ICAM-3/CD50 Antibody (Cal 3.34) [Unconjugated] Summary
Immunogen |
Recombinant human ICAM‑3/CD50 Fc Chimera |
Specificity |
Detects human ICAM‑3/CD50 in Western blots. ICAM‑3/CD50 was found not to recognize CHO cells expressing the human ICAM-1 protein. ICAM‑3/CD50 was found to recognize CHO cells expressing the human ICAM-3 protein. The epitope for ICAM‑3/CD50 is located on domain 4 of the human ICAM-3 molecule. |
Source |
N/A |
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
ICAM3 |
Purity Statement |
Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- CyTOF-ready
- Flow Cytometry 0.25 ug/10^6 cells
- Western Blot 1 ug/mL
|
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Preservative |
No Preservative |
Concentration |
LYOPH |
Reconstitution Instructions |
Sterile PBS to a final concentration of 0.5 mg/mL. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for ICAM-3/CD50 Antibody (Cal 3.34) [Unconjugated]
Background
Intercellular Adhesion Molecule-3 (ICAM-3, CD50), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alpha d/ beta 2.
ICAM‑3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM-3 has not been identified in the mouse.
- Hayflick, J. et al. (1998) Immunologic Res. 17:313.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Secondary Antibodies
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