Home » IL-18R alpha/IL-1 R5 » IL-18R alpha/IL-1 R5 Antibodies » IL-18R alpha/IL-1 R5 Antibody [Unconjugated]

IL-18R alpha/IL-1 R5 Antibody [Unconjugated]

 (11 Publications)
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In the presence of Recombinant Mouse IL‑12 (0.1 ng/mL, Catalog # 419-ML), Recombinant Mouse IL‑18/IL‑1F4 stimulates IFN‑ gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN‑ gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN‑ gamma secretion elicited by Recombinant Mouse IL‑18/IL‑1F4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑18 R alpha /IL‑1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF856). The ND50 is typically 0.2-0.8 µg/mL.
In the presence of Recombinant Mouse IL‑12 (0.1 ng/mL, Catalog # 419-ML), Recombinant Mouse IL‑18/IL‑1F4 stimulates IFN‑ gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as ...read more
B6 splenocytes were stained with Rat Anti-Mouse CD3 PE‑conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Goat Anti-Mouse IL‑18 R alpha /IL‑1 R5 Antigen ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF856
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Catalog# & Formulation Size Price
Carrier Free
AF856-SP
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IL-18R alpha/IL-1 R5 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 R alpha /IL-1 R5
Ser19-Gly326
Accession # Q61098
Specificity
Detects mouse IL‑18 R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human IL‑18 R is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
Il18rap
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 0.2-0.8 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF856 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-18R alpha/IL-1 R5 Antibody [Unconjugated]

  • CD218a
  • CD218a;CDw218a;IL18R1;IL18RA;IL-18Ralpha;IL-18R-alpha;IL18Ralpha2;IL1RRP;IL-1Rrp;Interleukin-18 receptor 1
  • IL-1 R5
  • IL-1 Rrp
  • IL-18 R alpha
  • IL-18 R1
  • IL18R alpha
  • IL18R1
  • IL-18Ra

Background

Interleukin-18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immuno-regulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL18 in vitro, coexpression of IL-18 R alpha and IL18 R beta is required for high affinity binding and IL-18 responsiveness.

Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa residues. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IL-18R alpha/IL-1 R5 Antibody [Unconjugated]
Species: Mu
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Publications for IL-18R alpha/IL-1 R5 Antibody (AF856)(11)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: Flow Cytometry, IHC, IHC-Fr, In Vivo, Neutralization.


Filter By Application
Flow Cytometry
(3)
IHC
(2)
IHC-Fr
(2)
In Vivo
(1)
Neutralization
(3)
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Mouse
(10)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF856 Applications Species
Xian Zhang, Songyuan Luo, Minjie Wang, Qin Huang, Wenqian Fang, Jie Li et al. IL18 signaling causes islet beta cell development and insulin secretion via different receptors on acinar and beta cells Developmental Cell 6/6/2022 [PMID: 35675813]
JE Howard, JNP Smith, G Fredman, KC MacNamara IL-18R-mediated HSC quiescence and MLKL-dependent cell death limit hematopoiesis during infection-induced shock Stem Cell Reports, 2021-11-18;0(0):. 2021-11-18 [PMID: 34798063] (In Vivo, Mouse) In Vivo Mouse
T Hayakawa, M Hata, S Kuwahara-O, H Yagi, H Okamura Postnatal changes of interleukin-18 receptor immunoreactivity in neurons of the retrosplenial cortex in wild-type and interleukin-18 knock out mice Okajimas Folia Anat Jpn, 2017-01-01;94(3):93-99. 2017-01-01 [PMID: 29681594] (IHC, Mouse) IHC Mouse
S Kuwahara-O, S Maeda, K Kobayashi, Y Minato, K Tanaka, K Yamanishi, M Hata, W Li, T Hayakawa, K Noguchi, H Okamura, H Yagi Interleukin-18 and its receptor are expressed in gonadotropin-releasing hormone neurons of mouse and rat forebrain Neurosci. Lett., 2017-04-01;0(0):. 2017-04-01 [PMID: 28373090] (IHC, Mouse) IHC Mouse
Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS). J. Biol. Chem., 2009-07-10;0(0):. 2009-07-10 [PMID: 19592492] (Neutralization, Mouse) Neutralization Mouse
Wiener Z, Pocza P, Racz M, Nagy G, Tolgyesi G, Molnar V, Jaeger J, Buzas E, Gorbe E, Papp Z, Rigo J, Falus A IL-18 induces a marked gene expression profile change and increased Ccl1 (I-309) production in mouse mucosal mast cell homologs. Int. Immunol., 2008-10-21;20(12):1565-73. 2008-10-21 [PMID: 18940934] (Flow Cytometry, Neutralization, Mouse) Flow Cytometry, Neutralization Mouse
Andoh T, Kishi H, Motoki K, Nakanishi K, Kuraishi Y, Muraguchi A Protective effect of IL-18 on kainate- and IL-1beta-induced cerebellar ataxia in mice. J. Immunol., 2008-02-15;180(4):2322-8. 2008-02-15 [PMID: 18250441] (IHC-Fr, Mouse) IHC-Fr Mouse
Chandrasekar B, Patel DN, Mummidi S, Kim JW, Clark RA, Valente AJ Interleukin-18 suppresses adiponectin expression in 3T3-L1 adipocytes via a novel signal transduction pathway involving ERK1/2-dependent NFATc4 phosphorylation. J. Biol. Chem., 2007-12-17;283(7):4200-9. 2007-12-17 [PMID: 18086672] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Chaudhry UI, Kingham TP, Plitas G, Katz SC, Raab JR, DeMatteo RP Combined stimulation with interleukin-18 and CpG induces murine natural killer dendritic cells to produce IFN-gamma and inhibit tumor growth. Cancer Res., 2006-11-01;66(21):10497-504. 2006-11-01 [PMID: 17079471] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Wang X, Hagberg H, Mallard C, Zhu C, Hedtjarn M, Tiger CF, Eriksson K, Rosen A, Jacobsson B Disruption of interleukin-18, but not interleukin-1, increases vulnerability to preterm delivery and fetal mortality after intrauterine inflammation. Am. J. Pathol., 2006-09-01;169(3):967-76. 2006-09-01 [PMID: 16936270] (IHC-Fr, Mouse) IHC-Fr Mouse
Show All 11 Publications.

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Bioinformatics

Gene Symbol Il18rap
Uniprot