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Recombinant Human IL-24 Protein

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IL-24 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human IL-20 R alpha and human IL-20 R beta . The ED50 for this effect is 15-150 pg/mL.
Source
Mouse myeloma cell line, NS0-derived human IL-24 protein
Met28-Leu206, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Gln52 predicted, no results obtained, sequencing might be blocked.
Protein/Peptide Type
Recombinant Proteins
Gene
IL24
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
19.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
26-35 kDa, reducing conditions
Publications
Read Publications using
1965-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-24 Protein

  • C49A
  • FISP
  • IL10B
  • IL24
  • IL-24
  • interleukin 24
  • MDA-7
  • MDA7MOB5
  • melanocyte-associated Mda-7
  • melanoma differentiation association protein 7
  • Melanoma differentiation-associated gene 7 protein
  • MOB-5
  • ST16
  • ST16interleukin-24
  • suppression of tumorigenicity 16 (melanoma differentiation)
  • Suppression of tumorigenicity 16 protein

Background

Interleukin 24 (IL-24), also known as mda-7 (melanoma differentiation associated gene-7), is a member of the IL-10 family of helical cytokines. The IL-24 gene encodes a precursor protein of 207 amino acids (aa) that contains a 48 aa signal sequence and an 18 kDa, 158 aa mature segment. There are three potential N-linked glycosylation sites, at least one of which is used. When secreted, IL-24 is a 35 - 40 kDa phosphorylated glycoprotein that apparently can exist as either a monomer or dimer. It is suggested that glycosylation is essential for activity. Mature human IL-24 shares 69% aa sequence identity with mouse and rat IL-24. Human IL-24 is also active in rodent systems. Cells known to express IL-24 include B cells, CD4+ T cells, NK cells, lymph node dendritic cells, monocytes, melanocytes, and melanoma cells. Functionally, IL‑24 has diverse activities. At low concentrations on monocytes, it induces type I proinflammatory cytokines such as IFN‑ gamma , IL‑1 beta , IL‑12 and TNF-alpha . At high concentrations, it is a strong inducer of apoptosis in tumor cells, but not normal cells. IL‑24 also has anti-angiogenic properties. It directly binds IL-24 receptors on endothelial cells, activating STAT3 and blocking their differentiation. IL-24 binds and signals through two heterodimeric receptor complexes. One complex is the combination of IL‑20 R alpha and IL-20 R beta , which is shared with IL-19 and IL‑20. The second complex is a combination of IL‑22 R and IL-20 R beta , which is shared with IL-20.

  1. Jiang, H. et al. (1995) Oncogene 11:2477.
  2. Jiang, H. et al. (1996) Proc. Natl. Acad. Sci. USA 93:9160.
  3. Wang, M. et al. (2002) J. Biol Chem. 227:7341.
  4. Chada, S. et al. (2004) Int. Immunopharmacol. 4:649.
  5. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  6. Chen, J. et al. (2003) Molec. Ther. 8:220.

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1965-IL
Species: Hu
Applications: Bioactivity

Publications for IL-24 (1965-IL)(19)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: Bioassay, ELISA (Standard).


Filter By Application
Bioassay
(18)
ELISA (Standard)
(1)
All Applications
Filter By Species
Human
(18)
Rat
(1)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using 1965-IL Applications Species
Seong, SH;Kim, JY;Kim, SH;Lee, J;Lee, EJ;Bae, YJ;Park, S;Kwon, IJ;Yoon, SM;Lee, J;Kim, TG;Oh, SH; Interleukin-24: A molecular mediator of particulate matter's impact on skin aging Ecotoxicology and environmental safety 2024-07-18 [PMID: 39029221] (Bioassay, Human) Bioassay Human
Francis, L;McCluskey, D;Ganier, C;Jiang, T;Du-Harpur, X;Gabriel, J;Dhami, P;Kamra, Y;Visvanathan, S;Barker, JN;Smith, CH;Capon, F;Mahil, SK; Single-cell analysis of psoriasis resolution demonstrates an inflammatory fibroblast state targeted by IL-23 blockade Nature communications 2024-01-30 [PMID: 38291032] (Bioassay, Human) Bioassay Human
Y Liu, Y Chen, R Sha, Y Li, T Xu, X Hu, L Xu, Q Xie, B Zhao A new insight into the role of aryl hydrocarbon receptor (AhR) in the migration of glioblastoma by AhR-IL24 axis regulation Environment international, 2021-05-31;154(0):106658. 2021-05-31 [PMID: 34082239] (Bioassay, Human) Bioassay Human
D Pap, A Veres-Szék, B Szebeni, R Rokonay, A Ónody, R Lippai, IM Takács, A Tislér, M Kardos, F Oswald, A Fekete, AJ Szabó, Á Vannay Characterization of IL-19, -20, and -24 in acute and chronic kidney diseases reveals a pro-fibrotic role of IL-24 J Transl Med, 2020-04-19;18(1):172. 2020-04-19 [PMID: 32306980] (Bioassay, Human, Rat) Bioassay Human, Rat
D Deli?, K Wolk, R Schmid, O Gabrielyan, D Christou, K Rieber, M Rolser, I Jakob, F Wiech, M Griesser, C Wohnhaas, G Kokolakis, E Witte-Händ, P Baum, R Sabat Integrated microRNA/mRNA expression profiling of the skin of psoriasis patients J. Dermatol. Sci., 2019-11-30;0(0):. 2019-11-30 [PMID: 31843230] (Bioassay, Human) Bioassay Human
NP Kumar, K Moideen, VV Banurekha, D Nair, S Babu Modulation of Th1/Tc1 and Th17/Tc17 responses in pulmonary tuberculosis by IL-20 subfamily of cytokines Cytokine, 2018-04-21;108(0):190-196. 2018-04-21 [PMID: 29684756] (Bioassay, Human) Bioassay Human
S Kawada, Y Nagasawa, M Kawabe, H Ohyama, A Kida, N Kato-Kogoe, M Nanami, Y Hasuike, T Kuragano, H Kishimoto, K Nakasho, T Nakanishi Iron-induced calcification in human aortic vascular smooth muscle cells through interleukin-24 (IL-24), with/without TNF-alpha Sci Rep, 2018-01-12;8(1):658. 2018-01-12 [PMID: 29330517] (Bioassay, Human) Bioassay Human
BX Ross, N Gao, X Cui, TJ Standiford, J Xu, FX Yu IL-24 Promotes Pseudomonas aeruginosa Keratitis in C57BL/6 Mouse Corneas J. Immunol, 2017-03-22;0(0):. 2017-03-22 [PMID: 28330899] (Bioassay, Human) Bioassay Human
D Pap, E Sziksz, Z Kiss, R Rokonay, A Veres-Szék, R Lippai, IM Takács, É Kis, A Fekete, G Reusz, A Vannay, AJ Szabó Microarray Analysis Reveals Increased Expression of Matrix Metalloproteases and Cytokines of Interleukin-20 Subfamily in the Kidneys of Neonate Rats Underwent Unilateral Ureteral Obstruction: A Potential Role of IL-24 in the Regulation of Inflammation and Tissue Remodeling Kidney Blood Press. Res, 2017-02-28;42(1):16-32. 2017-02-28 [PMID: 28253513] (Bioassay, Human) Bioassay Human
Robert Sabat MMP8 Is Increased in Lesions and Blood of Acne Inversa Patients: A Potential Link to Skin Destruction and Metabolic Alterations Mediators Inflamm., 2016-10-23;2016(0):4097574. 2016-10-23 [PMID: 27843200] (Bioassay, Human) Bioassay Human
Show All 19 Publications.

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Bioinformatics

Gene Symbol IL24
Uniprot