Home » Axl » Axl ELISA Kits » Human Phospho-Axl DuoSet IC ELISA, 2 Plate
Novus Biologicals products are now on bio-techne.com

Human Phospho-Axl DuoSet IC ELISA, 2 Plate

 (3 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Human glioblastoma cell line, A172, was treated with conditioned media from the human lung cell line, WI38, for fifteen minutes to induce tyrosine phosphorylation of Axl. Serial dilutions of lysates were analyzed by (A) IP-Western blot and (B) this ELISA . IPs were done using an anti-Axl monoclonal antibody and anti-mouse IgG agarose. Immunoblots were incubated with a biotinylated anti-phosphotyrosine monoclonal antibody (Catalog # BAM1676) to detect phospho-Axl (p-Axl). Bands were visualized with Streptavidin-HRP (Cat # DY998) followed by chemiluminescent detection.
Human glioblastoma cell line, A172, was treated with conditioned media from the human lung cell line, WI38, for fifteen minutes to induce tyrosine phosphorylation of Axl. Serial dilutions of lysates were analyzed by ...read more
A172 cells were untreated or treated with conditioned media from the human lung cell line, WI38, for fifteen minutes. ELISA and IP-Western blot (inset) analyses were done using 100 μg and 400 μg of lysate, ...read more
A172 cells were treated with conditioned media from the human lung cell line WI38 for fifteen minutes. The indicated amounts of recombinant extracellular domains of human Axl (Catalog # 154-AL), human Dtk (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
Biotin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
DYC2228-5
Biotin
DYC2228E
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Human Phospho-Axl DuoSet IC ELISA, 2 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
AXL

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DYC2228-2.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Phospho-Axl DuoSet IC ELISA, 2 Plate

  • AI323647
  • ARK
  • AXL oncogene
  • AXL receptor tyrosine kinase
  • AXL transforming sequence/gene
  • Axl
  • EC 2.7.10
  • EC 2.7.10.1
  • JTK11
  • Tyro7
  • tyrosine-protein kinase receptor UFO
  • UFO
  • UFOoncogene AXL

Background

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse orthologs of chicken c-Eyk) constitute the TAM receptor tyrosine kinase subfamily. This RTK subfamily is characterized by an extracellular domain that consists of two Ig-like motifs and two fibronectin type III motifs. These receptors bind the vitamin K-dependent protein Growth Arrest Specific Gene 6 (Gas6). Receptor activation leads to cell proliferation, migration, or the prevention of apoptosis. Cellular signaling through this family of RTKs is involved in hematopoiesis, embryonic development, tumorigenesis, and spermatogenesis.

Customers Who Viewed This Item Also Viewed...

Gas6 [Unconjugated]
885-GSB
Bioactivity Gas6 [Unconjugated]
(1 Review)
(38 Publications)
Species: Hu
Applications: BA
Dtk/TYRO3 Antibody
[Unconjugated]
AF759
Immunohistochemistry Dtk/TYRO3 Antibody [Unconjugated]
(13 Publications)
Species: Mu
Applications: IHC, WB
Mer Antibody
[Unconjugated]
AF591
Flow Cytometry Mer Antibody [Unconjugated]
Immunohistochemistry Mer Antibody [Unconjugated]
Dual RNAscope ISH-IHC Compatible Mer Antibody [Unconjugated]
(2 Reviews)
(71 Publications)
Species: Mu
Applications: CyTOF-ready, Dual ISH-IHC, Flow, IHC
GPER/GPR30
Antibody [Unconjugate
AF5534
Western Blot GPER/GPR30 Antibody [Unconjugated]
Immunohistochemistry GPER/GPR30 Antibody [Unconjugated]
Flow Cytometry GPER/GPR30 Antibody [Unconjugated]
(9 Publications)
Species: Hu
Applications: Flow, IHC, WB
AKT [p Ser473]
Antibody [Unconjugate ...
AF887
Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
(50 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
EGFR Antibody
[Unconjugated]
AF231
Immunohistochemistry EGFR Antibody [Unconjugated]
Immunocytochemistry EGFR Antibody [Unconjugated]
Western Blot EGFR Antibody [Unconjugated]
(30 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
RNF111 Antibody
(1C4)
H00054778-M05
ELISA RNF111 Antibody (1C4)
Western Blot RNF111 Antibody (1C4)
Immunocytochemistry/ Immunofluorescence RNF111 Antibody (1C4)
(13 Publications)
Species: Hu
Applications: ELISA, ICC/IF, KD, WB
Fibronectin
Antibody - BSA Free
NBP1-91258
Western Blot Fibronectin Antibody - BSA Free
Western Blot Fibronectin Antibody - BSA Free
Western Blot Fibronectin Antibody - BSA Free
(11 Reviews)
(56 Publications)
Species: Bv, Ca, Eq, Fe, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(818 Publications)
Species: Hu
Applications: BA
HGFR/c-MET
Antibody [Unconjugate
AF276
Immunocytochemistry HGFR/c-MET Antibody [Unconjugated]
Immunohistochemistry HGFR/c-MET Antibody [Unconjugated]
Knockout Validated HGFR/c-MET Antibody [Unconjugated]
(1 Review)
(54 Publications)
Species: Hu
Applications: Block, CyTOF-ready, Flow, ICC, IHC, KO, Simple Western, WB
RNMT Antibody
(3H3-1D12)
H00008731-M01
Immunocytochemistry/ Immunofluorescence RNMT Antibody (3H3-1D12)
Immunohistochemistry-Paraffin RNMT Antibody (3H3-1D12)
Western Blot RNMT Antibody (3H3-1D12)
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
PIK3CA Antibody
NBP2-19804
Western Blot PIK3CA Antibody
Immunohistochemistry-Paraffin PIK3CA Antibody
Western Blot PIK3CA Antibody
(4 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
SLTM Antibody
NBP2-38464
Immunohistochemistry-Paraffin SLTM Antibody
Western Blot SLTM Antibody
Immunocytochemistry/ Immunofluorescence SLTM Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
ErbB2/Her2
[Unconjugated]
1129-ER
SDS-PAGE ErbB2/Her2 [Unconjugated]
Bioactivity ErbB2/Her2 [Unconjugated]
(58 Publications)
Species: Hu
Applications: BA
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(667 Publications)
Species: Mu
Applications: ELISA
c-jun [p Ser63]
Antibody (1018Y) [Unc ...
MAB8930
Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu
Applications: ICC, Simple Western, WB
PI 3-Kinase p110
beta/PIK3CB Antibody ...
MAB2686
Western Blot PI 3-Kinase p110 beta/PIK3CB Antibody (269020)
(5 Publications)
Species: Hu
Applications: WB
PI 3-Kinase p110
gamma Antibody
NBP2-15071
Western Blot PI 3-Kinase p110 gamma Antibody
Western Blot PI 3-Kinase p110 gamma Antibody
Immunohistochemistry-Paraffin PI 3-Kinase p110 gamma Antibody
(3 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB

Publications for Axl (DYC2228-2)(3)

We have publications tested in 1 confirmed species: Human.


Filter By Application
All Applications
Filter By Species
Human
(3)
All Species

Reviews for Axl (DYC2228-2) (0)

There are no reviews for Axl (DYC2228-2). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Axl (DYC2228-2) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Axl Products

Blogs on Axl.

Check out the latest blog posts on Axl.
Microglia: pruning shears for homeostatic maintenance in the brain
By Jennifer Sokolowski, MD, PhD.Microglia play a critical role in pruning neurons and synapses during homeostatic maintenance in the adult brain.1 A recent study by Ayata et al. (2018) identified regional differe...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005
Axl was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF854) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Axl Antibody [Unconjugated]
AF854

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Review this Product

Be the first to review our Human Phospho-Axl DuoSet IC ELISA, 2 Plate and receive a gift card or discount.


Bioinformatics

Gene Symbol AXL