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IFITM1 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining in human epididymis and testis tissues using anti-IFITM1 antibody. Corresponding IFITM1 RNA-seq data are presented for ...read more
Western Blot: IFITM1 Antibody [NBP1-89345] - Analysis in human cell line CAPAN-2.
Immunocytochemistry/ Immunofluorescence: IFITM1 Antibody [NBP1-89345] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining of human tonsil shows strong membranous positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining of human epididymis shows strong membranous positivity in ependymal cells.
Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining of human skeletal muscle shows no membranous positivity in myocytes as expected.
Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining of human testis shows no membranous positivity in cells in seminiferous ducts as expected.
Simple Western: IFITM1 Antibody [NBP1-89345] - Simple Western lane view shows a specific band for IFITM1 in 0.2 mg/ml of h. Tonsil lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Simple Western: IFITM1 Antibody [NBP1-89345] - Electropherogram image(s) of corresponding Simple Western lane view. IFITM1 antibody was used at 1:20 dilution on h. Tonsil lysate(s).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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IFITM1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MHKEEHEVAVLGAPPSTILPRSTVINIHSETSVPDH
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IFITM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Simple Western 1:20
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control Peptide
IFITM1 Protein (NBP1-89345PEP)
Publications
Read Publications using NBP1-89345.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24072182)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for IFITM1 Antibody

  • CD225 antigen
  • CD225
  • CD225Leu-13 antigen
  • IFI17
  • IFI17LEU13,9-27
  • IFITM1
  • interferon induced transmembrane protein 1 (9-27)
  • Interferon-induced protein 17
  • interferon-induced transmembrane protein 1
  • Interferon-inducible protein 9-27
  • Leu-13

Background

Implicated in the control of cell growth. Component of a multimeric complex involved in the transduction of antiproliferative and homotypic adhesion signals

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IFITM1 Antibody (NBP1-89345)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IFITM1 Antibody (NBP1-89345) (0)

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Secondary Antibodies

 

Isotype Controls

Additional IFITM1 Products

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Blogs on IFITM1

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Bioinformatics

Gene Symbol IFITM1