![Western Blot: IFITM3/Fragilis Antibody (JU73-02) [NBP2-75533] - Western blot analysis of IFITM3/Fragilis on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate](http://images.novusbio.com/fullsize/IFITM3-Fragilis-Antibody-JU73-02-Western-Blot-NBP2-75533-img0008.jpg "Western Blot: IFITM3/Fragilis Antibody (JU73-02) [NBP2-75533] - Western blot analysis of IFITM3/Fragilis on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | JU73-02 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human IFITM3/Fragilis aa 1-50 / 133. (SwissProt: Q01628 Human) |
| Localization | Cell membrane, Late endosome membrane, Lysosome membrane, perinuclear region. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | IFITM3 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 15 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | IFITM3 |
![Immunocytochemistry/Immunofluorescence: IFITM3/Fragilis Antibody (JU73-02) [NBP2-75533] - Staining Fragilis in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IFITM3-Fragilis-Antibody-JU73-02-Immunocytochemistry-Immunofluorescence-NBP2-75533-img0004.jpg "Immunocytochemistry/Immunofluorescence: IFITM3/Fragilis Antibody (JU73-02) [NBP2-75533] - Staining Fragilis in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: IFITM3/Fragilis Antibody (JU73-02) [NBP2-75533] - Analysis of paraffin-embedded human colon cancer tissue using anti-Fragilis antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IFITM3-Fragilis-Antibody-JU73-02-Immunohistochemistry-Paraffin-NBP2-75533-img0006.jpg "Immunohistochemistry-Paraffin: IFITM3/Fragilis Antibody (JU73-02) [NBP2-75533] - Analysis of paraffin-embedded human colon cancer tissue using anti-Fragilis antibody. Counter stained with hematoxylin.")
![Flow Cytometry: IFITM3/Fragilis Antibody (JU73-02) [NBP2-75533] - Analysis of MCF-7 cells with Fragilis antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/IFITM3-Fragilis-Antibody-JU73-02-Flow-Cytometry-NBP2-75533-img0001.jpg "Flow Cytometry: IFITM3/Fragilis Antibody (JU73-02) [NBP2-75533] - Analysis of MCF-7 cells with Fragilis antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.")
![Immunocytochemistry/Immunofluorescence: IFITM3/Fragilis Antibody (JU73-02) [NBP2-75533] - Staining Fragilis in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IFITM3-Fragilis-Antibody-JU73-02-Immunocytochemistry-Immunofluorescence-NBP2-75533-img0002.jpg "Immunocytochemistry/Immunofluorescence: IFITM3/Fragilis Antibody (JU73-02) [NBP2-75533] - Staining Fragilis in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: IFITM3/Fragilis Antibody (JU73-02) [NBP2-75533] - Staining Fragilis in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IFITM3-Fragilis-Antibody-JU73-02-Immunocytochemistry-Immunofluorescence-NBP2-75533-img0003.jpg "Immunocytochemistry/Immunofluorescence: IFITM3/Fragilis Antibody (JU73-02) [NBP2-75533] - Staining Fragilis in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: IFITM3/Fragilis Antibody (JU73-02) [NBP2-75533] - Analysis of paraffin-embedded human liver cancer tissue using anti-Fragilis antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IFITM3-Fragilis-Antibody-JU73-02-Immunohistochemistry-Paraffin-NBP2-75533-img0005.jpg "Immunohistochemistry-Paraffin: IFITM3/Fragilis Antibody (JU73-02) [NBP2-75533] - Analysis of paraffin-embedded human liver cancer tissue using anti-Fragilis antibody. Counter stained with hematoxylin.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")