Home » IL-18R alpha/IL-1 R5 » IL-18R alpha/IL-1 R5 Antibodies » IL-18R alpha/IL-1 R5 Antibody (70625) [Unconjugated]
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IL-18R alpha/IL-1 R5 Antibody (70625) [Unconjugated]

 (13 Publications)
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In the presence of Recombinant Human TNF‑ alpha (20 ng/mL, Catalog # 210-TA), Recombinant Human IL‑18/IL‑1F4 (Catalog # B003-5) stimulates IFN‑ gamma secretion in KG-1 human myeloid leukemia cells in a dose-dependent manner (orange line), as measured by the Human IFN‑ gamma Quantikine ELISA Kit (Catalog # DIF50). Under these conditions, IFN‑ gamma secretion elicited by Recombinant Human IL‑18/IL‑1F4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑18 R alpha /IL‑1 R5 Monoclonal Antibody (Catalog # MAB840). The ND50 is typically 0.03‑0.1 µg/mL.
In the presence of Recombinant Human TNF‑ alpha (20 ng/mL, Catalog # 210-TA), Recombinant Human IL‑18/IL‑1F4 (Catalog # B003-5) stimulates IFN‑ gamma secretion in KG-1 human myeloid leukemia cells in a ...read more
IL-18 Ra/IL-1 R5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Human IL-18 Ra/IL-1 R5 Mono-clonal Antibody (Catalog # MAB840) for 3 hours at room ...read more
Soluble interleukin-18 receptor alpha complex is associated with interleukin-18 and the soluble form of the interleukin-18 receptor beta chain. (A) Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of ...read more
IL‑18 R alpha /IL‑1 R5 was detected in immersion fixed paraffin-embedded sections of Human Skin using Mouse Anti-Human IL‑18 R alpha /IL‑1 R5 Monoclonal Antibody (Catalog ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, B/N, CyTOF-ready, ICC/IF
Clone
70625
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB840U-100UG
Alexa Fluor 405
FAB840V-100UG
Alexa Fluor 488
FAB840G-100
Alexa Fluor 488
FAB840G-025
Alexa Fluor 532
FAB840X
Alexa Fluor 594
FAB840T-100UG
Alexa Fluor 647
FAB840R-100UG
Alexa Fluor 700
FAB840N-100UG
Alexa Fluor 750
FAB840S-100UG
Allophycocyanin
FAB840A-025
Allophycocyanin
FAB840A-100
Allophycocyanin/Cy7
FAB840APCCY7
Biotin
FAB840B
CoraFluor 1
FAB840CL1
DyLight 350
FAB840D
DyLight 405
FAB840E
DyLight 488
FAB840K
DyLight 550
FAB840L
DyLight 594
FAB840M
DyLight 650
FAB840W
DyLight 680
FAB840Y
DyLight 755
FAB840Z
FITC
FAB840F
HRP
FAB840H
Janelia Fluor 525
FAB840JF525
Janelia Fluor 549
FAB840I
Janelia Fluor 585
FAB840JF585
Janelia Fluor 635
FAB840JF635
Janelia Fluor 646
FAB840J
Janelia Fluor 669
FAB840JF669
PE/Atto594
FAB840PEATT594
PE/Cy5.5
FAB840PECY55
PE/Cy7
FAB840PECY7
Phycoerythrin
FAB840P-025
Phycoerythrin
FAB840P-100
mFluor Violet 450 SE
FAB840MFV450
mFluor Violet 500 SE
FAB840MFV500
mFluor Violet 610 SE
FAB840MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB840A-100
Carrier Free
FAB840G-100
Carrier Free
FAB840P-100
Carrier Free
FAB840A-025
Carrier Free
FAB840G-025
Carrier Free
FAB840P-025
Carrier Free
MAB840-SP
Carrier Free
MAB840-500
Carrier Free
FAB840V-100UG
Carrier Free
FAB840U-100UG
Carrier Free
FAB840T-100UG
Carrier Free
FAB840S-100UG
Carrier Free
FAB840R-100UG
Carrier Free
FAB840N-100UG
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IL-18R alpha/IL-1 R5 Antibody (70625) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL-1 R5
Glu20-Arg329
Accession # Q13478
Specificity
Detects Recombinant Human IL-18 R alpha /IL-1 R5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-1 R3, rhIL-1 R4, rhIL-1 R6, rhIL-1 R7, rhIL-1 R8, rhIL-1 R9, rhSIGIRR or recombinant mouse IL-18 R alpha is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL18R1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Neutralization 0.03-0.1 ug/mL
Publications
Read Publications using
MAB840 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-18R alpha/IL-1 R5 Antibody (70625) [Unconjugated]

  • CD218a
  • CD218a;CDw218a;IL18R1;IL18RA;IL-18Ralpha;IL-18R-alpha;IL18Ralpha2;IL1RRP;IL-1Rrp;Interleukin-18 receptor 1
  • IL-1 R5
  • IL-1 Rrp
  • IL-18 R alpha
  • IL-18 R1
  • IL18R alpha
  • IL18R1
  • IL-18Ra

Background

Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL‑18 R beta is required for high affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.

  1. Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
  2. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
  3. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IL-18R alpha/IL-1
R5 Antibody (70625) [ ...
MAB840
IL-18R alpha/IL-1 R5 Antibody (70625) [Unconjugated]
Species: Hu
Applications: Flow, IHC, B/N, CyTOF-ready, ICC/IF

Publications for IL-18R alpha/IL-1 R5 Antibody (MAB840)(13)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 6 applications: Flow Cytometry, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.


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Flow Cytometry
(5)
IHC
(1)
IHC-Fr
(1)
IHC-P
(2)
Neutralization
(3)
Western Blot
(1)
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Human
(11)
Rat
(1)
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using MAB840 Applications Species
Jin-Min Sun, Hong-Ye Fan, Yan Zhu, Ting-Ting Pan, Yong-Ping Wu, Dao-Yong Zhang et al. Glioblastoma cellular MAP4K1 facilitates tumor growth and disrupts T effector cell infiltration Life Sci Alliance 9/21/2023 [PMID: 37734869]
Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma Sci Rep, 2016-05-26;6(0):26757. 2016-05-26 [PMID: 27225603] (IHC-P, Human) IHC-P Human
Wang J, Sun C, Gerdes N, Liu C, Liao M, Liu J, Shi M, He A, Zhou Y, Sukhova G, Chen H, Cheng X, Kuzuya M, Murohara T, Zhang J, Cheng X, Jiang M, Shull G, Rogers S, Yang C, Ke Q, Jelen S, Bindels R, Ellison D, Jarolim P, Libby P, Shi G Interleukin 18 function in atherosclerosis is mediated by the interleukin 18 receptor and the Na-Cl co-transporter. Nat Med, 2015-06-22;21(7):820-6. 2015-06-22 [PMID: 26099046] (IHC-Fr, Human) IHC-Fr Human
Pesce S, Carlomagno S, Moretta A, Sivori S, Marcenaro E Uptake of CCR7 by KIR2DS4(+) NK cells is induced upon recognition of certain HLA-C alleles. J Immunol Res, 2015-04-16;2015(0):754373. 2015-04-16 [PMID: 25961063] (Flow Cytometry, Human) Flow Cytometry Human
Horbelt D, Boergermann J, Chaikuad A, Alfano I, Williams E, Lukonin I, Timmel T, Bullock A, Knaus P Small molecules dorsomorphin and LDN-193189 inhibit myostatin/GDF8 signaling and promote functional myoblast differentiation. J Biol Chem, 2014-11-03;290(6):3390-404. 2014-11-03 [PMID: 25368322] (Neutralization, Human) Neutralization Human
Guo W, Miyoshi K, Dubner R, Gu M, Li M, Liu J, Yang J, Zou S, Ren K, Noguchi K, Wei F Spinal 5-HT3 receptors mediate descending facilitation and contribute to behavioral hypersensitivity via a reciprocal neuron-glial signaling cascade. Mol Pain, 2014-06-09;10(0):35. 2014-06-09 [PMID: 24913307] (IHC, Rat) IHC Rat
Li W, Okuda A, Yamamoto H, Yamanishi K, Terada N, Yamanishi H, Tanaka Y, Okamura H Regulation of development of CD56 bright CD11c + NK-like cells with helper function by IL-18. PLoS ONE, 2013-12-20;8(12):e82586. 2013-12-20 [PMID: 24376549] (Flow Cytometry, Human) Flow Cytometry Human
Blom L, Poulsen L IL-1 Family Members IL-18 and IL-33 Upregulate the Inflammatory Potential of Differentiated Human Th1 and Th2 Cultures. J Immunol, 2012-10-01;189(9):4331-7. 2012-10-01 [PMID: 23028054] (Neutralization, Human) Neutralization Human
Takei S, Hoshino T, Matsunaga K, Sakazaki Y, Sawada M, Oda H, Takenaka S, Imaoka H, Kinoshita T, Honda S, Ida H, Fukuda TA, Aizawa H Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis. Arthritis Res. Ther., 2011-03-24;13(2):R52. 2011-03-24 [PMID: 21435242] (Western Blot, Human) Western Blot Human
Tucci M, Quatraro C, Lombardi L, Pellegrino C, Dammacco F, Silvestris F Glomerular accumulation of plasmacytoid dendritic cells in active lupus nephritis: role of interleukin-18. Arthritis Rheum., 2008-01-01;58(1):251-62. 2008-01-01 [PMID: 18163476] (Flow Cytometry, IHC-P, Human) Flow Cytometry, IHC-P Human
Show All 13 Publications.

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Bioinformatics

Gene Symbol IL18R1
Uniprot