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ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free

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Flow (Cell Surface): ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Surface staining of human peripheral blood with anti-CD54 (1H4) FITC. Image using the FITC format of this antibody.
Flow (Cell Surface): ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Surface staining of human peripheral blood with anti-CD54 (1H4) FITC. Image using the FITC format of this antibody.
Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Expression of CD54 in normal human peripheral blood, as detected with anti-CD54 (1H4) biotin.
Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Fig. 1. Surface staining of U937 human histiocytic lymphoma cell line with anti-human CD54 (1H4) PE. Total viable cells were used for analysis. ...read more
Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Staining of human peripheral blood with anti-CD54 (1H4) APC.
Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Staining of human peripheral blood with anti-CD54 (1H4) Alexa Fluor (R) 700.
Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Surface staining of human peripheral blood with anti-CD54 (1H4) PerCP. Image from the PE version of this antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, ICC/IF
Clone
1H4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB110-58732AF350
Alexa Fluor 405
NB110-58732AF405
Alexa Fluor 488
NB110-58732AF488
Alexa Fluor 532
NB110-58732AF532
Alexa Fluor 594
NB110-58732AF594
Alexa Fluor 647
NB110-58732AF647
Alexa Fluor 700
NB110-58732AF700
Alexa Fluor 750
NB110-58732AF750
Allophycocyanin
NB110-81732
Allophycocyanin/Cy7
NB110-58732APCCY7
Biotin
NB500-584
CoraFluor 1
NB110-58732CL1
DyLight 350
NB110-58732UV
DyLight 405
NB110-58732V
DyLight 488
NB110-58732G
DyLight 550
NB110-58732R
DyLight 594
NB110-58732DL594
DyLight 650
NB110-58732C
DyLight 680
NB110-58732FR
DyLight 755
NB110-58732IR
FITC
NB500-583-100Tests
HRP
NB110-58732H
Janelia Fluor 525
NB110-58732JF525
Janelia Fluor 549
NB110-58732JF549
Janelia Fluor 646
NB110-58732JF646
Janelia Fluor 669
NB110-58732JF669
PE
NB110-58732PE
PE
NB500-582-100Tests
PE/Atto594
NB110-58732PEATT594
PE/Cy5.5
NB110-58732PECY55
PE/Cy7
NB110-58732PECY7
PerCP
NB110-58732PCP
mFluor Violet 450 SE
NB110-58732MFV450
mFluor Violet 500 SE
NB110-58732MFV500
mFluor Violet 610 SE
NB110-58732MFV610
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ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free Summary

Immunogen
Raji cells and spleen cells fused with NS1 cells.
Specificity
This antibody (clone 1H4) reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ICAM1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow (Cell Surface)
  • Flow Cytometry 1-4 ug/ml
  • Immunocytochemistry
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 5-10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry-Paraffin sections recommended dilution: 10 ug/ml Application Note: Prolonged fixation in buffered formalin can destroy the epitope. Hight temperature antigen unmasking technique is required.
Western blotting: Recommended dilution: 1-2 ug/ml; non-reducing conditions.

This antibody is CyTOF ready.
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
ICAM-1/CD54 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
(PBS), approx. pH 7.4; 0.2 um filter sterilized.
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Antibody (1H4) - Azid ...
NB110-58732
ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, ICC/IF

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Control Lysate(s)

ICAM-1/CD54 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ICAM1