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IL18R1 Antibody

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Immunocytochemistry/Immunofluorescence: IL18R1 Antibody [NBP1-85782] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: IL18R1 Antibody [NBP1-85782] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: IL18R1 Antibody [NBP1-85782] - Staining of human lymph node shows moderate to strong membranous positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: IL18R1 Antibody [NBP1-85782] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: IL18R1 Antibody [NBP1-85782] - Staining of human placenta shows strong membranous and cytoplasmic postivity in trhphoblastic cells.
Immunohistochemistry-Paraffin: IL18R1 Antibody [NBP1-85782] - Staining of human rectum shows moderate to strong membranous and cytoplasmic postivity in glandular and lymphoid cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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IL18R1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: HNGKLFNITKTFNITIVEDRSNIVPVLLGPKLNHVAVELGKNVRLNCSALLNEEDVIYWMFGEENGSDPNIHEEKEMRIMTPEGKWHASKVLRIENIGESNLNVLYNCTVASTGGTDTKSFILVRKA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IL18R1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
IL18R1 Protein (NBP1-85782PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for IL18R1 Antibody

  • CD218 antigen-like family member A
  • CD218a antigen
  • CD218a
  • CDw218a
  • cytokine receptor
  • IL-18R1
  • IL-18R-1
  • IL1R-rp
  • IL1RRPIL1 receptor-related protein
  • IL-1RrpIL18RA
  • interleukin 18 receptor 1
  • interleukin-18 receptor 1

Background

IL-18 receptor is composed of an alpha and a beta subunit that combine to form a high affinity receptor for IL-18. IL-18 receptor alpha chain, also known as CDw218a, is a 75-80 kD type I transmembrane protein. It is expressed on NK cells, neutrophils, endothelial cells, and subsets of T and B cells. The expression of CDw218a on lymphocytes is upregulated after activation. The interaction of IL-18 and IL-18 receptor has been reported to be implicated in promotion of Th1 cytokine production and atherogenesis. The H44 antibody is specific for IL-18 receptor alpha chain and has been reported to block IL-18 binding to the receptor and to be useful for the identification of IL-18 receptor by flow cytometry and IHC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IL18R1 Antibody
NBP1-85782
IL18R1 Antibody
Species: Hu
Applications: ICC/IF, IHC

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Video Protocols

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Bioinformatics

Gene Symbol IL18R1