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ICAM-1/CD54 Antibody (1H4) [PE]

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Flow Cytometry: ICAM-1/CD54 Antibody (1H4) [PE] [NB500-582] - Surface staining of human peripheral blood with anti-CD54 (1H4) PerCP.
Flow Cytometry: ICAM-1/CD54 Antibody (1H4) [PE] [NB500-582] - Surface staining of human peripheral blood with anti-CD54 (1H4) PerCP.
Flow Cytometry: ICAM-1/CD54 Antibody (1H4) [PE] [NB500-582] - Surface staining of U937 human histiocytic lymphoma cell line with anti-human CD54 (1H4) PE. Total viable cells were used for analysis.
Flow Cytometry: ICAM-1/CD54 Antibody (1H4) [PE] [NB500-582] - Expression of CD54 in normal human peripheral blood, as detected with anti-CD54 (1H4) biotin.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC
Clone
1H4
Clonality
Monoclonal
Host
Mouse
Conjugate
PE

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB110-58732AF350
Alexa Fluor 405
NB110-58732AF405
Alexa Fluor 488
NB110-58732AF488
Alexa Fluor 532
NB110-58732AF532
Alexa Fluor 594
NB110-58732AF594
Alexa Fluor 647
NB110-58732AF647
Alexa Fluor 700
NB110-58732AF700
Alexa Fluor 750
NB110-58732AF750
Allophycocyanin
NB110-81732
Allophycocyanin/Cy7
NB110-58732APCCY7
Biotin
NB500-584
CoraFluor 1
NB110-58732CL1
DyLight 350
NB110-58732UV
DyLight 405
NB110-58732V
DyLight 488
NB110-58732G
DyLight 550
NB110-58732R
DyLight 594
NB110-58732DL594
DyLight 650
NB110-58732C
DyLight 680
NB110-58732FR
DyLight 755
NB110-58732IR
FITC
NB500-583-100Tests
HRP
NB110-58732H
Janelia Fluor 525
NB110-58732JF525
Janelia Fluor 549
NB110-58732JF549
Janelia Fluor 646
NB110-58732JF646
Janelia Fluor 669
NB110-58732JF669
PE
NB110-58732PE
PE/Atto594
NB110-58732PEATT594
PE/Cy5.5
NB110-58732PECY55
PE/Cy7
NB110-58732PECY7
PerCP
NB110-58732PCP
Unconjugated
NB110-58732
mFluor Violet 450 SE
NB110-58732MFV450
mFluor Violet 500 SE
NB110-58732MFV500
mFluor Violet 610 SE
NB110-58732MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB110-58732
Azide and BSA Free
NB110-58732
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ICAM-1/CD54 Antibody (1H4) [PE] Summary

Immunogen
Raji cells and spleen cells fused with NS1 cells.
Specificity
This antibody (clone 1H4) reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ICAM1
Purity
Size Exclusion Chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS and 0.2% (w/v) high grade BSA (protease free)
Preservative
0.9% Sodium Azide
Purity
Size Exclusion Chromatography

Alternate Names for ICAM-1/CD54 Antibody (1H4) [PE]

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICAM-1/CD54
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NB500-582
ICAM-1/CD54 Antibody (1H4) [PE]
Species: Hu
Applications: WB, Flow, IHC

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Bioinformatics

Gene Symbol ICAM1