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IL-10R alpha Antibody (OTI1D10) - Azide and BSA Free

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Western Blot: IL-10R alpha Antibody (OTI1D10) - Azide and BSA Free [NBP2-71030] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-10 R alpha.
Western Blot: IL-10R alpha Antibody (OTI1D10) - Azide and BSA Free [NBP2-71030] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-10 R alpha.
Immunohistochemistry: IL-10R alpha Antibody (OTI1D10) - Azide and BSA Free [NBP2-71030] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: IL-10R alpha Antibody (OTI1D10) - Azide and BSA Free [NBP2-71030] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: IL-10R alpha Antibody (OTI1D10) - Azide and BSA Free [NBP2-71030] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
OTI1D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71030AF350
Alexa Fluor 405
NBP2-71030AF405
Alexa Fluor 488
NBP2-71030AF488
Alexa Fluor 532
NBP2-71030AF532
Alexa Fluor 594
NBP2-71030AF594
Alexa Fluor 647
NBP2-71030AF647
Alexa Fluor 700
NBP2-71030AF700
Alexa Fluor 750
NBP2-71030AF750
Allophycocyanin
NBP2-71030APC
Biotin
NBP2-71030B
CoraFluor 1
NBP2-71030CL1
DyLight 350
NBP2-71030UV
DyLight 405
NBP2-71030V
DyLight 488
NBP2-71030G
DyLight 550
NBP2-71030R
DyLight 594
NBP2-71030DL594
DyLight 650
NBP2-71030C
DyLight 680
NBP2-71030FR
DyLight 755
NBP2-71030IR
FITC
NBP2-71030F
HRP
NBP2-71030H
Janelia Fluor 525
NBP2-71030JF525
Janelia Fluor 549
NBP2-71030JF549
Janelia Fluor 646
NBP2-71030JF646
Janelia Fluor 669
NBP2-71030JF669
PE
NBP2-71030PE
PerCP
NBP2-71030PCP
Unconjugated
NBP2-46049
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46049
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IL-10R alpha Antibody (OTI1D10) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human IL10RA(NP_001549) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL10RA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:4000
Theoretical MW
60.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for IL-10R alpha Antibody (OTI1D10) - Azide and BSA Free

  • CD210 antigen
  • CDw210a
  • HIL-10R
  • IBD28
  • IL-10 R alpha
  • IL-10 receptor subunit alpha
  • IL10R alpha
  • IL-10R subunit 1
  • IL-10R1
  • IL10RA
  • IL-10Ra
  • IL10RIL-10R subunit alpha
  • interleukin 10 receptor, alpha
  • interleukin-10 receptor alpha chain
  • Interleukin-10 receptor subunit 1
  • interleukin-10 receptor subunit alpha

Background

The IL-10 receptor, IL-10R, is a member of the class II subgroup of the cytokine receptor family and exhibits structural similarity to the interferon receptor. IL-10R is expressed in B cells and T helper cells, as well as in LPS-induced mouse fibroblasts. Overall, mouse IL-10R and human IL-10R share 60% sequence identity at the protein level. Stimulation with IL-10 leads to tyrosine phosphorylation of JAK1 and Tyk2, but not JAK2 or JAK3. In addition to its role as an antagonist of IFN-gamma-mediated macrophage activation, IL-10Ralpha transmits differentiation as well as proliferative signals. IL-10R is constitutively expressed on human natural killer (NK) cells and the direct binding of IL-10 potentiates cytokine production by human NK cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IL10RA