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DNAJC6 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: DNAJC6 Antibody [NBP1-81507] - Analysis in human cerebral cortex and pancreas tissues using Anti-DNAJC6 antibody. Corresponding DNAJC6 RNA-seq data are ...read more
Western Blot: DNAJC6 Antibody [NBP1-81507] - Analysis in human cell line HEL.
Immunocytochemistry/ Immunofluorescence: DNAJC6 Antibody [NBP1-81507] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DNAJC6 Antibody [NBP1-81507] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: DNAJC6 Antibody [NBP1-81507] - Staining of human pancreas shows low expression as expected.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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DNAJC6 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NGDKPHGVKKPSKKQQEPAAPPPPEDVDLLGLEGSAMSNSFSPPAAPPTNSELLSDLFGGGGAAGPTQAGQSGVEDVFHPSGPASTQSTP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNAJC6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
DNAJC6 Protein (NBP1-81507PEP)
Publications
Read Publication using
NBP1-81507 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (Roosen et al). Rat (84%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DNAJC6 Antibody

  • DJC6
  • DnaJ (Hsp40) homolog, subfamily B, member 6
  • DnaJ (Hsp40) homolog, subfamily C, member 6
  • DnaJ homolog subfamily C member 6
  • EC 3.1.3.48
  • KIAA0473auxilin
  • MGC129914
  • MGC129915
  • MGC48436
  • putative tyrosine-protein phosphatase auxilin

Background

DNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNAJC6 Antibody (NBP1-81507)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(1)
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Showing Publication 1 - 1 of 1.
Publication using NBP1-81507 Applications Species
Roosen DA, Landeck N, Conti M, Smith N Mutations in Auxilin cause parkinsonism via impaired clathrin-mediated trafficking at the Golgi apparatus and synapse BioRxiv (WB, Mouse) WB Mouse

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol DNAJC6