Lysates prepared from A431 human epithelial carcinoma cells were incubated in wells coated with Total Bcl-xL Capture Antibody. Unbound material was removed by washing and bound material was solubilized in SDS gel sample ...read more
A431 human epithelial carcinoma cells and NIH-3T3 mouse embryonic fibroblast cells were lysed as described in the Preparation of Samples section in the product insert (Catalog # DYC894). Human and mouse Bcl-xL were ...read more
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Human/Mouse Total Bcl-xL DuoSet IC ELISA, 2 Plate
Apoptosis regulator Bcl-X
bcl2-L-1
BCL2-like 1
bcl-2-like protein 1
BCLXBCL2LBcl-X
BclxL
Bcl-xL
BCL-XL/S
BCLXS
bcl-xS
DKFZp781P2092
Background
Bcl-x is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. Alternative splicing results in two distinct Bcl-x isoforms. Bcl-xL (long) is an anti-apoptotic protein that prevents release of Cytochromec from the mitochondrial intermembrane space into the cytosol. Bcl-xs (short) is a pro-apoptotic member that can initiate programmed cell death.
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