Home » IL-23R » IL-23R Lysates » IL-23R Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

IL-23R Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: IL23 Receptor Overexpression Lysate (Adult Normal) [NBL1-11944] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL23 Receptor.
Western Blot: IL23 Receptor Overexpression Lysate (Adult Normal) [NBL1-11944] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL23 Receptor.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

IL-23R Overexpression Lysate Summary

Description

IL-23R Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC211477

Accession#: NM_144701

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
IL23R

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for IL-23R Overexpression Lysate

  • IBD17
  • IL-23 R
  • IL-23 receptor
  • IL23R
  • IL-23R
  • interleukin 23 receptor
  • interleukin-23 receptor

Background

IL23 Receptor is encoded by this gene is a subunit of the receptor for IL23A/IL23. This protein pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. This protein associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3 in a ligand-dependent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
IL-23A/IL-23 P19
[Unconjugated]
1290-IL
SEC-MALS IL-23A/IL-23 P19 [Unconjugated]
(115 Publications)
Species: Hu
Applications: BA
IL-17/IL-17A
[Biotin]
DY421
N/A IL-17/IL-17A [Biotin]
(191 Publications)
Species: Mu
Applications: ELISA
IL-37/IL-1F7
[Biotin]
DY1975
N/A IL-37/IL-1F7 [Biotin]
(13 Publications)
Species: Hu
Applications: ELISA
IL-23R Antibody
NB600-1147
Simple Western IL-23R Antibody
Western Blot IL-23R Antibody
Western Blot IL-23R Antibody
(1 Review)
(8 Publications)
Species: Hu, Mu
Applications: Flow-CS, Flow, IHC, IHC-P, Simple Western, WB
ATG16L1 Antibody -
BSA Free
NB110-60928
Western Blot ATG16L1 Antibody - BSA Free
Western Blot ATG16L1 Antibody - BSA Free
Immunohistochemistry-Paraffin ATG16L1 Antibody - BSA Free
(1 Review)
(11 Publications)
Species: Al, Bv, Ca, Hu, Mu, Pm, Rt
Applications: EM, IHC, IHC-P, WB
IL-12/IL-23 p40
[Biotin]
DY499
N/A IL-12/IL-23 p40 [Biotin]
(51 Publications)
Species: Mu
Applications: ELISA
IL-22
[Unconjugated]
782-IL/CF
SDS-PAGE IL-22 [Unconjugated]
Bioactivity IL-22 [Unconjugated]
(65 Publications)
Species: Hu
Applications: BA
STAT3 Antibody
(232209) [Unconjugate
MAB1799
Knockout Validated STAT3 Antibody (232209) [Unconjugated]
Western Blot STAT3 Antibody (232209) [Unconjugated]
Knockout Validated STAT3 Antibody (232209) [Unconjugated]
(23 Publications)
Species: Hu, Mu, Rt
Applications: ICC, IP, ICFlow, KO, WB
CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen CD4 Antibody - BSA Free
Immunohistochemistry-Paraffin CD4 Antibody - BSA Free
Flow Cytometry CD4 Antibody - BSA Free
(9 Reviews)
(50 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(665 Publications)
Species: Mu
Applications: ELISA
IL-17D Antibody
(246018) [Unconjugate
MAB15041
Immunohistochemistry IL-17D Antibody (246018) [Unconjugated]
Intracellular Staining by Flow Cytometry IL-17D Antibody (246018) [Unconjugated]
Species: Hu
Applications: CyTOF-ready, IHC, ICFlow, WB
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(302 Publications)
Species: Mu
Applications: ELISA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(818 Publications)
Species: Hu
Applications: BA
IL-21 Antibody
[Unconjugated]
AF594
Immunocytochemistry IL-21 Antibody [Unconjugated]
Neutralization IL-21 Antibody [Unconjugated]
(80 Publications)
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, Neut, WB
ROR
gamma/RORC/NR1F3 Anti ...
NBP2-24503
Immunohistochemistry-Paraffin ROR gamma/RORC/NR1F3 Antibody - BSA Free
Western Blot ROR gamma/RORC/NR1F3 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence ROR gamma/RORC/NR1F3 Antibody - BSA Free
(1 Review)
(3 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
TEAD1 Antibody -
Azide and BSA Free
NBP2-94035
Chromatin Immunoprecipitation TEAD1 Antibody - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence TEAD1 Antibody - Azide and BSA Free
Western Blot TEAD1 Antibody - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: ChIP, ELISA, ICC/IF, IHC, IHC-P, WB
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(455 Publications)
Species: Hu
Applications: BA
OCTN1/SLC22A4
Antibody
H00006583-A01
Western Blot OCTN1/SLC22A4 Antibody
(1 Review)
(3 Publications)
Species: Ca, Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC-WhMt, IHC, IHC-Fr, WB
ACAD8 Antibody
NBP2-47559
Western Blot ACAD8 Antibody
Immunohistochemistry-Paraffin ACAD8 Antibody
Immunohistochemistry-Paraffin ACAD8 Antibody
Species: Hu
Applications: IHC, IHC-P, WB

Publications for IL-23R Lysate (NBL1-11944) (0)

There are no publications for IL-23R Lysate (NBL1-11944).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IL-23R Lysate (NBL1-11944) (0)

There are no reviews for IL-23R Lysate (NBL1-11944). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-23R Lysate (NBL1-11944) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IL-23R Products

Research Areas for IL-23R Lysate (NBL1-11944)

Find related products by research area.

Blogs on IL-23R

There are no specific blogs for IL-23R, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005
Simple Western: IL-23 R Antibody [NB600-1147] - Lane view shows a specific band for IL23 Receptor in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
IL-23R Antibody
NB600-1147-0.2ml

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IL-23R Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol IL23R