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IL-10 Antibody (25209) [Unconjugated]

 (17 Publications)
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Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB2171) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human IL-10 Monoclonal ...read more
Recombinant Human IL‑10 (Catalog # 217-IL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑10 (5 ng/mL) is ...read more
S100A8 and S100A9 mRNA expression increases were partially abrogated by IL-10 blockade.Messenger RNA (mRNA) level of TNF alpha , IL-10, S100A8 and S100A9 in an ex vivo model of endotoxin tolerance in the presence or ...read more
S100A8 and S100A9 mRNA expression increases were partially abrogated by IL-10 blockade.Messenger RNA (mRNA) level of TNF alpha , IL-10, S100A8 and S100A9 in an ex vivo model of endotoxin tolerance in the presence or ...read more
S100A8 and S100A9 mRNA expression increases were partially abrogated by IL-10 blockade.Messenger RNA (mRNA) level of TNF alpha , IL-10, S100A8 and S100A9 in an ex vivo model of endotoxin tolerance in the presence or ...read more
S100A8 and S100A9 mRNA expression increases were partially abrogated by IL-10 blockade.Messenger RNA (mRNA) level of TNF alpha , IL-10, S100A8 and S100A9 in an ex vivo model of endotoxin tolerance in the presence or ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clone
25209
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB2171-SP
Carrier Free
MAB2171-500
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IL-10 Antibody (25209) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IL-10
Ser19-Asn178
Accession # P22301
Specificity
Detects human IL-10 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse IL‑10.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
IL10
Purity Statement
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Neutralization 0.005-0.03 ug/mL
  • Western Blot 5 ug/mL
Publications
Read Publications using
MAB2171 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-10 Antibody (25209) [Unconjugated]

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • GVHDS
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF

Background

IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-10 Antibody (MAB2171)(17)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Bioassay, Flow Cytometry, Neutralization.


Filter By Application
Bioassay
(1)
Flow Cytometry
(1)
Neutralization
(11)
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Filter By Species
Human
(12)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using MAB2171 Applications Species
von Glehn, F;Pochet, N;Thapa, B;Raheja, R;Mazzola, MA;Jangi, S;Beynon, V;Huang, J;Farias, AS;Paul, A;Santos, LMB;Gandhi, R;Murugaiyan, G;Weiner, HL;Baecher-Allan, CM; Defective Induction of IL-27-Mediated Immunoregulation by Myeloid DCs in Multiple Sclerosis International journal of molecular sciences 2023-04-28 [PMID: 37175706] (Neutralization, Human) Neutralization Human
MR Shokri, M Bozorgmehr, A Ghanavatin, R Falak, M Aleahmad, S Kazemnejad, F Shokri, AH Zarnani Human menstrual blood-derived stromal/stem cells modulate functional features of natural killer cells Sci Rep, 2019-07-10;9(1):10007. 2019-07-10 [PMID: 31292483] (Bioassay, Human) Bioassay Human
Hongxiao Song, Guangyun Tan, Yang Yang, An Cui, Haijun Li, Tianyang Li et al. Hepatitis B Virus–Induced Imbalance of Inflammatory and Antiviral Signaling by Differential Phosphorylation of STAT1 in Human Monocytes The Journal of Immunology 2019-04-15 [PMID: 30842274]
R Tzach-Nahm, R Nashef, O Fleissig, A Palmon, L Shapira, A Wilensky, G Nussbaum Oral fibroblasts modulate the macrophage response to bacterial challenge Sci Rep, 2017-09-14;7(1):11516. 2017-09-14 [PMID: 28912533] (Neutralization, Human) Neutralization Human
S Nakachi, S Sumitomo, Y Tsuchida, H Tsuchiya, M Kono, R Kato, K Sakurai, N Hanata, Y Nagafuchi, S Tateishi, H Kanda, T Okamura, K Yamamoto, K Fujio Interleukin-10-producing LAG3(+) regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis Arthritis Res. Ther., 2017-05-16;19(1):97. 2017-05-16 [PMID: 28511719] (Neutralization, Human) Neutralization Human
Fontaine M, Planel S, Peronnet E, Turrel-Davin F, Piriou V, Pachot A, Monneret G, Lepape A, Venet F S100A8/A9 mRNA induction in an ex vivo model of endotoxin tolerance: roles of IL-10 and IFNgamma. PLoS ONE, 2014-06-23;9(6):e100909. 2014-06-23 [PMID: 24956170] (Neutralization, Human) Neutralization Human
Planes R, BenMohamed L, Leghmari K, Delobel P, Izopet J, Bahraoui E HIV-1 Tat protein induces PD-L1 (B7-H1) expression on dendritic cells through tumor necrosis factor alpha- and toll-like receptor 4-mediated mechanisms. J Virol, 2014-04-02;88(12):6672-89. 2014-04-02 [PMID: 24696476] (Neutralization, Human) Neutralization Human
Svajger U, Obermajer N, Jeras M IFN-gamma-rich environment programs dendritic cells toward silencing of cytotoxic immune responses. J Leukoc Biol, 2013-08-07;95(1):33-46. 2013-08-07 [PMID: 23924658] (Neutralization, Human) Neutralization Human
Wang YC, Sung WW, Wu TC, Wang L, Chien WP, Cheng YW, Chen CY, Shieh SH, Lee H Interleukin-10 haplotype may predict survival and relapse in resected non-small cell lung cancer. PLoS ONE, 2012-07-27;7(7):e39525. 7/27/2012 [PMID: 22848356]
Nicolaidou V, Wong MM, Redpath AN Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation. PLoS ONE, 2012-07-03;7(7):e39871. 2012-07-03 [PMID: 22802946] (Neutralization, Human) Neutralization Human
Show All 17 Publications.

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Bioinformatics

Gene Symbol IL10
Uniprot