Orthogonal Strategies: Immunohistochemistry-Paraffin: DNAJC6 Antibody [NBP1-81507] - Analysis in human cerebral cortex and pancreas tissues using Anti-DNAJC6 antibody. Corresponding DNAJC6 RNA-seq data are ...read more
Western Blot: DNAJC6 Antibody [NBP1-81507] - Analysis in human cell line HEL.
Immunocytochemistry/ Immunofluorescence: DNAJC6 Antibody [NBP1-81507] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DNAJC6 Antibody [NBP1-81507] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: DNAJC6 Antibody [NBP1-81507] - Staining of human pancreas shows low expression as expected.
This antibody was developed against Recombinant Protein corresponding to amino acids: NGDKPHGVKKPSKKQQEPAAPPPPEDVDLLGLEGSAMSNSFSPPAAPPTNSELLSDLFGGGGAAGPTQAGQSGVEDVFHPSGPASTQSTP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNAJC6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Mouse reactivity reported in scientific literature (Roosen et al). Rat (84%).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for DNAJC6 Antibody
DJC6
DnaJ (Hsp40) homolog, subfamily B, member 6
DnaJ (Hsp40) homolog, subfamily C, member 6
DnaJ homolog subfamily C member 6
EC 3.1.3.48
KIAA0473auxilin
MGC129914
MGC129915
MGC48436
putative tyrosine-protein phosphatase auxilin
Background
DNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
We have publications tested in 1 confirmed species: Mouse.
We have publications tested in 1 application: WB.
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Publication using NBP1-81507
Applications
Species
Roosen DA, Landeck N, Conti M, Smith N Mutations in Auxilin cause parkinsonism via impaired clathrin-mediated trafficking at the Golgi apparatus and synapse BioRxiv (WB, Mouse)
WB
Mouse
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