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ILK Antibody

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Western Blot: ILK Antibody [NB100-60451] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. NB100-60451 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.
Western Blot: ILK Antibody [NB100-60451] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. NB100-60451 used for WB at 0.04 ug/ml ...read more
Immunocytochemistry/ Immunofluorescence: ILK Antibody [NB100-60451] - Acetone-fixed asynchronous HeLa cells. Antibody used at a dilution of 1:100 (2ug/ml).

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Gp, Pm-OrSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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ILK Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 402 and 451 of human integrin-linked kinase using the numbering given in entry NP_004508.1 (GeneID 3611).
Predicted Species
Rat (100%), Bovine (100%), Orangutan (100%), Guinea Pig (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ILK
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Acetone fixation is recommended. Permeabilization is not recommended. Formaldehyde fixation is not recommended.
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
ILK Overexpression Lysate
Publications
Read Publication using NB100-60451.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ILK Antibody

  • 59 kDa serine/threonine-protein kinase
  • DKFZp686F1765
  • EC 2.7.11.1
  • ESTM24
  • ILK
  • ILK1
  • ILK-1
  • ILK2
  • ILK-2
  • integrin-linked kinase
  • integrin-linked kinase-2
  • integrin-linked protein kinase
  • p59
  • p59ILK

Background

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ILK Antibody (NB100-60451)(1)

We have publications tested in 1 confirmed species: Mouse.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ILK Antibody (NB100-60451) (0)

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Control Lysate(s)

ILK Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Research Areas for ILK Antibody (NB100-60451)

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Bioinformatics

Gene Symbol ILK
Entrez
Uniprot