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Indoleamine 2,3-dioxygenase/IDO Antibody

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Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87701] - Analysis in control (vector only transfected HEK293T lysate) and IDO1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in ...read more
Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87701] - Staining of human lymph node shows strong cytoplasmic positivity in lymphoid cells.
Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87701] - Staining of human cerebral cortex shows no positivity in neurons as expected.
Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87701] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in lymphoid cells.
Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87701] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in lymphoid cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Indoleamine 2,3-dioxygenase/IDO Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYEGFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMPPAH
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IDO1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Indoleamine 2,3-dioxygenase/IDO Protein (NBP1-87701PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Indoleamine 2,3-dioxygenase/IDO Antibody

  • 3dioxygenase
  • EC 1.13.11.52
  • IDO
  • IDO1
  • IDOIDO-1
  • INDO
  • INDOindole 2,3-dioxygenase
  • Indoleamine 2
  • indoleamine 2,3-dioxygenase 1
  • Indoleamine 2,3-dioxygenase
  • indoleamine-pyrrole 2,3 dioxygenase
  • Indoleamine-pyrrole 2,3-dioxygenase

Background

Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.42). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IDO1