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Human Lipocalin-2/NGAL Quantikine QuicKit ELISA

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Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Human Lipocalin-2/NGAL Quantikine QuicKit ELISA Summary

Background
The Quantikine™ QuicKit™ Human Lipocalin-2/NGAL Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human Lipocalin-2 levels in cell culture supernates, serum, heparin plasma, saliva, and urine. It contains NS0-expressed recombinant human Lipocalin-2 and antibodies raised against the recombinant protein. Results obtained using natural human... Lipocalin-2 showed linear curves that were parallel to the standard curves obtained using the QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human Lipocalin-2.
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Specificity
Natural and recombinant human Lipocalin-2.
Source
N/A
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Lipocalin-2/NGAL Quantikine QuicKit ELISA

  • 24p3
  • 25 kDa alpha-2-microglobulin-related subunit of MMP-9
  • HNL
  • LCN2
  • lipocalin 2 (oncogene 24p3)
  • lipocalin 2
  • Lipocalin2
  • Lipocalin-2
  • migration-stimulating factor inhibitor
  • MSFI
  • neutrophil gelatinase-associated lipocalin
  • NGAL
  • NGALlipocalin-2
  • Oncogene 24p3
  • p25
  • Siderocalin
  • Uterocalin

Background

The Lipocalin family comprises a diverse group of mostly secreted soluble proteins that bind hydrophobic ligands and act as transporters, carrying small molecules to specific cells. Lipocalins are related by possessing an 8-stranded beta-barrel structure. Lipocalin-1, also named tear lipocalin (TL), von Ebners gland protein (VEG) and tear pre-albumin, binds a large number of hydrophobic molecules and exhibits cysteine proteinase inhibitor and endonuclear activities. Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a component of granules in neutrophils from tissues that are normally exposed to microorganisms and is upregulated during inflammation. Lipocalin-2 can form homodimers and can heterodimerize with the neutrophil gelatinase MMP-9.

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