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Recombinant Sheep IL-22 Protein

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ELISA: Recombinant Sheep IL-22 Protein [NBP3-11060] - Recombinant Sheep Interleukin-22 detected using Rabbit anti Sheep Interleukin-22 as the capture reagent and Rabbit anti Sheep Interleukin-22:Biotin as the detection reagent followed by Streptavidin:HRP.
ELISA: Recombinant Sheep IL-22 Protein [NBP3-11060] - Recombinant Sheep Interleukin-22 detected using Rabbit anti Sheep Interleukin-22 as the capture reagent and Rabbit anti Sheep Interleukin-22:Biotin as the detection ...read more

Product Details

Summary
Reactivity ShSpecies Glossary
Applications WB, ELISA
Concentration
LYOPH

Order Details

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Recombinant Sheep IL-22 Protein Summary

Description
Recombinant Sheep Interleukin-22 (IL-22) is a recombinant protein corresponding to aa 34-190 of native sheep IL-22.
Preparation
Method
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography.
Protein/Peptide Type
Recombinant Protein
Gene
IL22
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot
Theoretical MW
18.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
10% Trehalose
Concentration
LYOPH
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Reconstitute with 20 uL sterile PBS containing 0.1% carrier protein. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution.

Alternate Names for Recombinant Sheep IL-22 Protein

  • Cytokine Zcyto18
  • IL-10-related T-cell-derived inducible factor
  • IL22
  • IL-22
  • IL-D110
  • IL-TIF
  • ILTIFIL-10-related T-cell-derived-inducible factor
  • IL-TIFMGC79382
  • interleukin 22
  • interleukin-22
  • MGC79384
  • TIFa
  • TIFIL-23
  • zcyto18

Background

Interleukin-22 (IL-22 or IL-TIF (IL-10-related T-cell derived inducible factor)) belongs to the IL-10 family of regulatory cytokines which also includes IL-10, IL-19, IL-20, IL-24 and IL-26. IL-22 is produced by T lymphocytes following anti-CD3 stimulation. It inhibits IL-4 production of Th2 cells, activates STAT-1 and STAT-3 in several hepatoma cell lines and upregulates the production of acute phase proteins. It may also be involved in inflammatory responses. Human and mouse IL-22 share 79% amino acid identity. Recombinant human IL-22 is a non-disulfide linked homodimer, signaling through a receptor complex of two subunits, IL-22R and IL-10R-beta, belonging to the class II cytokine receptor family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for IL-22 Recombinant Protein (NBP3-11060) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol IL22
Uniprot