat)	Western Blot	Rat
Carlos Garaicoa‐Pazmino, Tobias Fretwurst, Cristiane H. Squarize, Tord Berglundh, William V. Giannobile, Lena Larsson et al. Characterization of macrophage polarization in periodontal disease Journal of Clinical Periodontology 8/1/2019 [PMID: 31152604]
Jianjun Wang, Yongliang Yao, Qinghui Zhang, Shasha Li, Lijun Tang Inflammatory responses induced by Helicobacter pylori on the carcinogenesis of gastric epithelial GES‑1 cells International Journal of Oncology 4/9/2019 [PMID: 31081048]
W Ren, J Hou, C Yang, H Wang, S Wu, Y Wu, X Zhao, C Lu Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery J. Exp. Clin. Cancer Res., 2019-02-08;38(1):62. 2019-02-08 [PMID: 30736829] (Western Blot, Xenograft)	Western Blot	Xenograft
Rivera P, Fernandez-Arjona MDM, Silva-Pena D et al. Pharmacological blockade of fatty acid amide hydrolase (FAAH) by URB597 improves memory and changes the phenotype of hippocampal microglia despite ethanol exposure Biochem. Pharmacol. 8/8/2018 [PMID: 30098312]
Y Rubtsov, ? Goryunov, ? Romanov, Y Suzdaltsev, G Sharonov, V Tkachuk Molecular Mechanisms of Immunomodulation Properties of Mesenchymal Stromal Cells: A New Insight into the Role of ICAM-1 Stem Cells Int, 2017-06-27;2017(0):6516854. 6/27/2017 [PMID: 28761447]
Jin-Woo Lee, Young Il Kim, Youngchul Kim, Minji Choi, Seoyeon Min, Yong Hoon Joo et al. Grape seed proanthocyanidin inhibits inflammatory responses in hepatic stellate cells by modulating the MAPK, Akt and NF-kappa B signaling pathways International Journal of Molecular Medicine 7/1/2017 [PMID: 28534957]
S Altan, Z O?urtan Dimethyl sulfoxide but not indomethacin is efficient for healing in hydrofluoric acid eye burns Burns, 2016-10-27;43(1):232-244. 2016-10-27 [PMID: 28341026] (IHC, Rabbit)	IHC	Rabbit
Yasuda Y, Fujita M, Koike E, Obata K, Shiota M, Kotani Y, Musha T, Tsuji-Kawahara S, Satou T, Masuda S, Okano J, Yamasaki H, Okumoto K, Uesugi T, Nakao S, Hoshiai H, Mandai M Erythropoietin Receptor Antagonist Suppressed Ectopic Hemoglobin Synthesis in Xenografts of HeLa Cells to Promote Their Destruction. PLoS ONE, 2015-04-15;10(4):e0122458. 2015-04-15 [PMID: 25874769] (IHC, Human)	IHC	Human
Xia N, Pautz A, Wollscheid U, Reifenberg G, Forstermann U, Li H Artichoke, cynarin and cyanidin downregulate the expression of inducible nitric oxide synthase in human coronary smooth muscle cells. Molecules, 2014-03-24;19(3):3654-68. 2014-03-24 [PMID: 24662080] (Western Blot, Human)	Western Blot	Human
Dietrich S, Okun J, Schmidt K, Falk C, Wagner A, Karamustafa S, Radujkovic A, Hegenbart U, Ho A, Dreger P, Luft T High pre-transplant serum nitrate levels predict risk of acute steroid-refractory graft-versus-host disease in the absence of statin therapy. Haematologica, 2013-10-18;99(3):541-7. 2013-10-18 [PMID: 24142995] (Western Blot, Human)	Western Blot	Human
Parvu AE, Alb SF, Craciun A et al. Efficacy of subantimicrobial-dose doxycycline against nitrosative stress in chronic periodontitis. Acta Pharmacol Sin 11/12/2012 [PMID: 23147558]
Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation. BMC Neurosci, 2012-05-23;13(0):50. 2012-05-23 [PMID: 22621398] (Western Blot, Rat)	Western Blot	Rat
Espinosa A, Oke V, Elfving A, Nyberg F, Covacu R, Wahren-Herlenius M The autoantigen Ro52 is an E3 ligase resident in the cytoplasm but enters the nucleus upon cellular exposure to nitric oxide. Exp. Cell Res., 2008-09-25;314(20):3605-13. 2008-09-25 [PMID: 18845142] (ICC, IHC-P, Human)	ICC, IHC-P	Human
Skaleric U, Gaspirc B, McCartney-Francis N, Masera A, Wahl SM Proinflammatory and antimicrobial nitric oxide in gingival fluid of diabetic patients with periodontal disease. Infect. Immun., 2006-10-02;74(12):7010-3. 2006-10-02 [PMID: 17015454] (IHC-P, Human)	IHC-P	Human
Yuan LJ, Ueng SW, Lin SS, Yeh WL, Yang CY, Lin PY Attenuation of apoptosis and enhancement of proteoglycan synthesis in rabbit cartilage defects by hyperbaric oxygen treatment are related to the suppression of nitric oxide production. J. Orthop. Res., 2004-09-01;22(5):1126-34. 2004-09-01 [PMID: 15304289] (IHC-P, Rabbit)	IHC-P	Rabbit
Show All 52 Publications. Collapse Publications.

	Lenka Uherková 02/12/2020
Application:	Simple Western
Species:	Rat

	Verified Customer 02/25/2015
Application:	WB
Species:	Mouse

Gene Symbol	NOS2
Uniprot	P35228()