![Western Blot: IDO2 Antibody (1HC) [NBP2-21641] - Western Blot of Mouse Anti-IDO2 antibody. Lane 1: HEK293 no transgene cell extracts. Lane 2: mouse IDO-2 transgene expressed in 293HEK.Lane 3: mouse IDO-1 transgene expressed in 293HEK. IDO2 monoclonal Antibody. Secondary antibody: Dye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blocking Buffer overnight at 4C.Predicted/Observed size: 44.4 kDa, ~60 kDa for IDO 2. Other band: IDO2 splice variants and isoforms.](http://images.novusbio.com/fullsize/IDO2-Antibody-1HC-Western-Blot-NBP2-21641-img0002.jpg "Western Blot: IDO2 Antibody (1HC) [NBP2-21641] - Western Blot of Mouse Anti-IDO2 antibody. Lane 1: HEK293 no transgene cell extracts. Lane 2: mouse IDO-2 transgene expressed in 293HEK.Lane 3: mouse IDO-1 transgene expressed in 293HEK. IDO2 monoclonal Antibody. Secondary antibody: Dye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blocking Buffer overnight at 4C.Predicted/Observed size: 44.4 kDa, ~60 kDa for IDO 2. Other band: IDO2 splice variants and isoforms.")
| Reactivity | MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 1HC |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | This antibody was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | IDO2 Antibody (1HC) was produced in mouse by repeated immunizations with complete mouse IDO2 protein followed by hybridoma development. (Uniprot: Q8R0V5) |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | IDO2 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This product has been tested in western blot and is suitable for use in ELISA and IF. Specific conditions for reactivity should be optimized by the end user. |
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| Publications |
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| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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