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Indoleamine 2,3-dioxygenase/IDO Antibody

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Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP2-80106] - analysis of mouse IDO using anti-IDO (mouse), pAb at 1:2,000 dilution. 1. Recombinant mouse IDO (His-tagged). 2. Con-A treated mouse peripheral blood ...read more
Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP2-80106] - Staining of mouse gut using anti-IDO (mouse), pAb. Colorization by ABS Reagent (Vectastain) and counterstaining with hematoxylin.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

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Indoleamine 2,3-dioxygenase/IDO Antibody Summary

Immunogen
Recombinant mouse Indoleamine 2,3-dioxygenase/IDO.
Specificity
Recognizes mouse Indoleamine 2,3-dioxygenase/IDO. Detects a band of ~45kDa by Western blot.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IDO1
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:2000 - 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:500 - 1:2000
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1:2000 - 1:5000
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 um-filtered solution in PBS, pH 7.4
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Indoleamine 2,3-dioxygenase/IDO Antibody

  • 3dioxygenase
  • EC 1.13.11.52
  • IDO
  • IDO1
  • IDOIDO-1
  • INDO
  • INDOindole 2,3-dioxygenase
  • Indoleamine 2
  • indoleamine 2,3-dioxygenase 1
  • Indoleamine 2,3-dioxygenase
  • indoleamine-pyrrole 2,3 dioxygenase
  • Indoleamine-pyrrole 2,3-dioxygenase

Background

Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.42). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IDO1