Home » IL-2 » IL-2 Antibodies » IL-2 Antibody [Unconjugated]

IL-2 Antibody [Unconjugated]

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Recombinant Porcine IL‑2 (Catalog # 652-P2) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑2 (2 ng/mL) is neutralized (green line) by increasing concen­trations of Goat Anti-Porcine IL‑2 Antigen Affinity-purified Poly­clonal Antibody (Catalog # AF652). The ND50 is typically0.25-0.8 µg/mL.
Recombinant Porcine IL‑2 (Catalog # 652-P2) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑2 (2 ng/mL) ...read more
IL-2 was detected in immersion fixed porcine peripheral blood mononuclear cells treated with calcium ionomycin and PMA using Goat Anti-Porcine IL-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF652) at 25 ...read more

Product Details

Summary
Reactivity PoSpecies Glossary
Applications WB, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF652
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Catalog# & Formulation Size Price
Carrier Free
AF652-SP
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IL-2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant porcine IL-2
Ala21-Thr154
Accession # P26891
Specificity
Detects porcine IL‑2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IL-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL2
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 0.25-0.8 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publication using
AF652 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-2 Antibody [Unconjugated]

  • Aldesleukin
  • IL2
  • IL-2
  • IL-2lymphokine
  • interleukin 2
  • interleukin-2
  • involved in regulation of T-cell clonal expansion
  • Proleukin
  • T cell growth factor
  • T-cell growth factor
  • TCGF

Background

Interleukin 2 was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens. Since then, it has been shown that in addition to its T cell growth factor activity, IL-2 can also stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages and oligodendrocytes. Mature porcine and human IL-2 share approximately 72% amino acid sequence identity.The biological activity of IL-2 is mediated by the binding of IL-2 to cell surface receptor complexes. The functional high-affinity receptor of IL-2 is composed of three distinct polypeptide chains, the IL-2 receptor alpha , beta and gamma  subunits. The intermediate-affinity IL-2 receptor complex, which lacks the alpha subunit, but contains both the beta and gamma  subunits, is also capable of transducing the IL-2 signal. In T cells, the beta and gamma subunits are shared with the IL-15 receptor complex. The gamma chain of the IL-2 receptor complex is also a subunit of IL-4, IL-7, and IL-9 receptor complexes.

  1. Taniguchi, T. and Y. Minami (1993) Cell 73:5.
  2. Waldmann, T. et al. (1998) Int. Rev. Immunol. 16:205.
  3. Nelson, B.H. and D.M. Willeford (1998) Adv. Immunol. 70:1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-2 Antibody (AF652)(1)

We have publications tested in 1 confirmed species: Porcine.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol IL2
Uniprot