IL-6 Antibody [Unconjugated]

Images

 
IL-6 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Goat Anti-Human IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA) at 10 ...read more
Recombinant Human IL-6 (Catalog # 206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-6 (2.5 ng/mL) ...read more
Breast DCIS cells overexpress proinflammatory markers. Representative images of immunohistochemistry targeting IL-6 protein in normal breast tissue (a), and breast DCIS (b) (N = 61). c and d Hematoxylin staining of ...read more
IL-6 neutralizing antibody (nAb) inhibits growth of MCF10.DCIS structures. Representative contiguous 16-tiled DIC images of MCF10.DCIS cells grown in MAME cultures for 8 days. MAME culture of MCF10.DCIS cells treated ...read more
IL-6 nAb inhibits CAF-stimulated MCF10.DCIS structure growth and results in altered morphology. Contiguous 16-tile DIC image of CAFs: WS12Ti (A, B) or CAF40TKi (c, d) co-cultured with MCF10.DCIS cells for 8 days in the ...read more
IL-6 neutralizing antibody (nAb) inhibits growth of MCF10.DCIS structures. Representative contiguous 16-tiled DIC images of MCF10.DCIS cells grown in MAME cultures for 8 days. MAME culture of MCF10.DCIS cells treated ...read more
IL-6 nAb inhibits CAF-stimulated MCF10.DCIS structure growth and results in altered morphology. Contiguous 16-tile DIC image of CAFs: WS12Ti (A, B) or CAF40TKi (c, d) co-cultured with MCF10.DCIS cells for 8 days in the ...read more
Breast DCIS cells overexpress proinflammatory markers. Representative images of immunohistochemistry targeting IL-6 protein in normal breast tissue (a), and breast DCIS (b) (N = 61). c and d Hematoxylin staining of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

IL-6 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IL-6
Pro29-Met212
Accession # P05231
Specificity
Detects human IL-6 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IL-6 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL6
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 15-45 ng/mL
Reviewed Applications
Read 4 Reviews rated 4.3
using
AF-206-NA in the following applications:

Publications
Read Publications using
AF-206-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-6 Antibody [Unconjugated]

  • B-cell differentiation factor
  • B-cell stimulatory factor 2
  • BSF2
  • BSF-2
  • CDF
  • CTL differentiation factor
  • HSF
  • hybridoma growth factor
  • IFNB2
  • IFN-beta-2
  • IL6
  • IL-6
  • Interferon beta-2
  • interleukin 6 (interferon, beta 2)
  • interleukin BSF-2
  • interleukin-6
  • MGI-2A

Background

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length expressed as a 22-28 kDA molecular weight protein. IL-6 shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF-206-NA
Species: Hu
Applications: B/N, ICC/IF

Publications for IL-6 Antibody (AF-206-NA)(52)

We have publications tested in 3 confirmed species: Human, N/A, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 13 applications: Affinity Assay, Bioassay, ELISA Capture, ELISA Detection, ELISA Development, Flow Cytometry, Functional Assay, ICC, IHC, IHC-P, Neutralization, Proximity Ligation Assay (PLA), Western Blot.


Filter By Application
Affinity Assay
(1)
Bioassay
(2)
ELISA Capture
(1)
ELISA Detection
(1)
ELISA Development
(1)
Flow Cytometry
(1)
Functional Assay
(1)
ICC
(7)
IHC
(6)
IHC-P
(2)
Neutralization
(16)
Proximity Ligation Assay (PLA)
(1)
Western Blot
(7)
All Applications
Filter By Species
Human
(36)
N/A
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
All Species
Showing Publications 1 - 10 of 52. Show All 52 Publications.
Publications using AF-206-NA Applications Species
Sinclair, JE;Vedelago, C;Ryan, FJ;Carney, M;Redd, MA;Lynn, MA;Grubor-Bauk, B;Cao, Y;Henders, AK;Chew, KY;Gilroy, D;Greaves, K;Labzin, L;Ziser, L;Ronacher, K;Wallace, LM;Zhang, Y;Macauslane, K;Ellis, DJ;Rao, S;Burr, L;Bain, A;Karawita, A;Schulz, BL;Li, J;Lynn, DJ;Palpant, N;Wuethrich, A;Trau, M;Short, KR; Post-acute sequelae of SARS-CoV-2 cardiovascular symptoms are associated with trace-level cytokines that affect cardiomyocyte function Nature microbiology 2024-10-30 [PMID: 39478108] (Bioassay, Human) Bioassay Human
Yan, M;Chen, X;Ye, Q;Li, H;Zhang, L;Wang, Y; IL-33-dependent NF-?B activation inhibits apoptosis and drives chemoresistance in acute myeloid leukemia Cytokine 2024-06-08 [PMID: 38852492] (Western Blot, Human) Western Blot Human
Ozmen, A;Nwabuobi, C;Tang, Z;Guo, X;Larsen, K;Guller, S;Blas, J;Moore, M;Kayisli, UA;Lockwood, CJ;Guzeloglu-Kayisli, O; Leptin-Mediated Induction of IL-6 Expression in Hofbauer Cells Contributes to Preeclampsia Pathogenesis International journal of molecular sciences 2023-12-21 [PMID: 38203306] (IHC, Human) IHC Human
Urban, L;Novák, Š;?oma, M;Dvo?ánková, B;Lacina, L;Šáchová, J;Hradilová, M;Svato?ová, P;Kolá?, M;Strnad, H;B?ezinová, J;Smetana, K;Gál, P;Szabo, P; Unravelling heterogeneous effects of cancer?associated fibroblasts on poor prognosis markers in breast cancer EM?G3 cell line: In vitro?targeted treatment (anti?IL-6, anti?VEGF-A, anti?MFGE8) based on transcriptomic profiling Oncology reports 2024-01-01 [PMID: 37975220] (Neutralization, Human) Neutralization Human
Yang, JL;Lin, WL;Tai, SB;Ciou, YS;Chung, CL;Chen, JJ;Liu, PF;Lin, MW;Chen, CL; Suppression of TGF beta -Induced Interleukin-6 Secretion by Sinulariolide from Soft Corals through Attenuation of the p38-NF-kB Pathway in Carcinoma Cells International journal of molecular sciences 2023-07-19 [PMID: 37511415] (Western Blot, Human) Western Blot Human
Newman, S;Wilson, B;Mamerow, D;Wollant, B;Nyein, H;Rosenberg-Hasson, Y;Maecker, H;Eisenstein, M;Soh, H; Extending the dynamic range of biomarker quantification through molecular equalization bioRxiv 2023-01-01 [PMID: 37443317] (Bioassay, Human) Bioassay Human
W Zeng, L Xiong, W Wu, S Li, J Liu, L Yang, L Lao, P Huang, M Zhang, H Chen, N Miao, Z Lin, Z Liu, X Yang, J Wang, P Wang, E Song, Y Yao, Y Nie, J Chen, D Huang CCL18 signaling from tumor-associated macrophages activates fibroblasts to adopt a chemoresistance-inducing phenotype Oncogene, 2022-11-22;0(0):. 2022-11-22 [PMID: 36418470] (Flow Cytometry, Neutralization, Human) Flow Cytometry, Neutralization Human
YP Sun, YY Lu, J Chen, JH Bao, H Zhang, JD Sun, WT Liao Effects of interleukin 1beta on long noncoding RNA and mRNA expression profiles of human synovial fluid derived mesenchymal stem cells Scientific Reports, 2022-05-19;12(1):8432. 2022-05-19 [PMID: 35589865] (Western Blot, Human) Western Blot Human
JFS Pereira, C Bessa, P Matos, P Jordan Pro-Inflammatory Cytokines Trigger the Overexpression of Tumour-Related Splice Variant RAC1B in Polarized Colorectal Cells Cancers, 2022-03-09;14(6):. 2022-03-09 [PMID: 35326545] (Neutralization, Human) Neutralization Human
Lotta Wik, Niklas Nordberg, John Broberg, Johan Björkesten, Erika Assarsson, Sara Henriksson et al. Proximity Extension Assay in Combination with Next-Generation Sequencing for High-throughput Proteome-wide Analysis Molecular & Cellular Proteomics 2021-10-27 [PMID: 34715355]
Show All 52 Publications.

Reviews for IL-6 Antibody (AF-206-NA) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Human.

Reviews using AF-206-NA:
Filter by Applications
WB
(3)
IP
(1)
All Applications
Filter by Species
Human
(4)
All Species
Images Ratings Applications Species Date Details
Western Blot IL-6 AF-206-NA
Enlarge
4
reviewed by:
Verified Customer
WB Human 12/22/2016
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysates
SpeciesHuman
  4
reviewed by:
Verified Customer
WB Human 01/06/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21784845
SpeciesHuman
  4
reviewed by:
Verified Customer
IP Human 12/19/2014
View

Summary

ApplicationImmunoprecipitation
Sample TestedHEK293 cells transfected with hIL6
SpeciesHuman
  5
reviewed by:
Verified Customer
WB Human 12/19/2014
View

Summary

ApplicationWestern Blot
Sample TestedhIL6 transfected cells
SpeciesHuman

FAQs for IL-6 Antibody (AF-206-NA). (Showing 1 - 1 of 1 FAQs).

  1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
    • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

Secondary Antibodies

 

Isotype Controls

Additional IL-6 Products

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Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.

Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein ...  Read full blog post.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

VEGF: Vascular endothelial growth factor
VEGF is homodimeric, disulfide-linked glycoprotein cytokine that serves as the ligand for FLT1 (VEGFR-1 receptor) and FLK1 (VEGFR-2 receptor) tyrosine kinases. It is a key modulator of physiological angiogenesis, vasculogenesis, and endothelial cell g...  Read full blog post.

TLR2 - I can recognize many foreign pathogens!
TLR2 is a member of the broad family of Toll-like receptors (TLR) that play an important role in pathogen recognition and innate immunity. TLRs, like other pattern recognition receptors (PRRs), recognize endogenous ligands released during cell or tiss...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

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Recent Reviews

4.3
5
1
4
3
3
0
2
0
1
0

Verified Customer
12/22/2016
Application: WB
Species: Human

 
Verified Customer
01/06/2015
Application: WB
Species: Human

 
Verified Customer
12/19/2014
Application: IP
Species: Human

Bioinformatics

Gene Symbol IL6
Uniprot