Reactivity | HuSpecies Glossary |
Applications | Flow |
Clone | 174113 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Alexa Fluor 594 |
Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
Immunogen | E. coli-derived recombinant human IL-17B Gln21-Phe180 Accession # Q9UHF5 |
Specificity | Detects human IL-17B in Western blots and ELISAs. In Western blots, approximately 25% cross-reactivity with recombinant mouse IL‑17B is observed and no cross-reactivity with recombinant human (rh) IL‑17, rhIL-17C, rhIL-17E or rhIL-17F is observed. In sandwich immunoassays, less than 0.5% cross-reactivity with rmIL-17B, and no cross-reactivity with rhIL-17C, rhIL-17D, rhIL-17E, rhIL-17F, or rhIL-17 is observed. |
Isotype | IgG2b |
Clonality | Monoclonal |
Host | Mouse |
Gene | IL17B |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells. |
Storage | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
Buffer | Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. |
Preservative | 0.09% Sodium Azide |
Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus (1, 2). With the exception of IL-17B, which exists as a non‑covalently linked dimer, all IL-17 family members are disulfide-linked dimers (3). IL-17 family proteins are pro‑inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Two receptors (IL‑17 R, and IL-17B R), which are activated by IL-17 family members, have been identified. In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL‑17 RE, have also been reported (1‑4). Human IL-17B cDNA encodes a 180 aa protein with a putative 20 aa signal peptide (5, 6). Human and mouse IL-17B share 88% amino acid sequence identity. Among IL-17 family members, IL-17B is most closely related to IL-17D, sharing 27% aa sequence homology. IL-17B is expressed highly in spinal cord, and at lower levels in brain, kidney, lung, small intestine, prostate, testes, pancreas, adrenal gland and trachea (5‑7). Expression of IL-17B has also been detected in chondrocytes in articular cartilage (2). IL-17B binds the IL-17B receptor but not IL-17 R and exhibits bioactivities distinct from those of IL-17 (5, 6).
Secondary Antibodies |
Isotype Controls |
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