![Western Blot: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - A. 30 ug 293T whole cell lysate/extractB. 30 ug A431whole cell lysate/extractC. 30 ug HeLa whole cell lysate/extractD. 30 ug HepG2 whole cell lysate/extract7.5 % SDS-PAGE HSC70 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Western-Blot-NBP2-16882-img0013.jpg "Western Blot: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - A. 30 ug 293T whole cell lysate/extractB. 30 ug A431whole cell lysate/extractC. 30 ug HeLa whole cell lysate/extractD. 30 ug HepG2 whole cell lysate/extract7.5 % SDS-PAGE HSC70 antibody dilution: 1:1000")
| Reactivity | Hu, Mu, Pm, RM, XpSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HSC70. The exact sequence is proprietary. |
| Localization | Cytoplasm; Melanosome |
| Predicted Species | Rhesus Macaque (100%), Primate (100%), Xenopus (97%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HSPA8 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 71 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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Enlarge |
reviewed by:
Kevin Schauer |
WB | Opsanus beta | 02/15/2017 |
Summary
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: HSC70 protein stained by HSC70 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16882-img0012.jpg "Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: HSC70 protein stained by HSC70 antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - HSC70 antibody detects HSC70 protein at cytosol on mouse prostate by immunohistochemical analysis.Sample: Paraffin-embedded mouse prostate. HSC70 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Immunohistochemistry-Paraffin-NBP2-16882-img0010.jpg "Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - HSC70 antibody detects HSC70 protein at cytosol on mouse prostate by immunohistochemical analysis.Sample: Paraffin-embedded mouse prostate. HSC70 antibody dilution: 1:500.")
![Western Blot: Hsc70 Antibody [NBP2-16882] - Sample (30 ug of whole cell lysate) A: Neuro2A B: GL261 C: C8D30 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Hsc70-Antibody-Western-Blot-NBP2-16882-img0001.jpg "Western Blot: Hsc70 Antibody [NBP2-16882] - Sample (30 ug of whole cell lysate) A: Neuro2A B: GL261 C: C8D30 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: Hsc70 Antibody [NBP2-16882] - Sample (30 ug of whole cell lysate) A: A431 B: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Hsc70-Antibody-Western-Blot-NBP2-16882-img0002.jpg "Western Blot: Hsc70 Antibody [NBP2-16882] - Sample (30 ug of whole cell lysate) A: A431 B: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Hsc70 Antibody [NBP2-16882] - Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Hsc70-Antibody-Immunohistochemistry-Paraffin-NBP2-16882-img0003.jpg "Immunohistochemistry-Paraffin: Hsc70 Antibody [NBP2-16882] - Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using antibody at 1:100 dilution.")
![Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - HSC70 antibody detects HSC70 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. HSC70 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Immunohistochemistry-Paraffin-NBP2-16882-img0005.jpg "Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - HSC70 antibody detects HSC70 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. HSC70 antibody dilution: 1:500.")
![ELISA HSP70/HSPA1A [Biotin]](https://images.novusbio.com/images2/dyc1663-2_human-mouse-rat-total-hsp70-hspa1a-duoset-ic-elisa-5-plate-elisa-109202094930.jpg)
![ELISA HSP70/HSPA1A [Biotin]](https://images.novusbio.com/images2/dyc1663-2_human-mouse-rat-total-hsp70-hspa1a-duoset-ic-elisa-5-plate-elisa-512021144631.png)
![ELISA HSP70/HSPA1A [Biotin]](https://images.novusbio.com/images2/dyc1663-2_human-mouse-rat-total-hsp70-hspa1a-duoset-ic-elisa-5-plate-elisa-512021144743.png)
![Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_6973.jpg)
![Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_9671.jpg)
![Immunoprecipitation CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412513815.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
